Characterization of murine cell lines from Diethylstilbestrol-Induced uterine endometrial Adenocarcinomas

Neonatal treatment with estrogens is associated with development of uterine adenocarcinomas in CD-1 mice. Treatment with the synthetic estrogen diethylstilbestrol (DES) on Days 1 to 5 after birth results in 90% incidence of these hormonedependent lesions in 18-mo.-old mice. Three cell lines were established from these DES-associated tumors. Each of these cell lines exhibited morphologic and ultrastructural characteristics of transformed epithelial cells, including an increased nuclear:ytoplasmic ratio, enlarged and irregular nuclei with multiple nucleoli and areas of chromatin condensation, positive staining for cytokeratin, desmosomes, and microvilli. After subcutaneous injection into nude mice, all three cell lines formed solid tumors within 4 wk. Although the primary uterine tumors and tumor transplants in nude mice had been shown to be estrogen-dependent and estrogen-receptor positive, neither the monolayer growth nor the tumorigenicity of any of the three cell lines in this study was enhanced by or dependent on estrogen. Estrogen receptor levels were low in early and intermediate passage cells. Allele-specific oligonucleotide hybridization analysis of PCR-amplified cell line DNA revealed no point mutations in the 12th, 13th, or 61st codons of the K-ras or H-ras protooncogenes. Southern analysis revealed no changes in genomic organization of the putative tumor suppressor gene DCC, but demonstrated a three-to four-fold amplification of the c-myc gene in one cell line. Expression of c-myc RNA was concomitantly increased in the same cell line. These three transformed cell lines represent the end point in the process of hormone-associated tumorigenesis and as such should prove useful in investigating the molecular changes and the mechanisms involved in hormonal carcinogenesis.     

HABITAT USE AND PREFERENCES OF INDO-PACIFIC HUMPBACK DOLPHINS SOUSA CHINENSIS IN ALGOA BAY, SOUTH AFRICA

This paper examines environmental and behavioral determinants of the habitat use and preferences of Indo-Pacific humpback dolphins inhabiting the Algoa Bay region on the south Eastern Cape coast of South Africa. In order to quantify the habitat use and preference, two indices were used, the Coefficient of Area Use (AU) and the Activity Index (AI). The dolphins inhabit a narrow strip of shallow, inshore waters of Algoa Bay and remain mostly within 400 m of the shore, in water less than 15 m deep, with no apparent preference for clear or turbid water. Water depth is probably the main factor limiting their inshore distribution, and the 25-m isobath seems to represent the critical depth. Within this confined, inshore distribution, dolphin activities concentrate in the vicinity of rocky reefs-their primary feeding grounds. Dolphin dependence on these shallow-water habitats is evident throughout the year and, consequently, the inshore shallow reefs are identified as the "key habitat" which is of primary importance for humpback dolphins in Eastern Cape waters. The dolphins' dependence on this restricted type of habitat within an already restricted inshore distribution makes them particularly vulnerable to alteration or loss of this habitat.     

Swarming behavior in male chironomid midges: a cost-benefit analysis

Aerial mating swarms of nonbiting male midges form at dusk andattract females from the surrounding vegetation. Females flyinto the swarm, and copulation occurs on the wing. Mating andpredation are identified as the major benefit and cost of swarmingand are influenced by swarm size in opposing ways. Swarms varygreatly in size but the individual's probability of mating isgreatest in the smallest swarms. However, the individual predationrisk is also greatest in the smallest swarms. These opposingeffects on swarm size combine in a common currency of matingsuccess per evening to favor males in the smallest swarms. Thereis also an effect of male body size. The smallest males occurpredominantly in the smallest swarms and have the highest matingsuccess. The mechanisms that might maintain the observed swarmsize distribution are discussed.     

Depth-related variation in gross chemical composition of several seaweeds

The percentages of dry weigth, ash, carbon and nitrogen in Chondrus crispus Stackh. and Furcellaria lumbricalis (Huds.) Lamour. were determined for plants distributed along a depth gradient. Analyses were made of samples collected from June throug August. Dry weights and ash were also determined for other major perennial species, but on fewer samples. In all instances the trend was an increase in ash as a percentage of the dry weight and a decrease in the percentage dry weight and organic matter, with depth. The percentage of carbon also decreased with depth, reflecting the changes in organic matter. However, the percentage of nitrogen increased with depth, thus the C/N ratio decreased with depth. These changes in gross chemical composition are assumed to be analogous to seasonal changes in composition at a given depth or level in the littoral zone.     

Rain-pools on peat moorland as island habitats for midge larvae

A common aquatic habitat on peat moorland is a pool, often only a few centimetres deep. Many have a shorter average duration than that of the inhabitants but they are, nonetheless, inhabited by the larval stages of several chironomid species at moderately high densities. Because of their durational characteristics, these pools might be expected to have an endemic fauna, as is the case in similar ephemeral island habitats elsewhere. However, we find no such fauna. Indeed, we find no qualitative difference between them and permanent pools of the same substratum.We identify three reasons for the apparent absence of endemism. First, the midge fauna is able to survive dry periods in the moist mud, so that even the smallest pools are effectively permanent from the adaptive point of view. Second, although seasonal freezing eliminates the fauna of smaller pools, larvae are able to complete their development in the preceding summer. Third, nearby permanent pools which do not dry and in which the mud does not freeze, may act as refugia for re-invasion.     

POPULATION ANALYSES OF INDO-PACIFIC HUMPBACK DOLPHINS SOUSA CHINENSIS IN ALGOA BAY,EASTERN CAPE,SOUTH AFRICA1

Mark-recapture analyses were performed on photo-identification data for Indo-Pacific humpback dolphins (Sousa chinensis) inhabiting Algoa Bay, on the Eastern Cape coast of South Africa. Boat-based photo-ID surveys were undertaken between May 1991 and May 1994. The rate of discovery of newly identified dolphins, distribution of sightings and frequency of resightings of known individuals indicate a high level of seasonal immigration of humpback dolphins into, and emigration from, the Algoa Bay region in summer. Consequently, humpback dolphins from Algoa Bay appear to be part of a substantially larger population that uses a considerable length of the coastal zone. The minimum population size is estimated to be about 466 dolphins.     

Tidal movements of the macrofauna on an exposed sandy beach in South Africa

All epifaunal and shallow-burrowing, intertidal macrofauna on an exposed sandy beach appear to undergo tidal migrations which vary in extent but are all upshore on the incoming tide and downshore on the outgoing tide during day and night. In the crustaceans this is probably endogenous rhythmic behaviour and they show increased activity at night. In the molluscs this movement is not synchronised by an intrinsic mechanism but results from behavioural responses to changing physical conditions. The large wedge clam, Donax serra, shows no tidal migrations but does show a semilunar rhythm of movement from just above mean tide level (MTL) during springs to the low tide level during neap tides.