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Immunohistochemical localization of gamma-aminobutyric acid (GABA) in the rat pancreas 总被引:2,自引:0,他引:2
The localization of gamma-aminobutyric acid (GABA) in rat pancreas was investigated using antiserum raised against GABA conjugated to bovine serum albumin with glutaraldehyde. Immunoreactive cells were only found in the center of the pancreatic islets, and these cells were surrounded by nonimmunoreactive cells. When two serial sections of rat pancreas were consecutively stained with GABA antiserum and with antibodies against insulin, both antisera stained the same population of endocrine cells within the islets. In rats pretreated with streptozotocin, a B-cell toxin, we observed a marked decrease in the number of cells exhibiting GABA-like immunoreactivity. These observations indicate that GABA is present in the B cells of rat pancreatic islets. 相似文献
3.
The development of mitochondrial NAD+ -malate dehydrogenase (EC 1.1.1.37) in mung bean and cucumber cotyledons was followed. using the antibody raised against it, during and following germination. The developmental patterns were quite different between the two. In cucumber, the content of mitochondrial malate dehydrogenase continued to increase through 3–4 days after the beginning of imbibition. This was, at least in part, due to active synthesis of the enzyme protein, and the synthesis seemed to be regulated by the availability of the translatable mRNA for the enzyme. In mung bean, on the other hand, the enzyme was present in dry cotyledons at a rather high concentration, and remained at a constant level between day 1 and day 3 after the reduction of the content to one-half its initial level during the first day. De novo synthesis of the enzyme could not be detected in mung bean cotyledons by pulse-labeling experiment. 相似文献
4.
Studies on DNA markers (D4S10 and D4S43/S127) genetically linked to Huntington's disease in Japanese families 总被引:1,自引:0,他引:1
Ichiro Kanazawa Ikuko Kondo Joh-E Ikeda Teruaki Ikeda Yuichior Shizu Mitsuo Yoshida Hirotaro Narabayashi Shigetoshi Kuroda Hisayuki Tsunoda Eiji Mizuta Yoko Okuno Kiyotaka Sugawara Miho Murata Mafuyu Takahashi James F. Gusella 《Human genetics》1990,85(3):257-260
Summary This is the first full report on the genetic linkage between Japanese Huntington's disease and the DNA markers D4S10 and D4S43/S127. With use of the HindIII, BglI, and EcoRI polymorphisms detected at D4S10, and the combination of all these polymorphisms to give composite haplotypes, nine Japanese Huntington's disease families were found to be informative. Three recombinants for D4S10 were detected in these families, giving a maximum lod score of 1.662 at a of 0.10. Similarly, when we used the MspI and PvuII polymorphisms detected by D4S43/S127, five families gave informative results. No recombinant was detected in these families, giving a maximum lod score of 3.348 at a of 0.00. These results clearly support the view that the Japanese Huntington's disease gene may be identical with the Western gene, in spite of the lower prevalence rate in Japan. 相似文献
5.
Shoot cultures of Glehnia littoralis F. Schmidt ex Miq. (Umbelliferae) were established by placing shoot tip explants on Linsmaier and Skoog medium with 1 M NAA and 10 M BAP. Shoots were multiplied on the basal medium supplemented with 0.3 M NAA and 3 M BAP and rooted on medium containing either 1 M IBA or 3–10 M IAA. Plantlets survived in pots without any covering. This unique characteristic of the plantlets was ascribed partly to a well-developed cuticle on the surface of the leaf and the small ratio of surface area to fresh weight of a leaf blade in comparison with those of other species whose plantlets needed coverings after potting. The regenerated plantlets were finally transferred to soil.Abbreviations IAA
potassium indole-3-acetate
- IBA
indole-3-butyric acid
- IPA
indole-3-propionic acid
- NAA
potassium 1-naphthaleneacetate
- 2,4-D
sodium 2,4-dichlorophenoxyacetate
- BAP
6-benzylaminopurine
- 2-iP
N6-(2-isopentenyl)adenine 相似文献
6.
Complete nucleotide sequence of the mitochondrial DNA from a liverwort,Marchantia polymorpha 总被引:1,自引:1,他引:0
Kenji Oda Katsuyuki Yamato Eiji Ohta Yasukazu Nakamura Miho Takemura Naoko Nozato Kinya Akashi Takeshi Kanegae Yutaka Ogura Takayuki Kohchi Kanji Ohyama 《Plant Molecular Biology Reporter》1992,10(2):105-163
Libraries of cosmid and plasmid clones covering the entire region of mtDNA from the liverwortMarchantia polymorpha were constructed. These clones were used for the determination of the complete nucleotide sequence of the liverwort mtDNA
totally 186,608 bp (GenBank no. M68929) and including genes for 3 species of ribosomal RNAs, 29 genes for 27 species of transfer
RNAs, and 30 genes for functionally known proteins (16 ribosomal proteins, 3 subunits of cytochromec oxidase, apocytochromeb protein, 3 subunits of H+-ATPase, and 7 subunits of NADH ubiquinone oxidoreductase). The genome also contains 32 unidentified open reading frames.
Thus the complete nucleotide sequences from both chloroplast and mitochondrial genomes have been determined in the same organism.
Plasmid clones are available upon the request.
Gene names are represented according to Lonsdale and Leaver (1988) with modifications recommended by Lonsdale (personal communication). 相似文献
7.
Mitsuaki Kameko Miho Ichikawa Tsutomu Katsuyama Masamitsu Kanai Michimasa Kato Taiji Akamatsu 《The Histochemical journal》1986,18(4):164-168
Summary This study was undertaken, employing the immunoenzyme method, to confirm the presence of retinol-binding protein in human pancreatic islets, and to compare its distribution with that of prealbumin, insulin, glucagon, somatostatin and pancreatic polypeptide. It was found that most islet cells contained retinol-binding protein, although centrally located cells showed stronger reactivity than those in the peripheral region. The distribution of each of the five polypeptides differed from that of retinolbinding protein, indicating that these peptides did not cross-react with anti-retinol-binding protein antibody. Islet cells which contained prealbumin, on the other hand, were mostly classified as A cells. Further studies are necessary to confirm whether the islet cells produce retinol-binding protein or only store it. 相似文献
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