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排序方式: 共有377条查询结果,搜索用时 15 毫秒
1.
L. A. Mikhailova 《Human physiology》2001,27(6):680-685
We studied 654 healthy ten- to twelve-year-old children living in the Russian Far North (Taimyr peninsula) and in Siberia (town of Krasnoyarsk). The state of the body oxygenation and the systems responsible for oxygen delivery were studied according to the length of time living in the Russian Far North and season of the year. An examination of newcomer children revealed the following changes during the first year of living in the Russian Far North: an increased respiration rate and changes in its circadian rhythm; an increase in the heart rate, cardiac output, and cardiac index; an increase in the mean corpuscular volume in the red blood system; the activation of lipid peroxidation, and the changes in electrolyte balance of cells. The control mechanisms of oxygen transport system gradually stabilized during the ages of two to five years. In children living more than five years in the Russian Far North, the increased oxygen demand was met by the activation of cardiovascular and red blood systems aimed at more effective and adequate oxygen delivery to tissues. 相似文献
2.
The leucocyte counts in the peripheral blood and titers of the serum lysozyme under conditions of various experimental models were compared. Albino rats, mice, guinea pigs and rabbits exposed to the effect of various factors of a biological and chemical nature were used in the experiments. In addition, observations on humans immunized with bacterial and viral vaccines were analyzed. Development of 3 reaction types was shown to be possible. In the 1st type the dynamics of the lysosome activity and blood leucocyte counts changed in the same way (immunization with smallpox vaccine, listeria infection). Decreased counts of the leucocytes due to the use of cytostatic drugs were accompanied by a parallel decrease in the lysozyme activity. Such variant of the reaction was predominating. The 2nd type of the shifts characterized by an impaired synchronous pattern of the dynamics of the indices was not so frequent (the use of high doses of cytostatics). The 3rd type of the reactions with an exactly opposite character of the shifts in the leucocyte counts and lysozyme activity was observed only as an exclusion. It is concluded that the quantitative dynamics of the leucocytes is not enough by itself for creating an opinion on the nature of the changes in the serum lysozyme. The possible role of macrophages in the above processes is discussed. 相似文献
3.
4.
Mateikovich A. P. Punina E. O. Kopylov-Guskov Yu. O. Nosov N. N. Gudkova P. D. Gnutikov A. A. Machs E. M. Mikhailova Yu. V. Krapivskaya E. E. Rodionov A. V. 《Russian Journal of Genetics》2020,56(4):417-428
Russian Journal of Genetics - The ITS1–5.8S rDNA–ITS2 sequence of the 35S rRNA genes of 16 species of feather grasses and 2 species of false needlegrasses of the flora of the Altai... 相似文献
5.
Nina Mironenko Elena Timopheeva Ludmila Mikhailova Doris Kopahnke Ilona Krämer Frank Ordon 《Archives Of Phytopathology And Plant Protection》2013,46(6):431-440
Abstract Random amplified polymorphic DNAs (RAPDs) were used to study the genetic variation of Pyrenophora tritici-repentis isolates causing wheat tan spot. Two independent experiments were conducted in 2002 – 2003. In 2002, 40 isolates collected in Russia (Krasnodar region, Bashkiria), Germany, and the Czech Republic were studied and 35 unique RAPD genotypes were identified. Most of the genetic variation (72%) was observed within populations and 28% between them. In 2003, 69 new isolates from Russia (Dagestan, North Osetia, Bashkiria), Germany, and the Czech Republic were studied and 47 unique RAPD genotypes were identified. As in 2002, most of the genetic variation (75%) was observed within populations and 25% between them. Total gene diversity in each group ranged from 0.67 – 1.00 for 2002 and was 1.00 for 2003. The average gene diversity was estimated between 0.13 and 0.20 in 2002 and between 0.07 and 0.18 in 2003. A dendrogramme based on genetic distances between isolates illustrates that the variation is distributed on a small scale (0.3 – 4.0%). Estimated FST values and clustering of isolates on dendrogrammes suggest that groups of isolates from Bashkiria and groups of isolates from Dagestan and North Osetia are separated from others and may be considered as different geographical populations. No clear differentiation between isolates from other sites was revealed. 相似文献
6.
N. Yu. Gerasimenko A. V. Slavutskaya S. A. Kalinin E. S. Mikhailova 《Human physiology》2013,39(3):248-255
Psychophysiological experiments were performed on 34 healthy subjects. We analyzed the accuracy and latency of motor response in recognizing two types of complex visual stimuli, animals and objects, which were presented immediately after a brief presentation of face images with different emotional expressions: anger, fear, happiness, and a neutral expression. We revealed the dependence of response latency on emotional expression of the masked face. The response latency was lower when the test stimuli were preceded by angry or fearful faces compared to happy or neutral faces. These effects depended on the type of stimulus and were more expressive when recognizing objects compared to animals. We found that the effects of emotional faces were related to personal features of the subjects that they exhibited in the emotional and communicative blocks of Cattell’s test and were more expressive in more sensitive, anxious, and pessimistic introverts. The mechanisms of the effects of unconsciously perceived emotional information on human visual behavior are discussed. 相似文献
7.
Lovisa Wennerström Linda Laikre Nils Ryman Fred M. Utter Nurul Izza Ab Ghani Carl André Jacquelin DeFaveri Daniel Johansson Lena Kautsky Juha Merilä Natalia Mikhailova Ricardo Pereyra Annica Sandström Amber G. F. Teacher Roman Wenne Anti Vasemägi Małgorzata Zbawicka Kerstin Johannesson Craig R. Primmer 《Biodiversity and Conservation》2013,22(13-14):3045-3065
Information on spatial and temporal patterns of genetic diversity is a prerequisite to understanding the demography of populations, and is fundamental to successful management and conservation of species. In the sea, it has been observed that oceanographic and other physical forces can constitute barriers to gene flow that may result in similar population genetic structures in different species. Such similarities among species would greatly simplify management of genetic biodiversity. Here, we tested for shared genetic patterns in a complex marine area, the Baltic Sea. We assessed spatial patterns of intraspecific genetic diversity and differentiation in seven ecologically important species of the Baltic ecosystem—Atlantic herring (Clupea harengus), northern pike (Esox lucius), European whitefish (Coregonus lavaretus), three-spined stickleback (Gasterosteus aculeatus), nine-spined stickleback (Pungitius pungitius), blue mussel (Mytilus spp.), and bladderwrack (Fucus vesiculosus). We used nuclear genetic data of putatively neutral microsatellite and SNP loci from samples collected from seven regions throughout the Baltic Sea, and reference samples from North Atlantic areas. Overall, patterns of genetic diversity and differentiation among sampling regions were unique for each species, although all six species with Atlantic samples indicated strong resistence to Atlantic-Baltic gene-flow. Major genetic barriers were not shared among species within the Baltic Sea; most species show genetic heterogeneity, but significant isolation by distance was only detected in pike and whitefish. These species-specific patterns of genetic structure preclude generalizations and emphasize the need to undertake genetic surveys for species separately, and to design management plans taking into consideration the specific structures of each species. 相似文献
8.
T. R. Amen E. V. Mikhailova V. V. Alenin A. V. Artyomov P. A. Dementyev M. A. Khodorkovskii T. O. Artamonova I. M. Kuznetsova T. R. Soidla O. V. Nevzglyadova 《Cell and Tissue Biology》2013,7(1):86-94
Structural and functional characteristics of the yeast red pigment (product of polymerization of N1-(β-D-ribofuranosyl)-5-aminoimidazole), isolated from ade1 mutant cells of Saccharomyces cerevisiae and its deribosylated derivatives (obtained by acid hydrolysis) and its synthetic pigment analogue (product of polymerization of N1-methyl-5-aminoimidazole in vitro) were obtained. Products of in vitro polymerization were identified using mass spectrometry. The ability of these pigments to inhibit amyloid formation using insulin fibrils was compared. All the studied compounds are able to interact with amyloids and inhibit their growth. Electron and atomic force microscopy revealed a common feature inherent in the insulin fibrils formed in the presence of these compounds—they are merged into conglomerates more stable and resistant to the effects of ultrasound than are insulin aggregates grown without pigments. We suggest that all these compounds can cause coalescence of fibrils partially blocking the loose ends and, thereby, inhibit attachment of monomers and formation of new fibrils. 相似文献
9.
O. V. Nevzglyadova A. V. Artemov E. V. Mikhailova O. G. Lyublinskaya Yu. E. Ozerova P. A. Ivanova E. I. Kostyleva T. R. Soidla 《Cell and Tissue Biology》2016,10(4):264-276
Two transgenic yeast strains expressing human α-synuclein were used to study the impact of yeast red pigment exhibiting antiamyloid properties. It has been demonstrated that the endogenous red pigment produced under special conditions in strains carrying an ade1 mutation inhibits the expression of the hybrid protein α-synuclein-GFP. This was evident from the reduced mean value of GFP fluorescence and diminished number of cells accumulating cytoplasmic inclusions of α-synuclein-GFP. Exogenous forms of the purified red pigment (natural, synthetic and hydrolyzed derivatives) differ from the endogenous red pigment by their effect on α-synuclein. Exogenous red pigments increased the number of both cells expressing GFP fluorescence and those containing cytoplasmic inclusions. However, both endogenous and exogenous red pigments reduced the cloned α-synuclein toxicity and resulted in redistribution of the α-synuclein in cells. α-Synuclein content decreased in cell lysate pellets and increased in supernatants. 相似文献
10.
Mariya M. Mikhailova Andrey A. Panteleyev Mikhail A. Paltsev Andrey A. Panteleyev 《Cell biology international》2019,43(10):1193-1200
It is a well‐known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g., co‐culture of neural and endothelial cells) to in vivo imaging of central neural system recovery in laboratory animals after artificially induced trauma. Nevertheless, most of these models have serious limitations. Here, we describe an ex vivo model, representing an organotypic co‐culture of aortic fragments (AF) with longitudinal slices of mouse neonatal spinal cord (SC) or dorsal root ganglia (DRG). The samples were co‐cultured in a medium adapted for SC tissue and lacking any pro‐angiogenic or neurotrophic growth factors. It was found, that cultivation of AFs in the SC injury zone (transversal dissection of a SC slice) resulted in the initiation of active aortic sprouting. Remarkably, the endothelial cells exiting the AFs never invaded the SC tissue, concentrating in a nearby area (negative taxis). In contrast, the DRGs, while also promoting the sprouting, were a target of active endothelial CD31+ cell invasion (positive taxis). Thus, the tissues of both central and peripheral nervous systems have a prominent positive effect on aortic sprouting, while the vector of endothelial cell expansion is strictly nervous‐tissue‐type dependent. The ex vivo AF co‐culture with SC or DRG appeared to be a useful and promising model for a further endeavor into the mechanisms driving the complex interactions between neural and endothelial tissues. 相似文献