排序方式: 共有8条查询结果,搜索用时 31 毫秒
1
1.
Richard F. Selden Andre Steinmetz Lee McIntosh Lawrence Bogorad Gerard Burkard Mfika Mubumbila Marcel Kuntz Edwin J. Crouse Jacques H. Weil 《Plant molecular biology》1983,2(3):141-153
A minimum of 37 genes corresponding to tRNAs for 17 different amino acids have been localized on the restriction endonuclease cleavage site map of theZea mays chloroplast DNA molecule. Of these, 14 genes corresponding to tRNAs for 11 amino acids are located in the larger of the two single-copy regions which separate the two inverted copies of the repeat region. One tRNA gene is in the smaller single-copy region. Each copy of the large repeated sequence contains, in addition to the ribosomal RNA genes, 11 tRNA genes corresponding to tRNAs for 8 amino acids. The genes for tRNA2 Ile and tRNAAla map in the ribosomal spacer sequence separating the 16S and 23S ribosomal RNA genes. The three isoaccepting species for the tRNAsLeu and the three for tRNAsSer, as well as the two isoaccepting species for tRNAAsn, tRNAGly, tRNAsIle, tRNAsMet, tRNAsThr, are shown to be encoded at different loci. Two independent methods have been used for the localization of tRNA genes on the physical map of the maize chloroplast DNA molecule: (a) cloned chloroplast DNA fragments were hybridized with radioactively-labelled total 4S RNAs, the hybridized RNAs were then eluted, and identified by two-dimensional polyacrylamide gel electrophoresis, and (b) individual tRNAs were32P-labelledin vitro and hybridized to DNA fragments generated by digestion of maize chloroplast DNA with various restriction endonucleases. 相似文献
2.
3.
4.
Mfika Mubumbila Karl H.J. Gordon Edwin J. Crouse Gérard Burkard Jacques-Henry Weil 《Gene》1983,21(3):257-266
Construction of a physical map of the chloroplast DNA from Phaseolus vulgaris showed that this circular molecule is segmentally organized into four regions. Unlike other chloroplast DNAs which have analogous organization, two single-copy regions that separate two inverted repeats have been demonstrated to exist in both relative orientations, giving rise to two populations of DNA molecules.Hybridization studies using individual rRNA and tRNA species revealed the location of a set of rRNA genes and at least seven tRNA genes in each inverted repeat region, a minimum of 17 tRNA genes in the large single-copy region and one tRNA gene in the small single-copy region. The tRNA genes code for 24 tRNA species corresponding to 16 amino acids. Comparison of this gene map with those of other chloroplast DNAs suggests that DNA sequence rearrangements, involving some tRNA genes, have occurred. 相似文献
5.
Edwin J. Crouse Mfika Mubumbila Bjarn M. Stummann Gerhard Bookjans Christine Michalowski Hans J. Bohnert Jacques-Henry Weil Knud W. Henningsen 《Plant molecular biology》1986,7(2):143-149
Summary Isolated chloroplasts from Pisum sativum were found to contain at least 32 tRNA species. Hybridization of in vitro labeled, identified, chloroplast tRNAs to Pisum chloroplast DNA fragments revealed the locations of the tRNA genes on the circular chloroplast genome. Comparison of this gene map to the maps of Vicia faba and Phaseolus vulgaris showed that the chloroplast genomes of Pisum and Phaseolus are otherwise more closely related than either genome is to the chloroplast genome of Vicia. Furthermore, the results suggest how possible recombination events could be involved in the evolution of these three closely related, but divergent, chloroplast genomes. 相似文献
6.
M. Keller G. Burkard H.J. Bohnert M. Mubumbila K. Gordon A. Steinmetz D. Heiser E.J. Crouse J.H. Well 《Biochemical and biophysical research communications》1980,95(1):47-54
Hybridization studies of Euglena chloroplast 125I-labeled tRNAs to restriction fragments of Euglena chloroplast DNA have shown that the spacer between the 16S and 23S rRNA genes, in two and possibly all three of the ribosomal DNA units, contains genes for tRNAIle and tRNAAla, whereas a tRNA gene (for either tRNATrp or tRNAGlu) is located before probably all four 16S rRNA genes present on the chloroplast DNA molecule. 相似文献
7.
M. Mubumbila C. M. Bowman F. Droog T. Dyer M. Kuntz J. H. Weil 《Plant molecular biology》1985,4(5):315-320
Fractionation (by two-dimensional polyacrylamide gel electrophoresis) of total tRNA from wheat chloroplasts yields about 33
RNA spots. Of these, 30 have been identified by aminoacylation as containing tRNAs specific for 17 amino acids.
Hybridization of labeled individual tRNAs to cloned chloroplast DNA fragments has revealed the location of at least nine pairs
of tRNA genes in the segments of the inverted repeat, at least twelve tRNA genes in the large single copy region and one tRNA
gene in the small single copy region.
A comparison of this wheat chloroplast tRNA gene map to that of maize and of other higher plants suggests that gene rearrangements
have occurred during evolution, even within cereal chloroplast DNA. These rearrangements have taken place within the inverted
repeat, within the large single copy region and between the inverted repeat and the large single copy region. 相似文献
8.
Marcel Kuntz Edwin J. Crouse Mfika Mubumbila Gérard Burkard Jacques-Henry Weil Hans J. Bohnert Hernann Mucke Wolfgang Löffelhardt 《Molecular & general genetics : MGG》1984,194(3):508-512
Summary The 4S RNA of cyanelles from Cyanophora paradoxa strain LB 555 UTEX was fractionated by two-dimensional gel electrophoresis. Individual tRNA species were identified by aminoacylation, labeled in vitro and hybridized to restriction endonuclease fragments of cyanelle DNA. Hybridization experiments, using individual tRNA species, have revealed the location of two tRNA genes, coding for tRNAAla and tRNAIle, in each of the two spacer segments separating the 16S and 23S rRNA genes on the two inverted repeats (10 kbp each) and three tRNA genes in the small single-copy region (17 kbp) separating the two inverted repeats. A minimum of 14 tRNA genes in the large single-copy region (88.5 kbp) has also been found.Heterologous hybridization studies, using cyanelle tRNAs and chloroplast DNA from spinach, broad bean, or maize, indicate a high degree of homology between some tRNAs from cyanelles and chloroplasts.Although cyanelles are often condisered as having evolved from endosymbiotic cyanobacteria, the organization of tRNA genes on cyanelle DNA and the results of heterologous hybridization studies show that cyanelles are related to higher plant chloroplasts. 相似文献
1