排序方式: 共有33条查询结果,搜索用时 130 毫秒
1.
Agrobacterium tumefaciens carrying a disarmed Ti-plasmid vector containing a chimeric NPT-II gene and a glyphosate resistance plant-derived 5-enolpyruvylshikimate-3-phosphate synthase gene was used to transform flax hypocotyl tissues. Transformed shoots could be regenerated from the inoculated tissue and were proven to be transgenic by the combination of leaf callus assays, nopaline assays and progeny tests. Co-segregation was observed in the progeny for kanamycin and glyphosate resistance. 相似文献
2.
Until recently, grain legumes in general have proven recalcitrant at de novo regeneration in vitro. By culturing portions of lentil (Lens culinaris) shoot meristems and epicotyls on a medium containing kinetin and gibberellic acid, callus tissue was produced which could be induced to regenerate shoots in relatively large numbers, even after several subcultures. The shoots could be rooted in a mist chamber to yield whole, fertile plants. 相似文献
3.
Summary Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while A281 produced the heaviest tumors in vitro. Tumor formation and opine production are indicative of plant cell transformation and tumors produced appropriate opines: nopaline (C58), octopine (Ach5 and GV3111), and agropine and mannopine (A281). Southern analysis of DNA from a tumor line produced by strain C58 showed that a T-DNA fragment had been transferred into the lentil genome. 相似文献
4.
5.
6.
Fatal flaws in agbiotech regulatory policies 总被引:2,自引:0,他引:2
McHughen A 《Nature biotechnology》2007,25(7):725-727
7.
8.
Anju Gulati Pat Schryer Alan McHughen 《In vitro cellular & developmental biology. Plant》2001,37(6):798-802
Summary A protocol for regeneration and micrografting of shoots of lentil (Lens culinaris Medik) was developed. Multiple shoots (4–5) were regenerated from cotyledonary node explants on Murashige and Skoog (MS)
medium containing 8.8 μM 6-benzylaminopurine. In vitro regenerated shoots were micrografted on rootstocks with 96% efficiency. The successful grafts were transplanted to pots in
Redi-earthTM, hardened off and were grown to maturity with 100% success. The success of the micrografting was independent of the nature
and concentration of growth regulator used in shoot initiation medium and the time period for induction of shoots. The protocol
was successful with several cultivars of lentil. The advantages of micrografting over in vitro rooting are discussed. 相似文献
9.
Summary Linseed flax (Linum usitatissimum L.) was transformed by bombarding hypocotyl tissues with gold particles coated with plasmid DNA carrying the β-glucuronidase
(GUS) (uid-A) and neomycin phosphotransferase II (npt-II) genes. Transient expression of the introduced β-glucuronidase gene was used to study factors influencing the DNA delivery,
while progeny analyses confirmed stable transformation. The efficiency of DNA delivery, uptake and expression was significantly
affected by the duration of hypocotyl preculture, bombardment distances, the level of chamber vacuum, the quantity of DNA,
and the size of particles. Nineteen independent GUS-positive shoots were recovered and regenerated into whole plants, from
which 10 plants successfully produced viable seeds. Analysis of T1 and T2 self pollinated progeny for histochemical and fluorometric GUS assays and polymerase chain reaction (PCR) analyses for uid-A, plus npt-II PCR and germination assays in progeny plants demonstrated that the transgenes were expressed in selected plants and transmitted
to progeny, usually via a single Mendelian locus. The results show that particle bombardment can be used to produce transgenic
Linum plants. The system is rapid, simple and offers an alternative to Agrobacterium methods. 相似文献
10.
A. McHughen 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,74(6):727-732
Summary Progeny of a flax (Linum usitatissimum L. cv McGregor) plant, regenerated from a cell line selected in vitro for salt tolerance (designated STS-II) was tested for its performance over two generations in normal and in saline soil against its parent variety. Germination, seedling height, flowering, seed set and seed yield in controlled greenhouse conditions were recorded. The putative salt tolerant line was superior in saline soil for all parameters measured, indicating that the mechanism selected in cells in vitro was also active in whole plants, and that the trait is genetically stable and seed transmitted. Unexpectedly, the STS-II line was also superior in the normal, non-stressed soil, indicating that the selected trait is not limited to salt tolerance specifically, suggesting a more general mechanism, such as a general increase in vigor. 相似文献