全文获取类型
收费全文 | 3626篇 |
免费 | 276篇 |
国内免费 | 3篇 |
出版年
2022年 | 17篇 |
2021年 | 43篇 |
2020年 | 28篇 |
2019年 | 33篇 |
2018年 | 60篇 |
2017年 | 41篇 |
2016年 | 73篇 |
2015年 | 128篇 |
2014年 | 130篇 |
2013年 | 204篇 |
2012年 | 224篇 |
2011年 | 233篇 |
2010年 | 134篇 |
2009年 | 133篇 |
2008年 | 219篇 |
2007年 | 227篇 |
2006年 | 199篇 |
2005年 | 224篇 |
2004年 | 206篇 |
2003年 | 158篇 |
2002年 | 192篇 |
2001年 | 75篇 |
2000年 | 59篇 |
1999年 | 74篇 |
1998年 | 60篇 |
1997年 | 43篇 |
1996年 | 39篇 |
1995年 | 33篇 |
1994年 | 32篇 |
1993年 | 18篇 |
1992年 | 45篇 |
1991年 | 49篇 |
1990年 | 34篇 |
1989年 | 53篇 |
1988年 | 41篇 |
1987年 | 29篇 |
1986年 | 33篇 |
1985年 | 30篇 |
1984年 | 28篇 |
1983年 | 21篇 |
1982年 | 23篇 |
1981年 | 12篇 |
1980年 | 12篇 |
1979年 | 12篇 |
1978年 | 13篇 |
1977年 | 12篇 |
1976年 | 15篇 |
1975年 | 12篇 |
1974年 | 14篇 |
1968年 | 9篇 |
排序方式: 共有3905条查询结果,搜索用时 281 毫秒
1.
Carlo L. Seifert Martin Volf Leonardo R. Jorge Tomokazu Abe Grace Carscallen Pavel Drozd Rajesh Kumar Greg P.A. Lamarre Martin Libra Maria E. Losada Scott E. Miller Masashi Murakami Geoffrey Nichols Petr Pyszko Martin igut David L. Wagner Vojtch Novotný 《Ecology and evolution》2020,10(24):14137
- Assemblages of insect herbivores are structured by plant traits such as nutrient content, secondary metabolites, physical traits, and phenology. Many of these traits are phylogenetically conserved, implying a decrease in trait similarity with increasing phylogenetic distance of the host plant taxa. Thus, a metric of phylogenetic distances and relationships can be considered a proxy for phylogenetically conserved plant traits and used to predict variation in herbivorous insect assemblages among co‐occurring plant species.
- Using a Holarctic dataset of exposed‐feeding and shelter‐building caterpillars, we aimed at showing how phylogenetic relationships among host plants explain compositional changes and characteristics of herbivore assemblages.
- Our plant–caterpillar network data derived from plot‐based samplings at three different continents included >28,000 individual caterpillar–plant interactions. We tested whether increasing phylogenetic distance of the host plants leads to a decrease in caterpillar assemblage overlap. We further investigated to what degree phylogenetic isolation of a host tree species within the local community explains abundance, density, richness, and mean specialization of its associated caterpillar assemblage.
- The overlap of caterpillar assemblages decreased with increasing phylogenetic distance among the host tree species. Phylogenetic isolation of a host plant within the local plant community was correlated with lower richness and mean specialization of the associated caterpillar assemblages. Phylogenetic isolation had no effect on caterpillar abundance or density. The effects of plant phylogeny were consistent across exposed‐feeding and shelter‐building caterpillars.
- Our study reveals that distance metrics obtained from host plant phylogeny are useful predictors to explain compositional turnover among hosts and host‐specific variations in richness and mean specialization of associated insect herbivore assemblages in temperate broadleaf forests. As phylogenetic information of plant communities is becoming increasingly available, further large‐scale studies are needed to investigate to what degree plant phylogeny structures herbivore assemblages in other biomes and ecosystems.
2.
With special interest in the mode of action of zwitterionic detergents on proteins, a variety of detergents were examined for their ability to disrupt the secondary and quaternary structures of an anion transport protein, band 3, and its cytoplasmic 38 kDa fragment from bovine erythrocyte membranes and for their effect on the binding of an anion transport inhibitor to band 3. Nonionic detergents and Chaps also acted as a nondenaturant in these instances, as well accepted for other proteins. Though deoxycholate and cholate inhibited the binding of an anion transport inhibitor to band 3, these detergents did not show any effect on the native structure of band 3. Zwitterionic detergents (Zwittergent 3-10, Zwittergent 3-12 and N, N-dimethyl-N-dodecyl glycine) were suggested to denature the water-soluble 38 kDa fragment at concentrations above the critical micelle concentration, but to be weak in disrupting interacting forces between hydrophobic membrane-bound domains of band 3. The results indicated that these zwitterionic detergents are similar in the mode of denaturing action to dodecyltrimethylammonium bromide rather than sodium dodecyl sulfate. 相似文献
3.
Kenji Tsuji Shinji KitamuraHirofumi Makino 《Biochemical and biophysical research communications》2014
The kidneys are exposed to hypoxic conditions during development. Hypoxia-inducible factor (HIF), an important mediator of the response to hypoxia, is believed to have an important role in development. However, the relationship between HIF and branching morphogenesis has not been elucidated clearly. 相似文献
4.
Koichi Inoue Akiko Yamaguchi Megumi Wada Yoshihiro Yoshimura Tsunehisa Makino Hiroyuki Nakazawa 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2001,765(2):3469-126
Due to the ubiquity of epoxy resin compounds and their potential role in increasing the risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed a method for measuring bisphenol A (BPA) and bisphenol A diglycidyl ether (BADGE) metabolites in human blood samples using high-performance liquid chromatography–electrospray ionization mass spectrometry (LC–MS). Human blood samples were processed using enzymatic deconjugation of the glucuronides followed by a novel sample preparation procedure using a solid-phase-cartridge column. This selective analytical method permits rapid detection of the metabolites, free BPA and a hydrolysis product of BADGE (BADGE-4OH) with detection limits in the low nanogram per milliliter range (0.1 ng ml−1 of BPA and 0.5 ng ml−1 of BADGE-4OH). The sample extraction was achieved by Oasis HLB column on gradient elution. The recoveries of BPA and BADGE-4OH added to human plasma samples were above 70.0% with a standard deviation of less than 5.0%. This selective, sensitive and accurate method will assist in elucidating potential associations between human exposure to epoxy-based compounds and adverse health effects. 相似文献
5.
6.
Sajiro Makino 《Chromosoma》1950,3(1):220-231
Summary The chromosome complex of Dall's porpoise Phocoenoides dallii (True), a species of the Delphinidae (Cetacea), was investigated in male germ cells during the course of spermatogenesis. The diploid number of chromosomes in this species was 44 in the spermatogonia and the haploid number was 22 in both primary and secondary spermatocytes. Sex chromosomes of the typical XY-type were found to occur in this species. The X element is represented by one of the medium-sized chromosomes of rod-type characterized by a globular body located at its inner extremity, while the Y is very minute, attaining a size approximately one third that of the smallest autosome.Morphological analysis of the chromosomes shows the chromosome complement of this species to be strikingly characterized by the prevalence of medium-sized elements having subterminal fibre attachments. Comparison of the chromosomes with those of related forms of mammals shows that the chromosome constitution of this species approximates closely that of the pig. The question of the phylogenetical affinity of the Cetacea was discussed on the basis of the karyological evidence here reported.Contribution No. 213 form the Zoological Institute, Faculty of Science, Hokkaido University, Sapporo, Japan. 相似文献
7.
8.
9.
K Sakurai N Seki R Fujii K Yagui Y Tokuyama F Shimada H Makino Y Suzuki N Hashimoto Y Saito T Egashira K Matsui A Kanatsuka 《Hormones et métabolisme》2000,32(8):316-320
Mutations of the hepatocyte nuclear factor 4 alpha (HNF-4alpha) gene have been demonstrated in maturity-onset diabetes of the young (MODY) 1 families. To investigate the possibility that the HNF-4alpha gene contributes to the onset of non-insulin-dependent diabetes mellitus (NIDDM) in Japanese patients, we screened all exons and flanking introns of this gene for mutations in 100 patients with NIDDM diagnosed after 25 years of age. We identified two missense mutations: M49V in exon 1c and T1301 in exon 4; and two nucleotide substitutions in introns: cytosine to thymidine at -5 nt in intron 1b and adenine to thymidine at -21 nt in intron 5. We screened an additional 220 diabetic subjects for the polymorphism in intron 1b. The c/t substitution in intron 1b was associated with NIDDM. This substitution in the polypyrimidine tract, an important cis-acting element directing intron removal, is likely to influence pre-mRNA splicing of this gene. T1301 in exon 4 was observed in only two diabetic subjects. This mutation could influence the conformation of this peptide, resulting in changes in ligand binding domain function. M49V in exon 1c was found in both diabetic and non-diabetic subjects; isoforms HNF-4alpha 4, 5, and 6 with this mutation may impair glucose metabolism in tissue. In contrast to the primary cause of nonsense and missense mutations of the HNF-4alpha gene in MODY1, the nucleotide substitution in intron 1b may partially contribute to development of NIDDM in combination with other genetic and environmental factors. 相似文献
10.
Inhibition of Na+-Ca2+ exchange in heart sarcolemmal vesicles by phosphatidylethanolamine N-methylation 总被引:1,自引:0,他引:1
The effect of phosphatidylethanolamine N-methylation on Na+-Ca2+ exchange was studied in sarcolemmal vesicles isolated from rat heart. Phosphatidylethanolamine N-methylation following incubation of membranes with S-adenosyl-L-methionine, a methyl donor for the enzymatic N-methylation, inhibited Nai+-dependent Ca2+ uptake by about 50%. The N-methylation reaction did not alter the passive permeability of the sarcolemmal vesicles to Na+ and Ca2+ and did not modify the electrogenic characteristics of the exchanger. The depressant effect of phosphatidylethanolamine N-methylation on Nai+-dependent Ca2+ uptake was prevented by S-adenosyl-L-homocysteine, an inhibitor of the N-methylation. Pretreatment of sarcolemma with methyl acetimidate hydrochloride, an amino-group-blocking agent, also prevented methylation-induced inhibition of Ca2+ uptake. In the presence of exogenous phospholipid substrate, the phospholipid N-methylation process in methyl-acetimidate-treated sarcolemmal vesicles was restored and the inhibitory effect on Ca2+ uptake was evident. These results suggest that phosphatidylethanolamine N-methylation influences the heart sarcolemmal Na+-Ca2+ exchange system. 相似文献