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C Crone J Frokjaer-Jensen JJ Friedman O Christensen 《The Journal of general physiology》1978,71(2):195-220
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Synchronous growth of a freshwater diatom Melosira italica under natural environment 总被引:1,自引:0,他引:1
Summary A filamentous diatom Melosira italica was collected at the beginning of rainy season from a shallow lake in the tropical savanna region in Brazil. Even the sample taken from surface water contained empty cells in high percentages. The number of cells per filament of M. italica showed a peculiar pulse-like frequency distribution with peak values at 4, 8, 12 and 16. Evidences of the synchronous cell division in this planktonic diatom under natural environment are discussed. 相似文献
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Sascha Naomi McKeon Marta Moreno Maria Anise Sallum Marinete Marins Povoa Jan Evelyn Conn 《Memórias do Instituto Oswaldo Cruz》2013,108(5):605-615
To evaluate whether environmental heterogeneity contributes to the
genetic heterogeneity in Anopheles triannulatus, larval habitat
characteristics across the Brazilian states of Roraima and Pará and genetic
sequences were examined. A comparison with Anopheles goeldii
was utilised to determine whether high genetic diversity was unique to
An. triannulatus. Student t test and
analysis of variance found no differences in habitat characteristics between the
species. Analysis of population structure of An. triannulatus
and An. goeldii revealed distinct demographic histories in a
largely overlapping geographic range. Cytochrome oxidase I
sequence parsimony networks found geographic clustering for both species;
however nuclear marker networks depicted An. triannulatus with
a more complex history of fragmentation, secondary contact and recent
divergence. Evidence of Pleistocene expansions suggests both species are more
likely to be genetically structured by geographic and ecological barriers than
demography. We hypothesise that niche partitioning is a driving force for
diversity, particularly in An. triannulatus. 相似文献
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Daniela Volcan Almeida Camila de Martinez Gaspar Martins M��rcio de Azevedo Figueiredo Carlos Frederico Ceccon Lanes Adalto Bianchini Luis Fernando Marins 《Transgenic research》2013,22(1):75-88
Growth hormone (GH) transgenic fish are at a critical step for possible approval for commercialization. Since this hormone is related to salinity tolerance in fish, our main goal was to verify whether the osmoregulatory capacity of the stenohaline zebrafish (Danio rerio) would be modified by GH-transgenesis. For this, we transferred GH-transgenic zebrafish (T) from freshwater to 11 ppt salinity and analyzed survival as well as relative changes in gene expression. Results show an increased mortality in T versus non-transgenic (NT) fish, suggesting an impaired mechanism of osmotic acclimation in T. The salinity effect on expression of genes related to osmoregulation, the somatotropic axis and energy metabolism was evaluated in gills and liver of T and NT. Genes coding for Na+, K+-ATPase, H+-ATPase, plasma carbonic anhydrase and cytosolic carbonic anhydrase were up-regulated in gills of transgenics in freshwater. The growth hormone receptor gene was down-regulated in gills and liver of both NT and T exposed to 11 ppt salinity, while insulin-like growth factor-1 was down-regulated in liver of NT and in gills of T exposed to 11 ppt salinity. In transgenics, all osmoregulation-related genes and the citrate synthase gene were down-regulated in gills of fish exposed to 11 ppt salinity, while lactate dehydrogenase expression was up-regulated in liver. Na+, K+-ATPase activity was higher in gills of T exposed to 11 ppt salinity as well as the whole body content of Na+. Increased ATP content was observed in gills of both NT and T exposed to 11 ppt salinity, being statistically higher in T than NT. Taking altogether, these findings support the hypothesis that GH-transgenesis increases Na+ import capacity and energetic demand, promoting an unfavorable osmotic and energetic physiological status and making this transgenic fish intolerant of hyperosmotic environments. 相似文献
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Sean M. Griffing Giselle M. Rachid Viana Tonya Mixson-Hayden Sankar Sridaran Mohammad Tauqeer Alam Alexandre Macedo de Oliveira John W. Barnwell Ananias A. Escalante Marinete Marins Povoa Venkatachalam Udhayakumar 《PloS one》2013,8(3)
Previous work suggests that Brazilian Plasmodium falciparum has limited genetic diversity and a history of bottlenecks, multiple reintroductions due to human migration, and clonal expansions. We hypothesized that Brazilian P. falciparum would exhibit clonal structure. We examined isolates collected across two decades from Amapá, Rondônia, and Pará state (n = 190). By examining more microsatellites markers on more chromosomes than previous studies, we hoped to define the extent of low diversity, linkage disequilibrium, bottlenecks, population structure, and parasite migration within Brazil. We used retrospective genotyping of samples from the 1980s and 1990s to explore the population genetics of SP resistant dhfr and dhps alleles. We tested an existing hypothesis that the triple mutant dhfr mutations 50R/51I/108N and 51I/108N/164L developed in southern Amazon from a single origin of common or similar parasites. We found that Brazilian P. falciparum had limited genetic diversity and isolation by distance was rejected, which suggests it underwent bottlenecks followed by migration between sites. Unlike Peru, there appeared to be gene flow across the Brazilian Amazon basin. We were unable to divide parasite populations by clonal lineages and pairwise FST were common. Most parasite diversity was found within sites in the Brazilian Amazon, according to AMOVA. Our results challenge the hypothesis that triple mutant alleles arose from a single lineage in the Southern Amazon. SP resistance, at both the double and triple mutant stages, developed twice and potentially in different regions of the Brazilian Amazon. We would have required samples from before the 1980s to describe how SP resistance spread across the basin or describe the complex internal migration of Brazilian parasites after the colonization efforts of past decades. The Brazilian Amazon basin may have sufficient internal migration for drug resistance reported in any particular region to rapidly spread to other parts of basin under similar drug pressure. 相似文献