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1.
Roger Cardinal 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2009,364(1522):1459-1466
Outsider Art (art brut) is defined as a mode of original artistic expression which thrives on its independence, shunning the public sphere and the art market. Such art can be highly idiosyncratic and secretive, and reflects the individual creator''s attempt to construct a coherent, albeit strange, private world. Certain practitioners of what may be termed autistic art are examined in the light of this definition; their work is considered as evidence not of a medical condition but of an expressive intentionality entirely worthy of the interest of those drawn to the aesthetic experience. 相似文献
2.
It has been suggested that Locusta migratoria amplifies its ribosomal RNA genes in the growing oocytes (Kunz (1967) Chromosoma20, 332–370). Cloned ribosomal DNA of L. migratoria was used to analyze rDNA structure and number. The rDNA is localized on three chromosome pairs in six nucleolus organizers. It was found that all structural variants of the rRNA genes which have been described previously are represented in the same relative amounts in DNA from isolated oocytes as in somatic cells. Furthermore, the rRNA gene number is not increased in oocyte DNA, i.e., amplification does not occur. Therefore, the large number of multiple nucleoli seen in the growing oocytes has to be interpreted as the fully extended and fully active set of chromosomal rRNA genes. The total rRNA gene number was determined by dot blot hybridization to be about 3300 genes/haploid genome. 相似文献
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Summary Immunohistochemical properties of the terminal nerve network in the rat heart were assessed by use of the elution-restaining method. The colocalization of the enzymes involved in catecholamine synthesis (tyrosine hydroxylase — TH, dopamine--hydroxylase — DBH) as well as the respective distributions of the neuropeptides associated with the adrenergic nervous system (neuropeptide tyrosine — NPY, C-terminal flanking peptide of neuropeptide Y — C-PON) were studied in series of serial sections throughout the interatrial septum and the atrioventricular junction. Our data suggest that ganglion cells of sulcus terminalis as well as the epicardial ganglia enclosed between the superior vena cava and ascending aorta are VIP- and TH-negative, but neuropeptide Y- and DBH-immunoreactive. They give rise to three intraseptal nerves directed towards the specialised structures of the atrioventricular junction. These nerve fascicles contain abundant, thick TH-immunoreactive nerve fibres and scarce, thin NPY- and DBH-immunoreactive fibres. The cell bodies of the intramural ganglion cells localized between the right and left branches of the bundle of His (Moravec and Moravec 1984) are strongly TH- and DBH-immunoreactive. They are innervated by thick nerve fibres having the same immunohistochemical properties (NPY- and DBH-immunoreactivities) as those of a subpopulation of the epicardial ganglion cells and seem to supply some of the TH-immunoreactive nerve fibres directed via the intraseptal nerves to the epicardial ganglia. The existence of a multicomponent nerve network, characterized by a reciprocal innervation of the sinus node and atrioventricular node areas, is suggested by our immunohistochemical data. 相似文献
5.
Cystic fibrosis typing with DNA probes and screening for ΔF508 deletion in families from Southern France 总被引:1,自引:1,他引:0
Mireille Claustres Marie Desgeorges Paule Kjellherg Hélène Bellet Jacques Demaille Michelle Ramsay 《Human genetics》1990,85(4):398-399
Summary A sample of 235 individuals from 49 French cystic fibrosis (CF) families with at least one living affected child was typed
with probes for restriction fragment length polymorphisms (RFLPs) known to be linked to the CF gene, and was screened for
the ΔF508 mutation. Using a combination of six probes, 44 out of the 49 families were sufficiently informative to enable prenatal
diagnosis or carrier determination. As in many other populations, linkage disequilibrium was found between the CF locus and
the haplotype B (XV2c: allele 1; KM19: allele 2), which accounts for about 78% of CF chromosomes in our families. The ΔF508
deletion was present in 64.3% of CF chromosomes. 相似文献
6.
The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function 总被引:6,自引:0,他引:6
Dr. Nancy L. Parenteau Patrick Bilbo Cynthia J. M. Nolte Valerie S. Mason Mireille Rosenberg 《Cytotechnology》1992,9(1-3):163-171
We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal
fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required
the use of traditional as well as specialized culture techniques. The configuration of the construct has been engineered to
maintain polarity and permit extended culture at the air-liquid interface. Morphological, biochemical and kinetic parameters
were assessed and functional assays were performed to determine the degree of similarity to human skin. Light and ultrastructural
morphology of the epidermis closely resembled human skin. The immunocytochemical localization of a number of differentiation
markers and extracellular matrix proteins was also similar to human skin. Kinetic data showed a transition of the epidermal
layer to a morein vivo-like growth rate during the development of the construct at the air-liquid interface. The barrier properties of the construct
also increased with time reaching a permeability to water of less than 2%·h after approximately 2 weeks at the air-liquid
interface which is still on average 30-fold more water-permeable than normal human skin. The construct is currently used forin vitro research and testing and is also being tested in clinical applications. 相似文献
7.
Underwater effects on coral reefs of the six hurricanes which ravaged French Polynesia between December 82 and April 83 were observed by SCUBA diving around high islands and atolls during September and October 1983. Special attention was paid to Tikehau atoll reef formations (Tuamotu archipelago) where quantitative studies on scleractinians, cryptofauna and fishes were conducted in 1982 immediatly prior to the hurricanes. On outer reef slopes coral destruction, varying from 50 to 100%, was a function of depth. Upper slope coral communities composed of small colonies well adapted to high energy level environments, suffered less than deeper formations. However, there is a narrow erosional trough in this zone at a depth of 6 m that was probably the result of storm-wave action (plunge point). Coral destruction was spectacular at depths greater than 12 m: 60 to 80% between 12 m and 30 m and 100% beyond 35 m, whereas earlier living coral coverage ranged from 60 to 75% in these zones. The outer slope was transformed into a scree zone covered with coarse sand and dead coral rubble. Dives on different sites around steep outer slopes (>45°) of the atolls and more gentle slopes (<25°) of some parts of the high islands permitted the formulation of an explanatory hypothesis: direct coral destruction by hurricane-induced waves occurred between the surface and 18–20 m; on low-angle slopes broken colonies were thrown up on reef flats and beaches; on steep slopes avalanches destroyed much of the living corals and left scree slopes of rubble and sand. 相似文献
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10.
Michal Jarnik Jiang-Qing Tang Maria Korab-Laskowska Ewa Zie
tkiewicz Guy Cardinal Izabella Gorska-Flipot Daniel Sinnett Damian Labuda 《Genomics》1996,36(3):388
We studied two systems of multilocus markers revealed by PCR using primers directing amplification betweenAlurepeats in a tail-to-tail orientation. Genomic polymorphisms were detected as the presence or absence of the electrophoretic bands representing DNA fragments of a given length. A total of 104 such fragments segregating as Mendelian markers in a panel of eight CEPH families were analyzed by two-point linkage analysis. Fifty-one of these fragments were localized with respect to CEPH markers; they represented 33 loci, 7 of which were multiallelic. Locus-specific oligonucleotides were developed and used as hybridization probes to identify the mapped loci within a complex pattern of inter-AluPCR products. A great proportion of inter-AluPCR polymorphisms represented length variants within amplified DNA segments, while others were presumably due to mutations within the priming sites. To describe the expected number of informative loci per typing experiment we introduced a parameter called overall informativity (OI), which provides a single measure of the multiplex ratio and the informativity of markers contributing to a multilocus system (OIof a single locus is equivalent to its heterozygosity and cannot exceed 0.5 for a biallelic codominant marker). HighOIvalues (5.8 and 11.5) of the two presented systems of inter-AluPCR markers of random chromosomal distribution render them suitable for mapping genomic rearrangements such as genomic deletions in tumoral tissues. This was illustrated by the detection of loss of heterozygosity in the 9q22–qter region in sporadic colon cancer. 相似文献