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排序方式: 共有370条查询结果,搜索用时 15 毫秒
1.
Richard B. Pouw Mieke C. Brouwer Judy Geissler Laurens V. van Herpen Sacha S. Zeerleder Walter A. Wuillemin Diana Wouters Taco W. Kuijpers 《PloS one》2016,11(3)
The major human complement regulator in blood, complement factor H (FH), has several closely related proteins, called FH-related (FHR) proteins. As all FHRs lack relevant complement regulatory activity, their physiological role is not well understood. FHR protein 3 (FHR-3) has been suggested to compete with FH for binding to Neisseria meningitidis, thereby affecting complement-mediated clearance. Clearly, the in vivo outcome of such competition greatly depends on the FH and FHR-3 concentrations. While FH levels have been established, accurate FHR-3 levels were never unequivocally reported to date. Moreover, CFHR3 gene copy numbers commonly vary, which may impact the FHR-3 concentration. Hence, we generated five anti-FHR-3 mAbs to specifically measure FHR-3 in human healthy donors of which we determined the gene copy number variation at the CFH/CFHR locus. Finally, we examined the acute-phase response characteristics of FHR-3 in a small sepsis cohort. We determined FHR-3 levels to have a mean of 19 nM and that under normal conditions the copy number of CFHR3 correlates to a very large extent with the FHR-3 serum levels. On average, FHR-3 was 132-fold lower compared to the FH concentration in the same serum samples and FHR-3 did not behave as a major acute phase response protein. 相似文献
2.
W. J. Middelhoven Mieke C. Hoogkamer-Te Niet W. T. A. M. De Laat C. Weijers C. J. E. A. Bulder 《Antonie van Leeuwenhoek》1986,52(6):525-535
The maximum growth rate of Trichosporon cutaneum CBS 8111 in chemostat cultures was 0.185 h-1 on ethylamine and 0.21 h-1 on butylamine, that of Candida famata CBS 8109 was 0.32 h-1 on putrescine.The amine oxidation pattern of the ascomycetous strains studied, viz. Candida famata CBS 8109, Stephanoascus ciferrii CBS 4856 and Trichosporon adeninovorans CBS 8244 was independent of the amine that had been used as the growth substrate. It resembled that of benzylamine/putrescine oxidase found in other ascomycetous yeasts. However, differences in pH optimum and substrate specificity were observed between the amine-oxidizing systems of these three species.The amine oxidation pattern of cell-free extracts of Trichosporon cutaneum CBS 8111 varied with the amine that was used as growth substrate. The enzyme system produced by Cryptococcus laurentii CBS 7140 failed to oxidize isobutylamine and benzylamine, and showed a high pH optimum.The synthesis of amine oxidase in the four yeast strains studied was not repressed by ammonium chloride and was weakly repressed by glucose but was strongly repressed if both compounds were present in the growth medium. 相似文献
3.
Molecular cloning of a gene involved in glucose sensing in the yeast Saccharomyces cerevisiae 总被引:6,自引:1,他引:5
Linda Van Aelst Stefan Hohmann Botchaka Bulaya Wim de Koning Laurens Sierkstra Maria José Neves Kattie Luyten Rafael Alijo José Ramos Paola Coccetti Enzo Martegani Neuza Maria de Magalhães-Rocha Rogelio Lopes Brandão Patrick Van Dijck Mieke Vanhalewyn Peter Durnez Arnold W. H. Jans Johan M. Thevelein 《Molecular microbiology》1993,8(5):927-943
4.
Mieke Blows 《The Australian journal of anthropology》1981,13(2):149-163
Testart's hypothesis is placed in historical perspective. The Kaiabara case study, prominent in Testart's paper but of illustrative significance rather than as proof in his book, is discussed. Testart's mathematical analysis of the degree of coherence between the classifications of societies with patrimoieties as against those with matrimoieties provides an interesting means of reaching inferences about the evolution of social organization, although the required quantification involves some difficulties. Testart's further major contribution to the understanding of moiety organization is his proposed principle of classification based on the violator/non-violator diachotomy. He shows this principle to be more consistent with matrilineal than with patrilineal classifications. It is argued that the Eagle-Crow Myth, from which Testart derives the violator-non-violator diachotomy, refers to the Oedipal conflict, and that this is related to the formation of matrilineal moieties. It is further suggested that the taboo on blood, central to the ‘violator’ concept, is connected with matrilineality. 相似文献
5.
Willy J. Peumans Hetty M. Stinissen Mieke Tierens Albert R. Carlier 《Plant cell reports》1982,1(5):212-214
Cell-free extracts from dry wheat (Triticum vulgare L.) and rye (Secale cereale L.) embryos do not synthesize their corresponding lectins when incubated under conditions optimalized for translation of either exogenous or endogenous mRNA. Only when the extracts are prepared and incubated in the complete absence of sulphydryl containing compounds lectins are synthesized in vitro. Since immunoprecipitation techniques could not demonstrate unequivocally the presence of lectin among the cell-free translation products a specific affinity purification procedure has been developed in order to proof the presence of stored lectin mRNAs in dry wheat and rye embryos.Abbreviations DTT
dithiothreitol
- SDS-PAGE
sodium dodecyl sulphate polyacrylamide gel electrophoresis
- TCA
trichloracetic acid 相似文献
6.
Ramireddy Eswarayya Nelissen Hilde Leuendorf Jan Erik Van Lijsebettens Mieke Inzé Dirk Schmülling Thomas 《Plant molecular biology》2021,106(6):555-567
Plant Molecular Biology - Root-specific expression of a cytokinin-degrading CKX gene in maize roots causes formation of a larger root system leading to higher element content in shoot organs. The... 相似文献
7.
De Bauw Pieterjan Smolders Erik Verbeeck Mieke Senthilkumar Kalimuthu Houben Eva Vandamme Elke 《Plant and Soil》2021,463(1-2):187-204
Plant and Soil - Upland rice production is often constrained by phosphorus deficiency (P) and drought events. Methods are needed to maximize P use efficiency, while promoting deep root development... 相似文献
8.
Susan Kühnast Mieke C. Louwe Mattijs M. Heemskerk Elsbet J. Pieterman Jan B. van Klinken Sjoerd A. A. van den Berg Johannes W. A. Smit Louis M. Havekes Patrick C. N. Rensen José W. A. van der Hoorn Hans M. G. Princen J. Wouter Jukema 《PloS one》2013,8(6)
Objective
Niacin potently lowers triglycerides, mildly decreases LDL-cholesterol, and largely increases HDL-cholesterol. Despite evidence for an atheroprotective effect of niacin from previous small clinical studies, the large outcome trials, AIM-HIGH and HPS2-THRIVE did not reveal additional beneficial effects of niacin (alone or in combination with laropiprant) on top of statin treatment. We aimed to address this apparent discrepancy by investigating the effects of niacin without and with simvastatin on atherosclerosis development and determine the underlying mechanisms, in APOE*3Leiden.CETP mice, a model for familial dysbetalipoproteinemia (FD).Approach and Results
Mice were fed a western-type diet containing cholesterol without or with niacin (120 mg/kg/day), simvastatin (36 mg/kg/day) or their combination for 18 weeks. Similarly as in FD patients, niacin reduced total cholesterol by -39% and triglycerides by −50%, (both P<0.001). Simvastatin and the combination reduced total cholesterol (−30%; −55%, P<0.001) where the combination revealed a greater reduction compared to simvastatin (−36%, P<0.001). Niacin decreased total cholesterol and triglycerides primarily by increasing VLDL clearance. Niacin increased HDL-cholesterol (+28%, P<0.01) and mildly increased reverse cholesterol transport. All treatments reduced monocyte adhesion to the endothelium (−46%; −47%, P<0.01; −53%, P<0.001), atherosclerotic lesion area (−78%; −49%, P<0.01; −87%, P<0.001) and severity. Compared to simvastatin, the combination increased plaque stability index [(SMC+collagen)/macrophages] (3-fold, P<0.01). Niacin and the combination reduced T cells in the aortic root (−71%, P<0.01; −81%, P<0.001). Lesion area was strongly predicted by nonHDL-cholesterol (R2 = 0.69, P<0.001) and to a much lesser extent by HDL-cholesterol (R2 = 0.20, P<0.001).Conclusion
Niacin decreases atherosclerosis development mainly by reducing nonHDL-cholesterol with modest HDL-cholesterol-raising and additional anti-inflammatory effects. The additive effect of niacin on top of simvastatin is mostly dependent on its nonHDL-cholesterol-lowering capacities. These data suggest that clinical beneficial effects of niacin are largely dependent on its ability to lower LDL-cholesterol on top of concomitant lipid-lowering therapy. 相似文献9.
Eveline Wijckmans Mieke Nys Sarah Debaveye Marijke Brams Els Pardon Katrien Willegems Daniel Bertrand Jan Steyaert Rouslan Efremov Chris Ulens 《PloS one》2016,11(3)
Cys-loop receptors are membrane spanning ligand-gated ion channels involved in fast excitatory and inhibitory neurotransmission. Three-dimensional structures of these ion channels, determined by X-ray crystallography or electron microscopy, have revealed valuable information regarding the molecular mechanisms underlying ligand recognition, channel gating and ion conductance. To extend and validate the current insights, we here present promising candidates for further structural studies. We report the biochemical and functional characterization of Cys-loop receptor homologues identified in the proteome of Alvinella pompejana, an extremophilic, polychaete annelid found in hydrothermal vents at the bottom of the Pacific Ocean. Seven homologues were selected, named Alpo1-7. Five of them, Alpo2-6, were unidentified prior to this study. Two-electrode voltage clamp experiments revealed that wild type Alpo5 and Alpo6, both sharing remarkably high sequence identity with human glycine receptor α subunits, are anion-selective channels that can be activated by glycine, GABA and taurine. Furthermore, upon expression in insect cells fluorescence size-exclusion chromatography experiments indicated that four homologues, Alpo1, Alpo4, Alpo6 and Alpo7, can be extracted out of the membrane by a wide variety of detergents while maintaining their oligomeric state. Finally, large-scale purification efforts of Alpo1, Alpo4 and Alpo6 resulted in milligram amounts of biochemically stable and monodisperse protein. Overall, our results establish the evolutionary conservation of glycine receptors in annelids and pave the way for future structural studies. 相似文献
10.
Sabrina I?igo Astrid Nagels Durand Andrés Ritter Sabine Le Gall Martin Termathe Roland Klassen Takayuki Tohge Barbara De Coninck Jelle Van Leene Rebecca De Clercq Bruno P.A. Cammue Alisdair R. Fernie Kris Gevaert Geert De Jaeger Sebastian A. Leidel Raffael Schaffrath Mieke Van Lijsebettens Laurens Pauwels Alain Goossens 《Plant physiology》2016,172(2):858-873