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1.
Seven different hosts,Panicum maximum, Chrysopogon fulvis, Themeda triandra, Chloris gayana, Brachiaria brizantha, Paspalum scrobiculatum andEleusine coracana were screened in order to select a better host for mass multiplication ofGlomus fasciculatum inoculum. Of these,Chloris gayana (Rhodes grass) was found to be the best host on the basis of root colonization and spore production and of the infective
propagules of the potball. 相似文献
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V. Sreenivasa Reddy Kattesh V. Katti Charles L. Barnes 《Inorganica chimica acta》1995,240(1-2):367-370
The water-soluble bisphosphine, 1,2-bis(bis(hydroxymethyl)phosphino)ethane (1), was synthesized in near quantitative yield by the reaction of bisphosphine, H2PCH2CH2PH2, with an aqueous formaldehyde in the presence of K2PtCl4. The reaction of this water-soluble bisphosphine 1 with cis-Pt(COD)Cl2 affords the mononuclear bischelate complex, [Pt{(HOH2C)2PCH2CH2P(CH2OH)2}2](Cl)2 (2), in near quantitative yield. The new ligand and complex have been characterized spectroscopically and the structure of the metal complex, 2, was determined by X-ray crystallography. The Pt(II) complex 2 crystallizes in the orthorhombic space group Pbca(a=14.623(1), B=16.216(2), C=9.319(4) Å) with Z=4. The final R value is 0.024. 相似文献
3.
K Sreenivasa Moorthy B Kasi Reddy K S Swami C Sreeramulu Chetty 《Archives internationales de physiologie et de biochimie》1984,92(3):147-151
Effects in vitro of methyl parathion on some kinetic constants of succinic dehydrogenase (SDH) in hepatopancreas of freshwater mussel, L. marginalis were studied. Altered pH vs. specific activity curves for SDH demonstrated significant inhibition by methyl parathion in buffered acidic, neutral and alkaline ranges. At high pH ranges IC50 (12.5 microM) of methyl parathion did not cause 50% inhibition enzyme as it did at neutral and acidic pHs. Activation energies (delta E) were found to be increased suggesting decreased efficiency of enzyme in presence of methyl parathion. Non-competitive inhibition with respect to activation by succinate was indicated by decreased maximal velocity (V) without change in Michaelis Menten constant (Km). Pyridine-2-aldoxime (25 microM), pyridine-4-aldoxime (15 microM) and L-cysteine (40 microM) neutralized the inhibition of SDH by methyl parathion (12.5 microM). The kinetic data suggests that inhibition of SDH by methyl parathion was pH and temperature independent. 相似文献
4.
Rao K Poornachandra Kumar N Hemanth Somashekaraiah Rakesh Murali M. J Shobha Sreenivasa M. Y. 《Microbiology》2021,90(3):361-369
Microbiology - Manufacture of fermented foods/products, which, apart from basic nutrition, have health-promoting effects, is flourishing. Within the field of fermented foods, rapidly expanding is... 相似文献
5.
P.N. Achar M.Y. Sreenivasa G. Galdo 《Archives Of Phytopathology And Plant Protection》2013,46(2):220-227
The presence of Aspergillus species is an indicator of storage conditions, which also suggests the possibility of several biochemical changes in grains. A comparative change in total soluble proteins and protease activity was determined in commercial peanut seeds collected from Georgia State. Protein contents of healthy peanuts, naturally contaminated peanuts and then artificially inoculated peanut seeds with A. flavus were estimated by Bradford method, and protease activity was also determined by using the Protease Detection Kits. Protein contents and the protease activity of the peanuts varied from sample to sample. The soluble protein content of seeds was significantly higher in healthy peanuts than in artificially inoculated or naturally infected peanuts with A. flavus. Protease activity was found to be higher in artificially inoculated seeds than in either naturally infected or healthy peanuts. Level of soluble proteins in buffer extracts of contaminated seeds decreased with incubation time, and protease activity increased with incubation time. These changes may be attributed to host response due to infection, contribution by A. flavus or due to biochemical alterations that occur naturally during the transition from endosperm to seedling during incubation period. 相似文献
6.
Amal A. Alotaibi Asmatanzeem Bepari Rasha Assad Assiri Shaik Kalimulla Niazi Sreenivasa Nayaka Muthuraj Rudrappa Shashiraj Kareyellapa Nagaraja Meghashyama Prabhakara Bhat 《Current issues in molecular biology》2021,43(2):1114
Background and Objectives: Saussurea lappa (S. lappa) is an important species of the Asteraceae family with several purposes in traditional medicine. This study intended to explore the cytotoxic effect of S. lappa on HepG2 cancer cell proliferation. Materials and Methods: The effects of an S. lappa n-butanol extract on the induction of apoptosis were investigated by flow cytometry and mitochondrial cytochrome C-releasing apoptosis assay. Additionally, real-time PCR was employed to confirm apoptosis initiation. Further, qualitative estimation of the active constituent of S. lappa was done by gas chromatography–mass spectroscopy (GC–MS). Results: The cell viability study revealed that the n-butanol extract of S. lappa demonstrated potent cytotoxicity against HepG2 cancer cells, with an IC50 value of 56.76 μg/mL. Cell morphology with dual staining of acridine orange (AO)-ethidium bromide (EB) showed an increase in orange/red nuclei due to cell death by S. lappa n-butanol extract compared to control cells. Apoptosis, as the mode of cell death, was also confirmed by the higher release of cytochrome C from mitochondria, the increased expression of caspase-3 and bax, along with down regulation of Bcl-2. Conclusion: These findings conclude that S. lappa is a cause of hepatic cancer cell death through apoptosis and a potential natural source suggesting furthermore investigation of its active compounds that are responsible for these observed activities. 相似文献
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Ganji Purna Chandra Nagaraju Nunna Siva Kumari Ganji Lakshmi Vara Prasad Balney Rajitha Madan Meenu Manam Sreenivasa Rao Bannoth Reddya Naik 《Bioinformation》2009,4(1):6-11
The tentative elucidation of the 3D-structure of vitellogenesis inhibiting hormone (VIH)
peptides is conversely underprivileged by difficulties in gaining enough peptide or protein,
diffracting crystals, and numerous extra technical aspects. As a result, no structural
information is available for VIH peptide sequences registered in the Genbank. In this
situation, it is not surprising that predictive methods have achieved great interest. Here, in
this study the molt-inhibiting hormone (MIH) of the kuruma prawn (Marsupenaeus
japonicus) is used, to predict the structure of four VIHrelated peptides in the
crustacean species. The high similarity of the 3D-structures and the calculated physiochemical
characteristics of these peptides suggest a common fold for the entire family. 相似文献
9.
Chun-Ho Wong Yuan Fu Sreenivasa Rao Ramisetty Anne M. Baranger Steven C. Zimmerman 《Nucleic acids research》2011,39(20):8881-8890
Myotonic dystrophy type 2 (DM2) is an incurable neuromuscular disease caused by expanded CCUG repeats that may exhibit toxicity by sequestering the splicing regulator MBNL1. A series of triaminotriazine- and triaminopyrimidine-based small molecules (ligands 1–3) were designed, synthesized and tested as inhibitors of the MBNL1–CCUG interaction. Despite the structural similarities of the triaminotriazine and triaminopyrimidine units, the triaminopyrimidine-based ligands bind with low micromolar affinity to CCUG repeats (Kd ∼ 0.1–3.6 µM) whereas the triaminotriazine ligands do not bind CCUG repeats. Importantly, these simple and small triaminopyrimidine ligands exhibit both strong inhibition (Ki ∼ 2 µM) of the MBNL1–CCUG interaction and high selectivity for CCUG repeats over other RNA targets. These experiments suggest these compounds are potential lead agents for the treatment of DM2. 相似文献
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