全文获取类型
收费全文 | 4266篇 |
免费 | 421篇 |
国内免费 | 1篇 |
出版年
2024年 | 6篇 |
2023年 | 13篇 |
2022年 | 59篇 |
2021年 | 89篇 |
2020年 | 59篇 |
2019年 | 54篇 |
2018年 | 87篇 |
2017年 | 90篇 |
2016年 | 146篇 |
2015年 | 253篇 |
2014年 | 254篇 |
2013年 | 294篇 |
2012年 | 369篇 |
2011年 | 391篇 |
2010年 | 243篇 |
2009年 | 205篇 |
2008年 | 293篇 |
2007年 | 283篇 |
2006年 | 260篇 |
2005年 | 188篇 |
2004年 | 179篇 |
2003年 | 182篇 |
2002年 | 165篇 |
2001年 | 39篇 |
2000年 | 41篇 |
1999年 | 46篇 |
1998年 | 36篇 |
1997年 | 30篇 |
1996年 | 22篇 |
1995年 | 30篇 |
1994年 | 28篇 |
1993年 | 22篇 |
1992年 | 32篇 |
1991年 | 19篇 |
1990年 | 16篇 |
1989年 | 14篇 |
1988年 | 17篇 |
1987年 | 14篇 |
1986年 | 13篇 |
1985年 | 7篇 |
1984年 | 12篇 |
1983年 | 9篇 |
1982年 | 11篇 |
1981年 | 5篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1976年 | 7篇 |
1974年 | 4篇 |
1973年 | 6篇 |
1972年 | 4篇 |
排序方式: 共有4688条查询结果,搜索用时 15 毫秒
1.
Inflammatory reaction to ocular trauma is known to be related to prostaglandins. To further evaluate this phenomenon in human, PGE1 and E2 were measured by R.I.A. after silicic acid chromatography in aqueous humour of 26 patients before and 3 days after surgery for unilateral senile cataract (posterior chamber implant after extracapsular surgery). 13 out of these patients were treated with indomethacin by enteral route and 13 were not treated.PGE2 levels increased in all non-treated patients from : m < 214 pg/ml before surgery, to 2666 ± 869 pg/ml (range : 257 - 8728) p < 0.001 after surgery. PGE2 levels did not increase in indomethacin-treated patients. PGE1 levels did not increase significantly in non-treated as in treated patients.1) Intra-ocular surgery is followed in human by a constant increase of the only PGE2 in the aqueous humour. 2) Indomethacin inhibits this increase. 3) The post-surgical increase in the permeability of blood-aqueous barrier appears to be related to a release of PGE2. 相似文献
2.
3.
4.
The precise regulation of synapse maintenance is critical to the development and function of neuronal circuits. Using an in vivo RNAi screen targeting the Drosophila kinome and phosphatome, we identify 11 kinases and phosphatases controlling synapse stability by regulating cytoskeletal, phospholipid, or metabolic signaling. We focus on casein kinase 2 (CK2) and demonstrate that the regulatory (β) and catalytic (α) subunits of CK2 are essential for synapse maintenance. CK2α kinase activity is required in the presynaptic motoneuron, and its interaction with CK2β, mediated cooperatively by two N-terminal residues of CK2α, is essential for CK2 holoenzyme complex stability and function in vivo. Using genetic and biochemical approaches we identify Ankyrin2 as a key presynaptic target of CK2 to maintain synapse stability. In addition, CK2 activity controls the subcellular organization of individual synaptic release sites within the presynaptic nerve terminal. Our study identifies phosphorylation of structural synaptic components as a compelling mechanism to actively control the development and longevity of synaptic connections. 相似文献
5.
Patients with primary polycythaemia may present clinical features which lead to an erroneous diagnosis of intracerebral neoplasm. Three such cases are described in detail in this report. 相似文献
6.
7.
8.
Aim Species capable of vigorous growth under a wide range of environmental conditions should have a higher chance of becoming invasive after introduction into new regions. High performance across environments can be achieved either by constitutively expressed traits that allow for high resource uptake under different environmental conditions or by adaptive plasticity of traits. Here we test whether invasive and non‐invasive species differ in presumably adaptive plasticity. Location Europe (for native species); the rest of the world and North America in particular (for alien species). Methods We selected 14 congeneric pairs of European herbaceous species that have all been introduced elsewhere. One species of each pair is highly invasive elsewhere in the world, particularly so in North America, whereas the other species has not become invasive or has spread only to a limited degree. We grew native plant material of the 28 species under shaded and non‐shaded conditions in a common garden experiment, and measured biomass production and morphological traits that are frequently related to shade tolerance and avoidance. Results Invasive species had higher shoot–root ratios, tended to have longer leaf‐blades, and produced more biomass than congeneric non‐invasive species both under shaded and non‐shaded conditions. Plants responded to shading by increasing shoot–root ratios and specific leaf area. Surprisingly, these shade‐induced responses, which are widely considered to be adaptive, did not differ between invasive and non‐invasive species. Main conclusions We conclude that high biomass production across different light environments pre‐adapts species to become invasive, and that this is not mediated by plasticities of the morphological traits that we measured. 相似文献
9.
During growth on glycerol two marine Desulfovibrio strains that can grow on an unusually broad range of substrates contained high activities of glycerol kinase, NAD(P)-independent glycerol 3-phosphate dehydrogenase and the other enzymes necessary for the conversion of dihydroxyacetone phosphate to pyruvate. Glycerol dehydrogenase and a specific dihydroxyacetone kinase were absent. During growth on dihydroxyacetone, glycerol kinase is involved in the initial conversion of this compound to dihydroxyacetone phosphate which is then further metabolized. Some kinetic properties of the partially purified glycerol kinase were determined. The role of NAD as electron carrier in the energy metabolism during growth of these strains on glycerol and dihydroxyacetone is discussed.Glycerol also supported growth of three out of four classical Desulfovibrio strains tested. D. vulgaris strain Hildenborough grew slowly on glycerol and contained glycerol kinase, glycerol 3-phosphate dehydrogenase and enzymes for the dissimilation of dihydroxyacetone phosphate. In D. gigas which did not grow on glycerol the enzymes glycerol kinase and glycerol 3-phosphate dehydrogenase were absent in lactate-grown cells.Abbreviations DHA
dihydroxyacetone
- DHAP
dihydroxyacetone phosphate
- G3P
glycerol 3-phosphate
- GAP
glyceraldehyde 3-phosphate
- 3-PGA
3-phosphoglycerate
- 2-PGA
2-phosphoglycerate
- 2,3-DPGA
2,3-diphosphoglycerate
- PEP
phosphoenolpyruvate
- DH
dehydrogenase
- GK
glycerol kinase
- DHAK
dihydroxyacetone kinase
- TIM
triosephosphate isomerase
- PGK
3-phosphoglycerate kinase
- PK
pyruvate kinase
- LDH
lactate dehydrogenase
- DTT
dithiotreitol
- HEPES
4-(2-hydroxyethyl)-1-piperazine ethane sulfonic acid
- PIPES
piperazine-1,1-bis(2-ethane sulfonic acid)
- BV2+/BV+
oxidized/reduced benzylviologen
- PMS
phenazine methosulfate
- DCPIP
2,6-dichlorophenolindophenol
- MTT
3-(4,5-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide 相似文献
10.
Our goal was to test our understanding of ingestion, assimilationefficiency and metabolism for Mnemiopsis mccradyi by formulatingand validating a simulation model of growth under differentconditions of food availability. The model was based on a carbonbudget approach using formulations derived from empirical results,including how each process was affected by food availabilityand ctenophore size. An experimentally measured carbon budgetfor pulsed food availability indicated that, relative to totalingestion, growth was high (1748%), respiration plusorganic release was relatively low (2448%) and little(<10%) of the ingested carbon was unaccounted for. New laboratoryinvestigations of feeding and assimilation efficiency were necessaryto refine the formulations so that model predictions comparedfavorably with a variety of laboratory measurements of growth,and growth efficiency, as well as the complete experimentallymeasured carbon budget. The refined model predicted a high ratioof growth to metabolism (>2) and a high gross growth efficiency(>30%) for smaller ctenophores at high food concentrations(>20 prey l1). Both growth rates and growth efficiencieswere predicted to decrease for larger ctenophores. Model predictionswere generally consistent with experimental results, includinginvestigations using pulsed food availability to simulate environmentalpatchiness. Although the model underpredicted ctenophore growthin some experiments at low food densities, the model predictionof a minimum prey concentration of about 8 l1 (24 µgC l1) for sustaining a ctenophore population of reproductivesize agreed with field observations. 相似文献