排序方式: 共有5条查询结果,搜索用时 15 毫秒
1
1.
Gisele Grimber Colette Galand Michel Garbarz Marie-Genevieve Mattei Catherine Cavard Alain Zider Patrice Blanchet Pierre Boivin Pascale Briand Didier Dhermy 《Transgenic research》1992,1(6):268-274
In the process of generating transgenic mice, inserted foreign DNA can cause insertional inactivation of the flanking genetic
locus and simultaneously provide a molecular tag for localizing and cloning the inactivated gene. We describe the case of
an insertional mutation leading, in animals homozygous for the insertion, to severe anaemia that was lethal within a few days
after birth. The haemolytic anaemia and microspherocytosis of the red cells strongly suggested membrane abnormalities of the
erythrocytes. Byin situ localization of the integration site, protein analysis of the red cell membranes, northern and Southern blot analyses, we
were able to demonstrate that the integrated transgene had affected the α-spectrin gene locus. 相似文献
2.
3.
Cohen Jose L. Boyer Olivier Salamon Benoit Onclercq Rosine Depetris Daniele Lejeune Laurence Dubus-Bonnet Vanessa Bruel Sylvie Charlotte Frederic Mattei Marie-Genevieve Klatzmann David 《Transgenic research》1998,7(5):321-330
Dividing cells expressing the Herpes simplex type 1 thymidine kinase (TK) can be killed upon ganciclovir treatment. Likewise, conditional cell knock-out can be obtained in transgenic mice expressing a TK gene placed under the control of tissue-specific regulatory sequences. Such animals provide powerful experimental systems for assessing the functional role of specific cell populations through their time-controlled ablation. However, whatever the regulatory sequences used, a leaky toxic overexpression of TK in testis renders male TK-transgenic mice sterile and prevents the generation of homozygous TK-expressing animals. To solve this problem, we designed a truncated TK variant (TK) not expressed in the testis. We generated transgenic mice expressing TK under the control of lymphocyte-specific regulatory sequences derived from the CD4 gene. The TK protein expressed in T-lymphocytes allowed the conditional ablation of activated T-cells in vitro and in vivo. Importantly, for one transgenic line we could generate fertile homozygous mice harboring a functional TK transgene. TK should thus dramatically facilitate the development of transgenic mice expressing a conditional suicide gene. 相似文献
4.
Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. 总被引:11,自引:0,他引:11
Jenny Valladeau Valérie Clair-Moninot Colette Dezutter-Dambuyant Jean-Jacques Pin Adrien Kissenpfennig Marie-Genevieve Mattéi Smina Ait-Yahia Elizabeth E M Bates Bernard Malissen Franz Koch Fran?ois Fossiez Nikolaus Romani Serge Lebecque Sem Saeland 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(2):782-792
Human (h)Langerin/CD207 is a C-type lectin of Langerhans cells (LC) that induces the formation of Birbeck granules (BG). In this study, we have cloned a cDNA-encoding mouse (m)Langerin. The predicted protein is 66% homologous to hLangerin with conservation of its particular features. The organization of human and mouse Langerin genes are similar, consisting of six exons, three of which encode the carbohydrate recognition domain. The mLangerin gene maps to chromosome 6D, syntenic to the human gene on chromosome 2p13. mLangerin protein, detected by a mAb as a 48-kDa species, is abundant in epidermal LC in situ and is down-regulated upon culture. A subset of cells also expresses mLangerin in bone marrow cultures supplemented with TGF-beta. Notably, dendritic cells in thymic medulla are mLangerin-positive. By contrast, only scattered cells express mLangerin in lymph nodes and spleen. mLangerin mRNA is also detected in some nonlymphoid tissues (e.g., lung, liver, and heart). Similarly to hLangerin, a network of BG form upon transfection of mLangerin cDNA into fibroblasts. Interestingly, substitution of a conserved residue (Phe(244) to Leu) within the carbohydrate recognition domain transforms the BG in transfectant cells into structures resembling cored tubules, previously described in mouse LC. Our findings should facilitate further characterization of mouse LC, and provide insight into a plasticity of dendritic cell organelles which may have important functional consequences. 相似文献
5.
Correspondence to: B. Frank. 相似文献
1