全文获取类型
收费全文 | 26849篇 |
免费 | 2472篇 |
国内免费 | 2826篇 |
出版年
2024年 | 92篇 |
2023年 | 395篇 |
2022年 | 859篇 |
2021年 | 1325篇 |
2020年 | 975篇 |
2019年 | 1228篇 |
2018年 | 1178篇 |
2017年 | 881篇 |
2016年 | 1197篇 |
2015年 | 1761篇 |
2014年 | 2125篇 |
2013年 | 2104篇 |
2012年 | 2580篇 |
2011年 | 2384篇 |
2010年 | 1431篇 |
2009年 | 1307篇 |
2008年 | 1594篇 |
2007年 | 1426篇 |
2006年 | 1147篇 |
2005年 | 983篇 |
2004年 | 858篇 |
2003年 | 757篇 |
2002年 | 726篇 |
2001年 | 405篇 |
2000年 | 356篇 |
1999年 | 337篇 |
1998年 | 238篇 |
1997年 | 192篇 |
1996年 | 160篇 |
1995年 | 124篇 |
1994年 | 136篇 |
1993年 | 91篇 |
1992年 | 112篇 |
1991年 | 102篇 |
1990年 | 83篇 |
1989年 | 55篇 |
1988年 | 56篇 |
1987年 | 34篇 |
1986年 | 45篇 |
1985年 | 42篇 |
1984年 | 28篇 |
1983年 | 33篇 |
1982年 | 21篇 |
1980年 | 16篇 |
1979年 | 18篇 |
1978年 | 12篇 |
1977年 | 13篇 |
1975年 | 18篇 |
1974年 | 15篇 |
1970年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Yunfan Sun Liang Wu Yu Zhong Kaiqian Zhou Yong Hou Zifei Wang Zefan Zhang Jiarui Xie Chunqing Wang Dandan Chen Yaling Huang Xiaochan Wei Yinghong Shi Zhikun Zhao Yuehua Li Ziwei Guo Qichao Yu Liqin Xu Jia Fan 《Cell》2021,184(2):404-421.e16
- Download : Download high-res image (233KB)
- Download : Download full-size image
2.
3.
4.
Peng Wang Ronghua Luo Min Zhang Yaqing Wang Tianzhang Song Tingting Tao Zhongyu Li Lin Jin Hongyi Zheng Wenwen Chen Mengqian Zhao Yongtang Zheng Jianhua Qin 《Cell death & disease》2020,11(12)
COVID-19, caused by SARS-CoV-2, is an acute and rapidly developing pandemic, which leads to a global health crisis. SARS-CoV-2 primarily attacks human alveoli and causes severe lung infection and damage. To better understand the molecular basis of this disease, we sought to characterize the responses of alveolar epithelium and its adjacent microvascular endothelium to viral infection under a co-culture system. SARS-CoV-2 infection caused massive virus replication and dramatic organelles remodeling in alveolar epithelial cells, alone. While, viral infection affected endothelial cells in an indirect manner, which was mediated by infected alveolar epithelium. Proteomics analysis and TEM examinations showed viral infection caused global proteomic modulations and marked ultrastructural changes in both epithelial cells and endothelial cells under the co-culture system. In particular, viral infection elicited global protein changes and structural reorganizations across many sub-cellular compartments in epithelial cells. Among the affected organelles, mitochondrion seems to be a primary target organelle. Besides, according to EM and proteomic results, we identified Daurisoline, a potent autophagy inhibitor, could inhibit virus replication effectively in host cells. Collectively, our study revealed an unrecognized cross-talk between epithelium and endothelium, which contributed to alveolar–capillary injury during SARS-CoV-2 infection. These new findings will expand our understanding of COVID-19 and may also be helpful for targeted drug development.Subject terms: Mechanisms of disease, Viral infection 相似文献
5.
6.
Jing Hu Wail M. Hassan Guorong Chen Yanlin Li Wenqiao Fan Wei Wang 《Ecological Entomology》2019,44(6):800-809
1. The Qilian Mountains represent one of the key livestock‐raising grasslands in China. The two main herbivore species raised in this area – yaks and sheep – are of critical economical value. Grasshoppers compete with these animals for available nutrients, creating multifaceted relationships between livestock, grasshoppers and plants. A clear understanding of such relationships is lacking and is urgently needed to guide conservation efforts. 2. This study aims to document the effects of yak and sheep grazing on grasshopper assemblages and to elucidate the underlying mechanisms of such effects. 3. It is shown here that yaks and sheep impact grasshopper assemblages differently. Grasshopper assemblages exhibited lower density, biodiversity, richness, and evenness of distribution in yak‐grazed pastures than in grazing‐free grasslands. Sheep‐grazed pastures exhibited a dramatically divergent picture, with elevated density, biodiversity and richness, and a slightly decreased evenness of distribution. Grasshoppers were generally larger in grazed pastures than in grazing‐free grasslands, especially in yak‐grazed plots. 4. The present study suggests that differences between yak and sheep pastures in plant assemblage structure and plant traits are probably the underlying forces driving the differences in grasshopper assemblage structure and grasshopper traits, respectively. 5. The study shows that the grasshopper habitat indicator species differ between yak and sheep pastures, raising the possibility that such indicators can be used to monitor grassland usage and degradation in the Qilian Mountains. 6. These results provide novel insights into the dynamic interactions of common domesticated herbivore species, grasshoppers and plants in Qilian Mountains, which augment current knowledge and may ultimately lead to better conservation practices. 相似文献
7.
Xu Liu Ben Chen Lulan Chen Wan-Ting Ren Juan Liu Guoxiang Wang Wei Fan Xin Wang Yun Wang 《PloS one》2013,8(4)
Phenol red is widely used in cell culture as a pH indicator. Recently, it also has been reported to have estrogen-like bioactivity and be capable of promoting cell proliferation in different cell lines. However, the effect of phenol red on primary neuronal culture has never been investigated. By using patch clamp technique, we demonstrated that hippocampal pyramidal neurons cultured in neurobasal medium containing no phenol red had large depolarization-associated epileptiform bursting activities, which were rarely seen in neurons cultured in phenol red-containing medium. Further experiment data indicate that the suppressive effect of the phenol red on the abnormal epileptiform burst neuronal activities was U-shape dose related, with the most effective concentration at 28 µM. In addition, this concentration related inhibitory effect of phenol red on the epileptiform neuronal discharges was mimicked by 17-β-estradiol, an estrogen receptor agonist, and inhibited by ICI-182,780, an estrogen receptor antagonist. Our results suggest that estrogen receptor activation by phenol red in the culture medium prevents formation of abnormal, epileptiform burst activity. These studies highlight the importance of phenol red as estrogen receptor stimulator and cautions of careful use of phenol red in cell culture media. 相似文献
8.
zge Karayel Francesca Tonelli Sebastian Virreira Winter Phillip E. Geyer Ying Fan Esther M. Sammler Dario R. Alessi Martin Steger Matthias Mann 《Molecular & cellular proteomics : MCP》2020,19(9):1546-1560
- Download : Download high-res image (63KB)
- Download : Download full-size image
- •MS-based clinical assay that accurately determines phospho Rab10 occupancy.
- •Stable isotope labeled phosphopeptide injected as a standard with endogenous tryptic phospho Rab peptide for accurate ratio determination.
- •Determination of pRab levels in neutrophils of Parkinson disease patients.
- •Relevance of pRab levels as marker of PD.
9.
10.