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Mehbub Mohammad F. Tanner Jason E. Barnett Stephen J. Franco Christopher M. M. Zhang Wei 《Applied microbiology and biotechnology》2016,100(24):10609-10626
Applied Microbiology and Biotechnology - Sponge-associated bacteria play a critical role in sponge biology, metabolism and ecology, but how they interact with their host sponges and the role of... 相似文献
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Aniruddha Saha Mehbub Isha Sourish Dasgupta Dipanwita Saha 《Archives Of Phytopathology And Plant Protection》2013,46(18):1781-1795
Pathogenicity test of the fungal pathogen Colletotrichum gloeosporioides (Penz.) Sacc., causal agent for anthracnose in eggplant (Solanum melongena L.), was performed in 28 commercially cultivated eggplant varieties by analysing the antigenic patterns of host and pathogen. Through initial screening following detached leaf inoculation technique and whole plant inoculation technique, Pusa purple long (Ppl) variety was found to be the most susceptible while Shamala variety (Shav) was the most resistant. Cross-reactive antigens (CRA) shared by susceptible varieties and C. gloeosporioides was detected by immunodiffusion and immunoelectrophoresis and indirect enzyme-linked immunosorbent assay (ELISA). Such antigens could not be detected between the resistant varieties and the pathogen and also between a nonpathogen (Alternaria porri) and all the test varieties. However, ELISA showed that low levels of common antigens were present between all combinations. The level of CRA was found to decrease with increasing resistance. Indirect immunogold labeling followed by silver enhancement revealed that CRA were concentrated mainly in the cell wall regions throughout the tissue. The level of CRA was found to correlate to the pathogenicity of C. gloeosporioides in different eggplant varieties. ELISA may therefore be used to screen the commercially cultivated eggplant varieties for resistance to C. gloeosporioides. 相似文献
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