排序方式: 共有22条查询结果,搜索用时 15 毫秒
1.
Ovsova LM Mazrukho AB Lomov IuM Rozhkov KK Monakhova EK Mikhas' NK Kaminskiĭ DI Kruglikov VD 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(3):16-21
The influence of amino acids and ammonium salts on the production of cholera enterotoxin (CT) by 3 Vibrio cholerae strains of different biovars and serogroups was evaluated. As revealed in this study, toxin formation in each of the strains was quantitatively and qualitatively determined by their individual sets of amino acids. The amino acid compositions ensuring the maximum production of CT by the V. cholerae strains under study were formed. The use of ammonium salts as the only source of nitrogen in the composition of a synthetic nutrient medium for the accumulation of CT was shown to be inexpedient. 相似文献
2.
Onishchenko GG Aĭdinov GT Moskvitina EA Lomov IuM Tikhonov NG Prometnoĭ VI Shvager MM Ryzhkov VIu Savchenko PP Dmitrieva TA Batashev VV Pukhov IuM Pichurina NL Ivanova NG Gavrinev SA Kovalev EV Kipaĭkin VA Pauk VL Emel'ianova ZN Orekhov IV Lipkovich AD Stakheev VV Trepel' VG Usatkin AV Markov VI Borisevich IV Merkulov VA Makhlaĭ AA Vasil'ev NT Mishan'kin BN Vodop'ianov SO Mazrukho TV Badunenko VP 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(2):36-42
The results of the epidemiological analysis of the outbreak of hemorrhagic fever which was caused by Crimean-Congo hemorrhagic fever virus and occurred during the period of July 3-19, 1999, in the Oblivskaya district of Rostov Province are presented. The specific epidemiological features of the outbreak have been determined. The possible versions of the appearance of the focus of infection and the role of Ixodes ticks in the circulation of the infective agent are discussed. 相似文献
3.
Melanoma is the most lethal cutaneous cancer with a highly aggressive and metastatic phenotype. While recent genetic and epigenetic studies have shed new insights into the mechanism of melanoma development, the involvement of regulatory non‐coding RNAs remain unclear. Long non‐coding RNAs (lncRNAs) are a group of endogenous non‐protein‐coding RNAs with the capacity to regulate gene expression at multiple levels. Recent evidences have shown that lncRNAs can regulate many cellular processes, such as cell proliferation, differentiation, migration and invasion. In the melanoma, deregulation of a number of lncRNAs, such as HOTAIR, MALAT1, BANCR, ANRIL, SPRY‐IT1 and SAMMSON, have been reported. Our review summarizes the functional role of lncRNAs in melanoma and their potential clinical application for diagnosis, prognostication and treatment. 相似文献
4.
Monakhova EV Mironova AV Alekseeva LP Mazrukho AB 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2008,(4):27-32
AIM: Complex assessment of virulence of cholera vibrios carrying the truncated CTX element (pre-CTXphi prophage). MATERIALS AND METHODS: Twenty-two strainsof Vibriocholerae O1 and non-O1/non-O139 were studied by PCR and laboratory models. RESULTS: Genomes of all strains, besides pre-CTXphi genes, contained genes hapA (hemagglutinin/proteases), cef (CHO cell elongating factor), rtxA (high-molecular cytotoxin), and rtxC (its activator). Nucleotide sequences of rtxA and vgrG genes from ACD domains, genes VPI and VPI-2 from islands of pathogenicity, mshA (mannose-sensitive pili) gene were presented in different combinations. None strains contained shiga-like toxin (slt1) aswell as thermostable direct (tdh) and thermostable direct-related (trh) hemolysin genes of V. parahaemoliticus. On the model of infant rabbits almost all strains caused a significant enteropathogenic effect sometimes resembling cholera effect and in a number of cases dissemination of bacteria into various organs and tissues took place. Cultural supernatants of the majority of strains stipulated cell rounding in CHO cultures (one of them caused cell destruction) and disconnection of cells in McCoy and L-929 dense monolayers as well as increase of skin permeability in Craig's test. Conclusion. Apparently, diarrhea of different severity observed in patients from whom these strains were isolated as well as signs of virulence revealed in the laboratory models were determined by the expression of genes of accessory pathogenicity factors including those detected in the present study. 相似文献
5.
Priscila R Moreira Marcos A Maioli Hyllana CD Medeiros Marieli Guelfi Flávia TV Pereira Fábio E Mingatto 《Biological research》2014,47(1)
Background
The liver is an important organ for its ability to transform xenobiotics, making the liver tissue a prime target for toxic substances. The carotenoid bixin present in annatto is an antioxidant that can protect cells and tissues against the deleterious effects of free radicals. In this study, we evaluated the protective effect of bixin on liver damage induced by carbon tetrachloride (CCl4) in rats.Results
The animals were divided into four groups with six rats in each group. CCl4 (0.125 mL kg-1 body wt.) was injected intraperitoneally, and bixin (5.0 mg kg-1 body wt.) was given by gavage 7 days before the CCl4 injection. Bixin prevented the liver damage caused by CCl4, as noted by the significant decrease in serum aminotransferases release. Bixin protected the liver against the oxidizing effects of CCl4 by preventing a decrease in glutathione reductase activity and the levels of reduced glutathione and NADPH. The peroxidation of membrane lipids and histopathological damage of the liver was significantly prevented by bixin treatment.Conclusion
Therefore, we can conclude that the protective effect of bixin against hepatotoxicity induced by CCl4 is related to the antioxidant activity of the compound. 相似文献6.
Mazrukho BL Kruglikov VD Ishina EV Avdeeva EP Mazrukho AB Vodop'ianov SO Kudriakova TA Shestialtynova IS Lobanov VV Kachkina GV Kaminskiĭ DI Shut'ko AG Dudina NA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2003,(2):12-16
The dynamics of the isolation of V. cholerae cultures from various water objects on the territory of Rostov-on-Don during the period of 1994-2001 was analyzed and biological properties of 14 such cultures were studied. In the absence of epidemic complications during the above-mentioned period, a growth in the amount of V. cholerae isolates, serogroups 01 and 0139, including toxigenic V. cholerae 01, was registered. The microbiological and epidemiological aspects of the monitoring of surface reservoirs and sewage were considered and the expediency of the profound and systematic study of its results for epidemiological surveillance on cholera was emphasized. 相似文献
7.
Men'shikova EA Mironova AV Podosinnikova LS Mazrukho AB Sokolenko AV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2004,(3):78-81
The hemolytic activity of ctx- and ctx+ V. cholerae, serogroups eltor and O39, in a medium free of FeCl3 was studied. During the cultivation in this medium, the strains of both V. cholerae serogroups proved to be capable of lysing sheep red blood cells in the Graig test, irrespective of the presence of ctx genes. The cultivation of V. cholerae ctx+ strains of both serogroups under such conditions facilitated the production of hemolysin with the same spectrum of lytic activity as hemolysin produced by ctx- strains. 相似文献
8.
Avdeeva EP Mazrukho BL Voronezhskaia LG Monakhova EV Kudriakova TA Tsedova EG Ne'matov AS Inogamova IA Iakubiva IS 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2005,(3):80-82
The results of the serotyping of 244 V. cholerae non O1/O139 cultures isolated from patients in Uzbekistan in 2000 and 2001 are presented. All isolates were studied by the method of molecular probing and in the polymerase chain reaction for the presence of virulence genes and for sensitivity to phages ctx+, ctx- and hemolytic activity. The use of monoreceptor O-sera O2-O83 made it possible to determine vibrios of 32 serogroups with the dominating role in the etiology of acute enteric diseases belonging to serogroups O18, O62, O82, O37. Genes ctx AB were detected in none of the isolates, 5 of them contained gene tcp A. A group of cultures, sensitive to phage ctx+ and belonging mainly to enteropathogenic serogroups, was detected. 相似文献
9.
Avdeeva EP Mazrukho BL Ishina EV Voronezhskaia LG 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2001,(4):75-78
The development of a method for serological identification of toxigenic and nontoxigenic V. cholerae non-O1, as well as on their role in human pathology, is reviewed. The evaluation on this method when used for establishing the etiology of acute diarrhea cases and analysis of sporadic diseases and group outbreaks of alimentary toxicoinfection type. Different points of view on the formation of toxigenic clones of V. cholerae non-O1 in nature is considered. The necessity for further development of the serological typing method is substantiated and the tasks of its improvement are set. 相似文献
10.
Bardykh ID Krylikov VD Mazrukho BL Ryzhko IV Moskvitina EA Tsuraeva RI Vinokur NI Drobotkovskaia NV 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2001,(2):68-71
Investigations on experimental models of cholera ("sealed" mice and suckling rabbits) demonstrated that previous daily oral administration of the ferment culture of Lactobacillus acidophilus BKM B-2020[symbol: see text] in a dose of 3.0 x 10(8) microbial cells/ml daily for 5-7 days prevented to the development of Vibrio cholerae infection. The curative effect observed after 3 administrations of lactobacilli within 48 hours after infection with V. cholerae was registered in 50% of cases. This strain of lactobacilli was found to be suitable for use as the basis component of probiotic, an additional remedy for the prophylaxis and treatment of cholera. 相似文献