排序方式: 共有62条查询结果,搜索用时 15 毫秒
1.
Auxin-induced mRNA species in tobacco cell cultures 总被引:6,自引:0,他引:6
E. J. van der Zaal J. Memelink A. M. Mennes A. Quint K. R. Libbenga 《Plant molecular biology》1987,10(2):145-157
2.
Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco 总被引:13,自引:0,他引:13
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Tobacco shoots exposed to elevated endogenous or exogenous cytokinin levels are unable to develop roots and lack apical dominance. We have isolated cDNA copies of five mRNA species that accumulate to elevated levels in such cytokinin-stressed shoots via differential screening of a cDNA library of transgenic shoots which contain an active T-DNA cytokinin gene (T-cyt gene) from Agrobacterium tumefaciens. Four of the cDNA clones were found to correspond to plant defence-related mRNAs, encoding extensin, chitinase, PR-1 and a PR-1-like protein, respectively. In normal tobacco plants PR-1 mRNA is relatively rare in all organs. The other four mRNAs occur at relatively low levels in shoots, especially in leaves, but are very prevalent in roots. Extensin mRNA, for example, is not detectable in leaves, while it is an abundant mRNA in roots and stems. In normal shoots cultured on cytokinin-containing medium all five mRNAs accumulate to elevated levels, similar to those found in transgenic T-cyt shoots. We conclude that the imposed cytokinin stress causes changes in the tissue-specific control of the levels of several defence-related mRNA species in tobacco. 相似文献
3.
4.
5.
6.
Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals 总被引:10,自引:0,他引:10
R. Verpoorte R. van der Heijden H.J.G. ten Hoopen J. Memelink 《Biotechnology letters》1999,21(6):467-479
The technology of large-scale plant cell culture is feasible for the industrial production of plant-derived fine chemicals. Due to low or no productivity of the desired compounds the economy is only in a few cases favorable. Various approaches are studied to increase yields, these encompass screening and selection of high producing cell lines, media optimization, elicitation, culturing of differentiated cells (organ cultures), immobilization. In recent years metabolic engineering has opened a new promising perspectives for improved production in a plant or plant cell culture. 相似文献
7.
8.
Rafael Zárate Johan Memelink Robert van der Heijden Robert Verpoorte 《Biotechnology letters》1999,21(11):997-1002
In vitro propagation of Catharanthus roseus was achieved using nodal explants. Bud induction was best on medium containing 1.0 mg benzyl aminopurine l–1. Hardening of rooted shoots to soil was very successful with 98% survival. Genetically transformed C. roseus plantlets were obtained after bombardment of nodal explants, which were then micropropagated, with DNA coated particles with green fluorescent protein (GFP) or -glucuronidase (GUS) reporter genes. Histological studies showed that the gene insertion method proved effective with many cells and different tissues displaying the reporter gene signals, showing that gene expressions were rather stable. 相似文献
9.
10.
Chatel G Montiel G Pré M Memelink J Thiersault M Saint-Pierre B Doireau P Gantet P 《Journal of experimental botany》2003,54(392):2587-2588