CRISPR/Cas9-mediated gfp gene inactivation in Arabidopsis suspension cells

Molecular Biology Reports - Targeted genome editing using CRISPR/Cas9 is a promising technology successfully verified in various plant species; however, it has hardly been used in plant cell...     

Mosaic patterns of transgene expression in plants

The review describes the phenomenon of mosaic transgene (gene) expression in plants. Parallels with the mosaic transgene (gene) expression in other organisms are presented. Parallels with the mosaic patterns of gene (transgene) expression in other organisms (Drosophila, transgenic animals, and others) are made.     

Peculiarities in Creation of Genetic Engineering Constructions for Knock-In Variant of Genome Editing of Arabidopsis thaliana Cell Culture

Russian Journal of Plant Physiology - The peculiarities in the creation of genetic engineering tools for the knock-in variant of genome editing are considered in detail on the example of gfp gene...     

The effect of duplications in the T-DNA on the stability of manifestation of heterologous genes in transgenic tobacco plants

The duplication of uidA gene within T-DNA was shown to disturb stability of expression of another marker gene, nptII, in the second generation (T2) of selfed initial transformants and in F1 hybrids of the crosses with nontransgenic tobacco. Hybridological analysis of the progeny resulting from various crosses involving T1 plants demonstrated that the expression of nptII gene was impaired in the hybrids that were hemizygous for the inactivated copy of uidA gene.     

The effect of duplications in the T-DNA on the stability of manifestation of heterologous genes in transgenic tobacco plants

The duplication of uidA gene within T-DNA was shown to disturb stability of expression of another marker gene, nptII, in the second generation (T₂) of selfed initial transformants and in F₁ hybrids of the crosses with nontransgenic tobacco. Hybridological analysis of the progeny resulting from various crosses involving T₁ plants demonstrated that the expression of nptII gene was impaired in the hybrids that were hemizygous for the inactivated copy of uidA gene.     

Mosaic expression pattern of the <Emphasis Type="Italic">nptII</Emphasis> gene in transgenic tobacco plants Nu 21

Mosaic expression pattern of the nptII gene in transgenic tobacco Nu 21 leaf somatic cells was demonstrated. Inheritance of this phenotype (in T₁–T₄ and F₁ backcrosses) was revealed. Three plant groups were distinguished, with low frequency of variegation manifestation (0–21.8%), with the high frequency of mosaic progeny (63.1 to 100%), and the intermediate type, where the frequency of the appearance of mosaic plants varied in a wide range, from 0 to 100%. The data obtained suggested the existence of two metastable states of a transgene in the leaf disk somatic cells (active and silenced), which could be associated with DNA modification, i.e., methylation of cytosine within the nptII gene sequence.