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1.
Phosphorylation of translation elongation factor 2(eEF-2) by a specific Ca2+/calmodulin-dependent eEF-2 kinase plays an important role in the regulation of protein synthesis in mammalian cells. We show here that an eEF-2 kinase similar to the mammalian enzyme is present in tissues of the amphibian Xenopus laevis. We investigated changes in the activity of eEF-2 kinase in extracts of Xenopus oocytes at different stages of oogenesis. The eEF-2 kinase activity was constant from stage I to stage IV of oogenesis, but dramatically decreased after stage IV. Extracts of fully grown stage-VI oocytes showed no eEF-2 kinase activity. However, when extracts were analyzed by two-dimensional gel electrophoresis, eEF-2 was found to be present mostly, if not exclusively, in the dephosphorylated form throughout oogenesis. It is suggested that eEF-2 kinase disappears late in oogenesis to make protein synthesis insensitive to changes in intracellular Ca2+ concentration. This may be important for the induction of meiotic maturation. 相似文献
2.
DNA-like duplexes with repetitions. III. Efficient template-guided chemical polymerization of d(TGGCCAAGCTp). 总被引:8,自引:8,他引:0
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Z A Shabarova N G Dolinnaya V L Drutsa N P Melnikova A A Purmal 《Nucleic acids research》1981,9(21):5747-5761
Self-association of a decanucleotide d(TGGCCAAGCTp) in an aqueous solution is shown by UV spectroscopy, CD and sedimentation analysis to yield a pseudopolymeric (concatemeric) duplex having a geometry similar to that of DNA B-type. It is demonstrated that in conditions when the concatemeric duplex is stable a water-soluble carbodiimide induces efficient polymerization of the 3'- or 5'-phosphorylated decanucleotide, and the resulting polymers d(TGGCCAAGCTp)2-10 contain only natural phosphodiester bonds. In conditions optimal for template-guided polymerization of d(TGGCCAAGCTp) the overall yield of 20-100-member polynucleotides exceeds 90%. The obtained polymeric duplexes are cleaved by restriction endonuclease Alu II, Bsu RI, and Hind III to corresponding decamers which were isolated and sequenced. 相似文献
3.
A. F. Melnikova A. S. Zasedatelev A. M. Kolchinsky G. V. Gursky A. L. Zhuze S. L. Grochovsky A. D. Mirzabekov 《Molecular biology reports》1975,2(2):135-142
The interaction of the antibiotics distamycin A, distamycin analogue and netropsin with chromatin of calf thymus has been studied by circular dichroism measurements and by gel filtration. The minor groove of DNA in chromatin is accessible by 83–89% to the binding of these antibiotics as compared with that of free DNA. The present results combined with our data on the methylation of chromatin with dimethylsulphate [3] strongly suggest that the minor groove of DNA in chromatin is not occupied by chromatin proteins.Abbreviations DM
distamycin A
- DM2
analogue of distamycin
- Nt
netropsin
- CD spectra
circular dichroism spectra 相似文献
4.
5.
Melnikova O. V. Vershinin E. A. Verzhutskaya Yu. A. Korzun V. M. Adelshin R. V. Trushina Yu. N. Andaev E. I. 《Entomological Review》2021,101(9):1471-1484
Entomological Review - The state of the natural foci of tick-borne encephalitis (TBE) was monitored during 15 seasons (2005–2019) in several key plots within a popular recreational and... 相似文献
6.
7.
Paleontological Journal - Twelve new species of Ordovician ostracodes are described from the sections on the Moyero, Angara and Nizhnyaya Chunku rivers. They belong to six previously described... 相似文献
8.
A. K. Golovnin V. V. Molodina P. G. Georgiev L. S. Melnikova 《Doklady. Biochemistry and biophysics》2016,469(1):247-252
This study is devoted to clarifying the role of Mod(mdg4)-67.2 and Su(Hw) proteins in the interaction between Su(Hw)-dependent insulator complexes and identifying the specific domains of the Su(Hw) protein required for insulation or mutual neutralization of insulators. Using genetic techniques and experiments in yeast two-hybrid system, we have demonstrated that the zinc finger domain of the Su(Hw) protein is involved in forming a functional insulator complex and cannot be replaced with the DNA-binding domain of the GAL4 protein. 相似文献
9.
N. V. Koroban A. V. Kudryavtseva G. S. Krasnov A. F. Sadritdinova M. S. Fedorova A. V. Snezhkina N. L. Bolsheva O. V. Muravenko A. A. Dmitriev N. V. Melnikova 《Molecular Biology》2016,50(3):337-343
Regulation of gene expression via microRNA is the key mechanism of response to biotic and abiotic stresses in plants. There are a lot of experimental data on the biological function of microRNAs in response to different stresses in various plant species. This review contains up-to-date information on molecular mechanisms of microRNA action in plants in response to abiotic stresses, including drought, salinity, mineral nutrient deficiency or imbalance. 相似文献
10.
Dmitriev Alexey A. Kudryavtseva Anna V. Krasnov George S. Koroban Nadezhda V. Speranskaya Anna S. Krinitsina Anastasia A. Belenikin Maxim S. Snezhkina Anastasiya V. Sadritdinova Asiya F. Kishlyan Natalya V. Rozhmina Tatiana A. Yurkevich Olga Yu. Muravenko Olga V. Bolsheva Nadezhda L. Melnikova Nataliya V. 《BMC plant biology》2016,16(3):139-146