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排序方式: 共有220条查询结果,搜索用时 15 毫秒
1.
Olga Maslova Oleg Fedevych Nadiia Shuvalova Olena Deryabina Volodymyr Zhovnir Miroslav Novak Peter Kruzliak 《Cell and tissue banking》2016,17(2):335-339
The need for selection of the optimal material for the manufacturing of cardio-patches can be resolved by the use of cryostored autologous pericardial tissue. This short communication is a concise fragment of a large-scale research and demonstrates only the efficiency of cell culturing before and after pericardial preservation in the low temperature conditions. 相似文献
2.
G Iu Lipskaia O K Kutitova E V Fel'dman V I Votiakov S V Maslova 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1987,(10):30-37
Oligonucleotide mapping of the RNA genome was applied for analysis of poliovirus clinical isolates in Byelorussia in 1967-1985. All the maps have been clearly shown to resemble the reference maps of the corresponding vaccine strains. Examination of the oligonucleotide maps of poliovirus isolates provides a measure of distribution of some of the mutations over the viral genome. 相似文献
3.
E A Lesnik R N Maslova I M Agranovich 《Journal of biomolecular structure & dynamics》1987,5(3):601-614
We have determined the 1H----3H exchange rate constants between water and C8H groups of purinic residues of alternating polynucleotides poly(dA-dT).poly(dA-dT), poly(dG-dC).poly(dG-dC) and poly(dA-dC).poly(dG-dT) as well as homopolynucleotides poly(dA).poly(dT) and poly(dG).poly(dC) in aqueous solutions with high-salt concentrations (3 M NaCl and 4-6 M CsF), in water-ethanol (60%) solution and in 0.15 M NaCl at 25 degrees C. The rate constants for adenine (kA) and guanine (kG) of polynucleotides were compared with corresponding constants for E. coli DNA. dGMP nd dAMP at the same conditions. The relation between exchange rates and conformations of polynucleotides permits the study of their conformational peculiarities in solution. Of three alternating polynucleotides examined in 0.15 M NaCl the exchange retardation was observed only for poly(dA-dT).poly(dA-dT) as compared with that in B-DNA, which is in good agreement with the B-alternating "wrinkled" DNA model. The conformations of poly(dG-dC).poly(dG-dC) and poly(dA-dC).poly(dG-dT), according to the exchange data obtained are within the B form. For homopolynucleotides in 0.15 M NaCl, the KA value for poly(dA).poly(dT) is nearly the same as kA for B-DNA, which indicates the similarity of their conformations, whereas the kG value for poly(dG).poly(dC) is 1.7-fold lower in comparison with the kG value in B-DNA. This seems to be connected with the existence of B = A conformation equilibrium for poly(dG).poly(dC) in solution. The increase of NaCl concentration to 3 M results in a B----Z transition in the case of poly(dG-dC).poly(dG-dC) and in the shift of B = A equilibrium towards the A-form in the case of poly(dG).poly(dC) as is evidenced by alterations of their KG values. Poly(dA-dT).poly(dA-dT) in 6 M CsF and poly(dA-dC).poly(dG-dT) in 4.3 M CsF maintain their inherent conformations in 0.15 M NaCl in spite of the fact that they are characterised by the "X-type" CD-spectrum at these conditions. According to the exchange data the conformation of poly(dA).poly(dT) in 6 M CsF corresponds to the "heteronomous" DNA model or some other structure with lower accessibility of C8H groups of adenylic residues. 相似文献
4.
Iu B Lishmanov Zh V Trifonova A N Tsibin L V Maslova L A Dement'eva 《Biulleten' eksperimental'no? biologii i meditsiny》1987,103(4):422-424
The experiments on white rats have shown that the induction of 4 hour stress produces an acute increase in beta-endorphin level, as well as characteristic changes in ACTH, cortisol, insulin, thyroxin and triiodothyronine concentrations. Different types of adaptation (training with short stress periods or injection of rhodiola rosea extract) promote a moderate increase in the amount of serum immunoreactive beta-endorphin, preventing its subsequent stress-induced elevation. Adaptation is characterized by a decrease or total prevention of hormonal changes peculiar to stress. The role of opioid neuropeptides in enhancing stress tolerance and the effect of adaptation factors are discussed. 相似文献
5.
G B Maslova D F Avgustinovich 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》1989,39(4):627-632
Participation was studied of central serotonin receptors of the first and second types in behaviour change of animals selected by the character of defensive reaction to man. Serotonin receptors were determined by radioligand method by binding of the brain preparations 3H-serotonin and 3H-spiperone. An increase of C2 receptors number was found in the frontal brain cortex of the tame brown rats in comparison with the aggressive ones. Differences were not found in specific C1-receptor binding in the frontal brain cortex of tame and aggressive brown rats, silver foxes and American minks in various relatively early selection stages. It is supposed that disappearance of aggressive reaction to man at domestication is connected with an increase of C2 receptors number. 相似文献
6.
O K Kutitova G Iu Lipskaia S V Maslova V I Agol 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1989,(11):14-20
Oligonucleotide maps of some poliovirus type 2 strains isolated from polio cases, while being clearly related to that of the Sabin vaccine type 2 strain, exhibited, nevertheless, marked differences from the reference (vaccine) map. Several large oligonucleotides derived from 4 such strains were subjected to enzymatic sequencing. The results strongly suggest that all of them were intertypic recombinants between the Sabin strains. The 5'-parts of the genomes of these strains were derived from the type 2 vaccine whereas the 3'-parts were of type 1 (in 2 strains) or type 3 (in other 2 strains) origin. 相似文献
7.
E A Tolosa R N Maslova E V Goryachenkova I H Willhardt A E Braunstein 《European journal of biochemistry》1975,53(2):429-436
Serine sulphhydrase from chicken liver and cysteine lyase from chicken-embryo yolk sac catalyse the exchange of alpha-H atoms of the amino acid substrate with 3-H-2O. The degree of labelling of the unreacted substrate approaches a maximum of one atom per mol of amino acid. In the absence of replacing agent there is practically no H-exchange in the substrate. The alpha-H of the accumulating beta-substitution product is completely replaced by the labelled hydrogen of the solvent water, irrespective of the duration of incubation. The amount of labelled alpha-hydrogen incorporated into excess (unreacted) amino acids substrate within 3.5-h incubation is somewhat less than the amount incorporated into the product of the complete enzymic beta-replacement reaction. Within the sensitivity limits of detection, the enzymes do not induce any isotopic exchange either of b-H atoms in the amino substrate or of 18-O-labelled beta-HO groups, in the case of L-serine. Neither serine sulphhydrase nor cysteine lyase will catalyse alpha-hydrogen exchange in close structural analogues of their substrates, e.g. L-alanine, D-serine, threonin, 3-phosphoserine. A special case is the interaction of cysteine lyase with the competitive inhibitor, L-serine (whose inhibitor constant, K-i, is equal to the Michaelis constant, K-m, of L-cysteine): the lyase catalyses, only in presence of a cosubstrate thiol, alpha-H exchange in L-serine at approximately the same rate as in L-cysteine. The present data concerning isotopic alpha-H exchange in substrate amino acids, and evidence published earlier, suggest that the catalytic mechanism of replacement-specific beta-lyases may significantly differ from that of the eliminating or ambivalent (mixed-function) lyases. Formation of alpha, beta-unsaturated pyridoxylidene aldimines as real reaction intermediates is unlikely in the case of lyases specifically catalysing beta-replacement reactions; these may proceed by some alternative mechanism of the type suggested in this paper. 相似文献
8.
S. P. Maslova G. N. Tabalenkova R. V. Malyshev T. K. Golovko 《Russian Journal of Plant Physiology》2013,60(6):821-829
Growth and metabolic activity of underground shoots of a long-rhizome perennial herbaceous species yarrow (Achillea millefolium L.) were studied. The active growth of rhizomes and the formation of new meristematic zones were observed during the second half of the growing season after termination of aboveground shoot growth. In this period, underground shoots had a rather high rate of respiration (1.3 mg CO2/(g dry wt h)), a considerable content of nonstructural carbohydrates (15% of dry weight), and the elevated activities of IAA, cytokinins, and ABA. In autumn, the rate of respiration of underground shoots decreased to 1.0 mg CO2/(g dry wt h), soluble sugars accumulated, the ratio between unsaturated and saturated fatty acids rose as well as the ratios GA/ABA and cytokinins/ABA. Temperature optimum for the rhizome growth lay in the range of low and moderate above-zero temperatures (5–20°C), and the freezing point of water in the apices of under-ground shoots was about ?10°C. It is concluded that rhizome quiescence predominantly depends on low temperatures and is not associated with the accumulation of growth inhibitors. In the course of plant preparation to winter, morphogenetic transformations in underground shoots depend on changes in the hormonal balance directed in favor of growth hormones and relatively high respiratory activity in the apical zones of the rhizomes. 相似文献
9.
10.
Kudinova A. G. Petrova M. A. Dolgikh A. V. Soina V. S. Lysak L. V. Maslova O. A. 《Microbiology》2020,89(5):574-584
Microbiology - Increasing interest in microbial communities of Arctic and Antarctic extreme environments, their taxonomic diversity, and forms and mechanisms of adaptation of inhabitants of... 相似文献