全文获取类型
收费全文 | 991篇 |
免费 | 89篇 |
出版年
2023年 | 5篇 |
2022年 | 9篇 |
2021年 | 7篇 |
2020年 | 18篇 |
2019年 | 21篇 |
2018年 | 32篇 |
2017年 | 27篇 |
2016年 | 29篇 |
2015年 | 54篇 |
2014年 | 52篇 |
2013年 | 75篇 |
2012年 | 102篇 |
2011年 | 76篇 |
2010年 | 41篇 |
2009年 | 45篇 |
2008年 | 60篇 |
2007年 | 52篇 |
2006年 | 42篇 |
2005年 | 39篇 |
2004年 | 42篇 |
2003年 | 36篇 |
2002年 | 47篇 |
2001年 | 16篇 |
2000年 | 18篇 |
1999年 | 15篇 |
1998年 | 13篇 |
1997年 | 13篇 |
1996年 | 9篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 9篇 |
1990年 | 7篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1974年 | 2篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 4篇 |
1968年 | 2篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1965年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有1080条查询结果,搜索用时 31 毫秒
1.
Legionella pneumophila in Cooling Towers: Fluctuations in Counts, Determination of Genetic Variability by Pulsed-Field Gel Electrophoresis (PFGE), and Persistence of PFGE Patterns
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sonia Ragull Marian Garcia-Nuez Maria Luisa Pedro-Botet Nieves Sopena Maria Esteve Rafael Montenegro Miquel Sabri 《Applied microbiology》2007,73(16):5382-5384
The concentrations of Legionella pneumophila in cooling towers may vary considerably over short periods of time, producing significant fluctuations throughout the year. Despite genetic variability, in small geographical areas the same indistinguishable pulsed-field gel electrophoresis patterns may be shared among different cooling towers and persist over time. 相似文献
2.
3.
4.
The isolated molecular forms of guinea-pig skeletal muscle cytoplasmic malate dehydrogenase have a different chromatographic behaviour through affinity or hydrophobic interaction gels; in all cases the retention of the B form is more noticeable. Chromatography of a partly purified preparation through 5' AMP-Sepharose allows both molecular forms of malate dehydrogenase to be separated and obtained free from lactate dehydrogenase. 相似文献
5.
6.
7.
8.
Summary Genes as POT1, HSP104 and SSA3, which are late expressed in laboratory culture conditions are expressed only during the first few days in microvinifications in wine yeast cells. This effect is probably due to the different growth conditions and leads to useless levels of enzyme activity for a reporter gene. However the ACT1 promoter, which is constitutively expressed in laboratory conditions, produces sufficient amounts of enzyme activity in late fermentation phases. 相似文献
9.
10.
It is usually thought that unlike terrestrial plants, phytoplankton will not show a significant response to an increase of atmospheric CO2. Here we suggest that this view may be biased by a neglect of the effects of carbon (C) assimilation on the pH and the dissociation of the C species. We show that under eutrophic conditions, productivity may double as a result of doubling of the atmospheric CO2 concentration. Although in practice productivity increase will usually be less, we still predict a productivity increase of up to 40% in marine species with a low affinity for bicarbonate. In eutrophic freshwater systems doubling of atmospheric CO2 may result in an increase of the productivity of more than 50%. Freshwaters with low alkalinity appeared to be very sensitive to atmospheric CO2 elevation. Our results suggest that the aquatic C sink may increase more than expected, and that nuisance phytoplankton blooms may be aggravated at elevated atmospheric CO2 concentrations. 相似文献