全文获取类型
收费全文 | 3858篇 |
免费 | 265篇 |
国内免费 | 1篇 |
专业分类
4124篇 |
出版年
2022年 | 24篇 |
2021年 | 45篇 |
2020年 | 30篇 |
2019年 | 39篇 |
2018年 | 47篇 |
2017年 | 40篇 |
2016年 | 71篇 |
2015年 | 109篇 |
2014年 | 140篇 |
2013年 | 169篇 |
2012年 | 186篇 |
2011年 | 252篇 |
2010年 | 167篇 |
2009年 | 141篇 |
2008年 | 230篇 |
2007年 | 229篇 |
2006年 | 209篇 |
2005年 | 206篇 |
2004年 | 188篇 |
2003年 | 194篇 |
2002年 | 182篇 |
2001年 | 128篇 |
2000年 | 116篇 |
1999年 | 82篇 |
1998年 | 39篇 |
1997年 | 35篇 |
1996年 | 29篇 |
1995年 | 45篇 |
1994年 | 31篇 |
1993年 | 31篇 |
1992年 | 53篇 |
1991年 | 58篇 |
1990年 | 49篇 |
1989年 | 63篇 |
1988年 | 47篇 |
1987年 | 40篇 |
1986年 | 53篇 |
1985年 | 42篇 |
1984年 | 23篇 |
1983年 | 20篇 |
1982年 | 20篇 |
1981年 | 15篇 |
1980年 | 23篇 |
1979年 | 24篇 |
1978年 | 18篇 |
1977年 | 24篇 |
1976年 | 14篇 |
1974年 | 19篇 |
1973年 | 14篇 |
1971年 | 14篇 |
排序方式: 共有4124条查询结果,搜索用时 15 毫秒
1.
The absorption spectrum of light-adapted purple membrane in 3 M KCl is dependent on temperature even in the room temperature region. Temperature-induced difference spectra at various pH values suggested that the trans isomer of bacteriorhodopsin, bR570, is in thermal and/or photodynamic equilibrium with several different conformers. The major second conformer occurring at neutral pH had the same spectroscopic properties as the 13-cis isomer, and its content at 35 degrees C was estimated to be more than 20%. Heterogeneity in the protein conformation became more significant above pH8, where temperature-induced difference spectra exhibited a negative peak at 580 nm and a positive peak at 296 nm. This absorption change is very similar to that observed upon the formation of the N intermediate, suggesting that an N-like conformer occurs at high pH and temperature. A significant temperature dependence was also seen in the M decay kinetics at high pH, which were described by two decay components; i.e., the fast decaying M (Mf) was predominant at low temperature, but the amplitude of the slow component (M(s)) increased with increasing temperature. It is suggested that M(s) is generated upon excitation of the N-like conformer, in which the residue (Asp-96) usually acting as a proton donor to the Schiff base is deprotonated. The N-like conformer could be N itself, because M(s) was enhanced when N was accumulated by background light. A strong correlation between the amplitude of M(s) and the concentration of N was also revealed by the accumulation kinetics of Mf, M(s), and N after the onset of continuous actinic light.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
2.
Shigeo Koyasu Makoto Asada Akio Fukuda Yoshimi Okada 《Journal of molecular biology》1981,153(2):471-475
The mode of polymerization of two species of flagellins, flagellin A and flagellin B, in polar flagella of Caulobacter crescentus was examined. By immunological staining we found that 1 to 1.2 μm of the portion of the flagellar filament proximal to the cell was composed of flagellin B, whereas about 5 μm of the distal portion was composed of flagellin A. This result, together with the previous observation that a flagellin B-less mutant cannot form normal flagella but instead forms stubs in spite of their high level of flagellin A synthesis, indicates that flagellin B is very important for the formation of complete flagella and/or for the initiation of filament formation from the hook. 相似文献
3.
Yasuji Fukuda 《Journal of plant research》1982,95(2):183-194
Seedling morphology and vascular course inTribulus terrestris were studied. This species has no erect stem, but four buds appear immediately above the cotyledonary node and grow into prostrate shoots. They were determined to be the main axis of the seedling and the axillary branches of the earliest three foliage leaves, which arise very close to each other. All the leaves, including cotyledons, are vascularized with four bundles among which two are related to a single median gap. When two leaves are attached to one node, lateral traces to the opposed leaves are derived by bifurcation of a single bundle at either side of the stem. In the shoot with a series of alternate leaves, the median pair of traces to every other leaf are found on the same orthostichy. In the branch of which the first node bears no flower but an anisophyllous pair of leaves, the smaller leaf at the node was proven to be the first prophyll because its median traces are superposed by those to the leaf at the next node. 相似文献
4.
5.
6.
7.
8.
9.
Summary The wall of the cerebral aqueduct was examined in 20 male rats at the light- and electron-microscopic levels. Disorder in ciliary orientation was occasionally seen in ordinary ependymal cells. Ependymal cells possessing intracellular cysts of 5 to 30 urn in diameter were observed within and beneath the aqueductal ependyma in all animals examined. Light-microscopic reconstruction from serial, 10-m thick frontal sections revealed an extensive distribution of cystic ependymal cells (CECs), especially along the ependymal ridges in the rostral half of the aqueduct, and along the dorsal region of the aqueductal lining in the caudal half. Both cystic and surface membranes of CECs bore microvilli and cilia. Ectopic ependymal cells (EECs) characterized by densely packed microvilli, well-developed intermediate junctions and cilia, but without cysts, were situated in the subependymal region adjacent to a CEC or another EEC. The ependymal ridges were long, narrow and sporadically stratified ependymal linings extending rostrocaudally and bilaterally along the aqueductal surface. Tanycyte-like cells filled the surface region of the ridge, and CECs and EECs were frequently seen in the core. Intraventricularly injected microperoxidase was detected among densely packed microvilli but not in the cystic lumina of CECs, indicating that EECs and CECs are distinct entities. 相似文献
10.
Takashi Ooba Hideyuki Hayashi Sachiko Karaki Manabu Tanabe Kyoichi Kano Masafumi Takiguchi 《Immunogenetics》1989,30(2):76-80
The primary structure ofHLA-B51 andHLA-Bw52 suggested thatHLA-B51 was derived fromHLA-Bw52 by the combination of a genetic exchange withHLA-B8 and a point mutation. To investigate the evolution of theHLA-B5 cross reactive group, theHLA-B35 gene was cloned and the primary structure was determined.HLA-B35 is identical toHLA-Bw58 except in the α1 domain. The α1 domain ofHLA-B35 except Bw4/Bw6-associated amino acids is identical to that ofHLA-B51
*, which was suspected to be an intermediate gene betweenHLA-B51 andHLA-Bw52. These data suggest thatHLA-B35 has evolved fromHLA-Bw58 in two steps; an in vivo replacement of the α1 domain withHLA-B51 and genetic exchange with one of theHLA-Bw6 genes. These three genes andHLA-Bw58 are postulated to share a common ancestor. 相似文献