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排序方式: 共有73条查询结果,搜索用时 15 毫秒
1.
M H Sawaf A H Shabana A Pelissier N Forest J P Ouhayoun 《The International journal of developmental biology》1991,35(2):91-100
The characterization of cytokeratin (CK) in adult oral mucosa and developing teeth have been well documented in human. Cytokeratin distribution in developing oral mucosa has not yet been described. The aim of this study was to identify the expression of CK in human fetal tongue (week 10 to week 23) and to correlate the results with morphological maturation. Simple epithelial CK are expressed in all cell layers during the early stages, essentially in peridermal cells. From the 14th week, CK 18 is present only in the taste buds, making this polypeptide a reliable marker for this sensory organ. CK 4 and 13 are expressed from the 10th to the 23rd week by both ventral and dorsal lingual epithelia. Terminal differentiation keratins (CK 1, 2 and 10-11) can only be detected immunohistochemically at the 14th week in some cells on the external surface of some papillae. The number of these papillae and positive cells increase at the 19th and 23rd weeks. The terminal differentiation markers are expressed several weeks earlier than the formation of a well-distinguished keratinized layer. 相似文献
2.
T Pelissier H Saavedra D Bustamante C Paeile 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1989,92(2):319-322
1. Octodon degus shows higher levels of tolerance to morphine when compared with the Wistar rat. 2. In the formalin algesiometric test, this caviomorph is more resistant to pain (P less than 0.01) and to the analgesic effect of morphine (P less than 0.001). 3. CD50 and LD50 were significantly higher in Octodon degus as compared with Wistar rat. 4. Morphine caused in rat severe hypotension, while doses eight times higher in O. degus had a transient effect. 5. 3H-naloxone binding in adrenal glands of O. degus is higher than in other tissue samples assayed from the same animal or rats. 相似文献
3.
Changes in carbon storage in temperate humic loamy soils after forest clearing and continuous corn cropping in France 总被引:3,自引:0,他引:3
Soil samples from forest and agricultural sites in three areas of southwest France were collected to determine the effect
of forest conversion to continuous intensive corn cropping with no organic matter management on soil organic carbon (C) content.
Soils were humic loamy soils and site characteristics that may affect soil C were as uniform as possible (slope, elevation,
texture, soil type, vegetation).
Three areas were selected, with adjacent sites of various ages of cultivation (3 to 35 yr), and paired control forest sites.
The ploughed horizon (0-Dt cm) and the Dt-50 cm layer were collected at each agricultural site. In forest sites, each 10 cm
layer was collected systematically down to 1 meter depth. Carbon concentrations were converted to total content to a given
depth as the product of concentration, depth of sample and bulk density, and expressed in units of kg m-2. For each site and each sampled layer, the mineral mass of soil was calculated, in order to base comparisons on the same
soil mass rather than the same depth.
The pattern of C accumulation in forest soils showed an exponential decrease with depth. Results suggested that soil organic
carbon declined rapidly during the first years of cultivation, and at a slower rate thereafter. This pattern of decrease can
be fitted by a bi-exponential model assuming that initial soil organic carbon can be separated into two parts, a very labile
pool reduced during the first rapid decline and more refractory fractions oxidizing at a slower rate. Sampling to shallow
depths (0-Dt cm) resulted in over-estimation of the rate of carbon release in proportion to the initial amount of C, and in
under-estimation of the total loss of C with age. The results for the 0–50 cm horizon indicated that losses of total carbon
average about 50% in these soils, ranging in initial carbon content from 19 to 32.5 kg m-2. Carbon release to the atmosphere averaged 0.8 kg m-2 yr-1 to 50 cm depth during the first 10 years of cultivation. The results demonstrate that temperate soils may also be an important
source of atmospheric carbon, when they are initially high in carbon content and then cultivated intensively with no organic
matter management. 相似文献
4.
Trafficking through Rab11 endosomes is required for cellularization during Drosophila embryogenesis 总被引:4,自引:0,他引:4
BACKGROUND: Embryonic cleavage leads to the formation of an epithelial layer during development. In Drosophila, the process is specialized and called cellularization. The trafficking pathways that underlie this process and that are responsible for the mobilization of membrane pools, however, remain poorly understood. RESULTS: We provide functional evidence for the role of endocytic trafficking through Rab11 endosomes in remobilizing vesicular membrane pools to ensure lateral membrane growth. Part of the membrane stems from endocytosed apical material. Mutants in the endocytic regulators rab5 and shibire/dynamin inhibit basal-lateral membrane growth, and apical endocytosis is blocked in shibire mutants. In addition, shibire controls vesicular trafficking through Rab11-positive endosomes. In shibire mutants, the transmembrane protein Neurotactin follows the secretory pathway normally but is not properly inserted in the plasma membrane and accumulates instead in Rab11 subapical endosomes. Consistent with a direct role of shibire in vesicular trafficking through Rab11 endosomes, Shibire is enriched in this compartment. Moreover, we show by electron microscopy the large accumulation of intracellular coated pits on subapical endocytic structures in shibire mutants. Finally, we show that Rab11 is essential for membrane growth and invagination during cellularization. CONCLUSION: Together, the data show that endocytic trafficking is required for basal-lateral membrane growth during cellularization. We identify Rab11 endosomes as key trafficking intermediates that control vesicle exocytosis and membrane growth during cellularization. This pathway may be required in other morphogenetic processes characterized by the growth of a membrane domain. 相似文献
5.
Didiot MC Serafini S Pfeifer MJ King FJ Parker CN 《Journal of biomolecular screening》2011,16(7):786-793
High-throughput screening assays with multiple readouts enable one to monitor multiple assay parameters. By capturing as much information about the underlying biology as possible, the detection of true actives can be improved. This report describes an extension to standard luciferase reporter gene assays that enables multiple parameters to be monitored from each sample. The report describes multiplexing luciferase assays with an orthogonal readout monitoring cell viability using reduction of resazurin. In addition, this technical note shows that by using the luciferin substrate in live cells, an assay time course can be recorded. This enables the identification of nonactive or unspecific compounds that act by inhibiting luciferase, as well as compounds altering gene expression or cell growth. 相似文献
6.
Hennebert O Pelissier MA Le Mee S Wülfert E Morfin R 《The Journal of steroid biochemistry and molecular biology》2008,110(3-5):255-262
High dose levels of dehydroepiandrosterone and its 7-hydroxylated derivatives have been shown to reduce oxidative stress and inflammatory responses in dextran sodium sulfate (DSS)-induced colitis in rats. Another endogenous steroid, 7beta-hydroxy-epiandrosterone (7beta-hydroxy-EpiA) has been shown to exert neuroprotective effects at much smaller doses. Our aims were to evaluate whether 7beta-hydroxy-EpiA pre-treatment prevents DSS-induced colitis and to determine whether the effects involve changes in anti-inflammatory prostaglandin (PG) D(2) and 15-deoxy-Delta(12,14)-PGJ(2) (15d-PGJ(2)) levels. Rats were administered 0.01, 0.1 and 1mg/kg 7beta-hydroxy-EpiA i.p. once a day for 7 days. Thereafter, colitis was induced by administration of 5% DSS in drinking water for 7 days. Levels of the PGs and the expression of cyclooxygenase (COX-2) and PG synthases were assessed during the course of the experiment. Administration of 7beta-hydroxy-EpiA caused a transient increase in COX-2 and PGE synthase expression within 6-15h and augmented colonic tissue levels of 15d-PGJ(2) levels starting at day 2. Treatment with DSS resulted in shortened colon length, depleted mucus in goblet cells and induced oxidative stress. COX-2 and mPGES-1 synthase expression were enhanced and accompanied by increased PGE(2), D(2) and 15d-PGJ(2) production. Although all dose levels of 7beta-hydroxy-EpiA reduced PGE(2) production, only the lowest dose (0.01mg/kg) of the steroid completely prevented colitis damage and tissue inflammation. 7beta-Hydroxy-EpiA pre-treatment prevents the occurrence of DSS-induced colitis through a shift from PGE(2) to PGD(2) production, associated with an early but transient increase in COX-2 expression and a sustained increase in the production of the anti-inflammatory prostaglandin 15d-PGJ(2). 相似文献
7.
Julien Paolini Alessandra Falchi Yann Quilichini Jean-Marie Desjobert Marie-Cecile De Cian Laurent Varesi Jean Costa 《Phytochemistry》2009,70(9):1146-1160
Cistus creticus L., an aromatic species from the Mediterranean area, contains various diterpenes bearing the labdane skeleton. The production of essential oil from this species has potential economic value, but so far, it has not been optimized. In order to contribute to a better knowledge of this species and to its differentiation, the morphological characters, volatile chemical composition and genetic data of two subspecies (C. creticus subsp. eriocephalus and C. creticus subsp. corsicus) were investigated. The leaf trichomes were studied using scanning electron microscopy. The chemical composition of Corsican essential oil (C. creticus subsp. corsicus) has been reported using GC, GC/MS and 13C NMR; the main constituents were oxygenated labdane diterpenes (33.9%) such as 13-epi-manoyl oxide (18.5%). Using plant material (54 samples) collected from 18 geographically distinct areas of the islands of Corsica and Sardinia, the basis of variation in the headspace solid-phase microextraction volatile fraction and an inter-simple sequence repeat genetic analysis were also examined. It was shown that the two subspecies of C. creticus differed in morphology, essential oil production, volatile fraction composition and genetic data. 相似文献
8.
Luis Constandil Alejandro Hernández Teresa Pelissier Osvaldo Arriagada Karla Espinoza Hector Burgos Claudio Laurido 《Arthritis research & therapy》2009,11(4):R105-9
Introduction
Cytokines produced by spinal cord glia after peripheral injuries have a relevant role in the maintenance of pain states. Thus, while IL-1β is overexpressed in the spinal cords of animals submitted to experimental arthritis and other chronic pain models, intrathecal administration of IL-1β to healthy animals induces hyperalgesia and allodynia and enhances wind-up activity in dorsal horn neurons. 相似文献9.
The stability of a plastid transgene has been evaluated in soybean transformants over six generations. These transformants
had integrated the aadA selection cassette in the intergenic region between the rps12/7 and trnV genes. Three independent homoplasmic T0 transformation events were selected and ten plants from each event propagated to
generation T5 in the absence of selection pressure. No transgene rearrangement nor wild-type plastome were detected in generation
T5 by Southern blot analysis. All tested progenies were uniformly resistant to spectinomycin. Therefore, soybean transformants
of generations T0 and T5 appear to be genetically and phenotypically identical. 相似文献
10.
Kienitz MC Bender K Dermietzel R Pott L Zoidl G 《The Journal of biological chemistry》2011,286(1):290-298
A large conductance (~300 picosiemens) channel (LCC) of unknown molecular identity, activated by Ca(2+) release from the sarcoplasmic reticulum, particularly when augmented by caffeine, has been described previously in isolated cardiac myocytes. A potential candidate for this channel is pannexin 1 (Panx1), which has been shown to form large ion channels when expressed in Xenopus oocytes and mammalian cells. Panx1 function is implicated in ATP-mediated auto-/paracrine signaling, and a crucial role in several cell death pathways has been suggested. Here, we demonstrate that after culturing for 4 days LCC activity is no longer detected in myocytes but can be rescued by adenoviral gene transfer of Panx1. Endogenous LCCs and those related to expression of Panx1 share key pharmacological properties previously used for identifying and characterizing Panx1 channels. These data demonstrate that Panx1 constitutes the LCC of cardiac myocytes. Sporadic openings of single Panx1 channels in the absence of Ca(2+) release can trigger action potentials, suggesting that Panx1 channels potentially promote arrhythmogenic activities. 相似文献