全文获取类型
收费全文 | 1904篇 |
免费 | 152篇 |
出版年
2023年 | 8篇 |
2022年 | 21篇 |
2021年 | 40篇 |
2020年 | 26篇 |
2019年 | 30篇 |
2018年 | 39篇 |
2017年 | 42篇 |
2016年 | 53篇 |
2015年 | 93篇 |
2014年 | 113篇 |
2013年 | 116篇 |
2012年 | 165篇 |
2011年 | 168篇 |
2010年 | 91篇 |
2009年 | 86篇 |
2008年 | 116篇 |
2007年 | 115篇 |
2006年 | 87篇 |
2005年 | 93篇 |
2004年 | 78篇 |
2003年 | 66篇 |
2002年 | 89篇 |
2001年 | 22篇 |
2000年 | 20篇 |
1999年 | 29篇 |
1998年 | 18篇 |
1997年 | 11篇 |
1996年 | 20篇 |
1995年 | 14篇 |
1994年 | 15篇 |
1993年 | 12篇 |
1992年 | 22篇 |
1991年 | 12篇 |
1990年 | 12篇 |
1989年 | 11篇 |
1988年 | 5篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 9篇 |
1982年 | 5篇 |
1979年 | 5篇 |
1977年 | 5篇 |
1976年 | 4篇 |
1975年 | 4篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 5篇 |
1970年 | 4篇 |
1969年 | 4篇 |
排序方式: 共有2056条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
The morphological changes in the gill chloride cells of the armored catfish, Hypostomus tietensis , were investigated after 15 days' exposure to either distilled or hard water. The thickness of the water–blood barrier in the lamellae increased significantly in fish kept in distilled water due to the high proliferation of chloride cells. The apical surface of about 68% of chloride cells was sharply reduced by the development of an apical crypt with a sponge-like surface, although no change in the chloride cell fractional area was found. In contrast, H. tietensis kept in Na+ , Cl− and Ca2+ rich water displayed no significant changes in the number of chloride cells or in their apical surface morphology compared with the control fish. Chloride cell response to ion challenge in H. tietensis suggested the involvement of different strategies to maintain homeostasis in ion-poor water, which may be related to the life history of species. 相似文献
5.
Rapid immunofluorescent determination of cells in the S phase in pea root meristems: An alternative to autoradiography 总被引:2,自引:0,他引:2
Photosystem II (PS II) activity and the localization of ribulose-l,5-bisphosphate (RuBP) carboxylase (EC 4.1.1.39) were studied in primary leaves of young maize plants ( Zea mays L. cv. Fronica) by tetra-nitro-blue-tetrazoliumchloride reduction and immunolocalization, respectively. In tissue of 3-day-old plants all chloroplasts were structurally identical. From day 4 they developed into their typical appearance of mesophyll and bundle sheath chloroplasts. First PS II-activity was present in both types of chloroplasts. From day 4 it disappeared in bundle sheath chloroplasts concomitant with the loss of grana. RuBP carboxylase on the other hand was only present in bundle sheath chloroplasts at all stages of development. Thus, the control of the development of the photosystems and the Calvin cycle enzymes seem to differ. 相似文献
6.
Summary Genetic determinants of the Muc+ character were investigated in two ropy strains,Lactobacillus delbrueckii ssp.bulgaricus 201 andL. casei ssp.casei NCIB 4114, which secrete a large amount of slime in culture media. Plasmid DNA analysis revealed the presence of two plasmids (4.5 and 2.3 Mdal) inL. casei ssp.casei, whileL. delbrueckii ssp.bulgaricus was plasmid free, suggesting a chromosomal location of Muc+ character in this strain. Curing experiments carried out onL. casei ssp.casei NCIB 4114 indicated a correlation between the Muc+ phenotype and the 4.5 Mdal plasmid. 相似文献
7.
Piero Pollesello Renato Toffanin Erminio Murano Roberto Rizzo Sergio Paoletti Bjarne J. Kvam 《Journal of applied phycology》1992,4(2):149-155
Lipid extracts of the red algaGracilaria longa were studied by1H- and13C-NMR spectroscopy. Peaks in the13C-NMR spectra attributable to sterols, chlorophylls and carotenoids allowed free and acylated cholesterol, chlorophylla and lutein to be identified as the most abundant components of these classes. A content of 0.5 ± 0.1 μmoles of total cholesterol/g
wet alga was estimated from the1H-NMR spectrum, which also allowed the determination of the phosphatidylcholine/total lipid molar ratio (9.5 ± 0.5%). The13C-NMR spectroscopic experiments provided information on the position of the double bonds on the fatty acid residues. A comparison
between NMR spectra of lipid extracts obtained for wet and dried alga showed that the alga undergoes both a dramatic peroxidation
and some glycolipid degradation during the drying process. 相似文献
8.
9.
Sergio Sgorbati Marisa Levi Elio Sparvoli Federica Trezzi Giovanni Lucchini 《Physiologia plantarum》1986,68(3):471-476
Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA-DAPI complex in nuclei released from different fresh and formaldehyde-fixed pea ( Pisum sativum L. cv. Lincoln) tissues. The two methods of isolation are compared and discussed as well as their possible use for quantitative analysis of DNA in plant tissues. With fixed tissues it is possible to obtain a number of nuclei sufficient for the flow cytometric analysis, even using small amounts of plant tissue. 相似文献
10.