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1.
I. Yu. Malyshev E. B. Manukhina V. D. Mikoyan L. N. Kubrina A. F. Vanin 《FEBS letters》1995,370(3):159
Heat shock potentiated the nitric oxide production (EPR assay) in the liver, kidney, heart, spleen, intestine, and brain. The heat shock-induced sharp transient increase in the rate of nitric oxide production preceded the accumulation of heat shock proteins (HSP70) (Western blot analysis) as measured in the heart and liver. In all organs the nitric oxide formation was completely blocked by the NO-synthase inhibitor
(L-NNA). L-NNA also markedly attenuated the heat shock-induced accumulation of HSP70. The results suggests that nitric oxide is involved in the heat shock-induced activation of HSP70 synthesis. 相似文献
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Malyshev IYu Bayda LA Trifonov AI Larionov NP Kubrina LD Mikoyan VD Vanin AF Manukhina EB 《Physiological research / Academia Scientiarum Bohemoslovaca》2000,49(1):99-105
In this work, we evaluated the effect of adaptation to heat on the fall of blood pressure (BP) induced by heat shock (HS) and the interrelation between nitric oxide (NO) and heat shock protein, HSP70. Experiments were carried out on Wistar rats. It was shown that HS resulted in a generalized and transient increase in NO production (the electron paramagnetic resonance method) and a fall of BP from 113+/-3 to 88+/-1 mm Hg (p<0.05). Adaptation to heat itself did not affect BP, but completely prevented the NO overproduction and hypotension induced by HS. The adaptation simultaneously increased the brain NO-synthase content and induced HSP70 synthesis (the Western blot analysis) in various organs. Both the antihypotensive effects of adaptation and HSP70 accumulation were completely prevented by L-NNA, an inhibitor of NO synthesis, or quercetin, an inhibitor of HSP70 synthesis. The data suggest that adaptation to heat stimulates NO synthesis and NO activates synthesis of HSP70. HSP70, which hampers NO overproduction, thus restricts the BP fall induced by heat shock. 相似文献
4.
Pokidyshev DA Bondarenko NA Malyshev IIu Mikoian VD Kubrina LN Vanin AF Manukhina EB 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》1998,84(12):1420-1427
The study has shown that Nw-nitro-L-arginine, a nonselective nitric oxide (NO) inhibitor, in low non-vasoactive doses (10 mg/kg) exerted a protective effect in heat shock as demonstrated by a decrease in the mortality rate and prevention of acute hypotension in rats. The L-NNA in the same dose inhibited the basal NO production but left unaffected a carbachol-activated NO production. The findings suggest a possibility in principle of preferential inhibition of inducible NO-synthase in pathological conditions related to the NO overproduction using non-vasoactive doses of L-NNA the nonselective NO-synthase inhibitor. 相似文献
5.
E. B. Manukhina B. V. Smirin I. Yu. Malyshev J.-C. Stoclet B. Muller A. P. Solodkov V. I. Shebeko A. F. Vanin 《Biology Bulletin》2002,29(5):477-486
Nitric oxide (NO) is a highly reactive substance with short lifetime. In conditions of a living organism NO can be bound by the complexes used for transport and intracellular storage of NO. The main biological forms of NO store include S-nitrosothiols and dinitrosyl iron complexes capable of interconversion. The NO store formed by these complexes in the vascular wall, on the one hand, provides for protection from excessive free NO after its overproduction and, on the other hand, can be an additional NO source when it is deficient. Apparently, the efficiency of NO storage is genetically determined and corresponds to the inherited level of NO production in the organism. Controlled modulation of formation and dissociation of the NO store is a promising trend for further investigation. 相似文献
6.
F Z Meerson E B Manukhina A V Lapshin E E Ustinova 《Biulleten' eksperimental'no? biologii i meditsiny》1989,108(7):21-24
The effect of experimental myocardial infarction on endothelium-dependent relaxation was studied on isolated rat aorta and compared with the dynamics of arterial pressure (AP). It was shown that the endothelium-dependent relaxation of aorta was increased 1.8 times 3 h following the myocardial infarction. Simultaneously the drop in AP which had begun immediately following the experimental infarction became maximal. In 24 h both the indices were restored practically to the initial level. There was a significant negative correlation between the extent of endothelium-dependent relaxation and AP. It was suggested that the increase in endothelium-dependent relaxation could influence vascular tone, the drop in AP, and, finally, the development of cardiogenic shock in myocardial infarction in man. 相似文献
7.
V G Pinelis E B Manukhina Kh M Markov 《Biulleten' eksperimental'no? biologii i meditsiny》1987,103(2):151-154
The emotional stress decreased contractility and adrenoreactivity in normotensive rats, while in spontaneously hypertensive rats (SHR) the changes were less pronounced. The changes in adrenoreactivity, as well as increased permeability of smooth muscle cells for calcium and reduced reactivity to exogenous calcium revealed in hypertensive animals contribute to high resistance of portal vein smooth muscles to stress. 相似文献
8.
F Z Meerson L S Katkova Iu P Kozlov E B Manukhina 《Biulleten' eksperimental'no? biologii i meditsiny》1983,96(12):25-28
Studies of contractile function of an isolated right auricle in Wistar rats have demonstrated that long-term immobilization stress (fixation in the lying position for 6 h) results in the decreased extensibility of the auricle and pronounced depression of the developed tension. Preliminary adaptation of the animals to short-term immobilization stress (daily fixation in the lying position for 1 h over 10 days) per se insignificantly affects the extensibility and contractile function of the auricle but in effect it reduces its adrenoreactivity and completely prevents the post-stressor rigidity of the auricle and its function abnormality after long-term stress. 相似文献
9.
Ryou MG Sun J Oguayo KN Manukhina EB Downey HF Mallet RT 《Experimental biology and medicine (Maywood, N.J.)》2008,233(6):766-774
Physiologically modulated concentrations of nitric oxide (NO) are generally beneficial, but excessive NO can injure myocardium by producing cytotoxic peroxynitrite. Recently we reported that intermittent, normobaric hypoxia conditioning (IHC) produced robust cardioprotection against infarction and lethal arrhythmias in a canine model of coronary occlusion-reperfusion. This study tested the hypothesis that IHC suppresses myocardial nitric oxide synthase (NOS) activity and thereby dampens explosive, excessive NO formation upon reperfusion of occluded coronary arteries. Mongrel dogs were conditioned by a 20 d program of IHC (FIO(2) 9.5-10%; 5-10 min hypoxia/cycle, 5-8 cycles/d with intervening 4 min normoxia). One day later, ventricular myocardium was sampled for NOS activity assays, and immunoblot detection of the endothelial NOS isoform (eNOS). In separate experiments, myocardial nitrite (NO(2)(-)) release, an index of NO formation, was measured at baseline and during reperfusion following 1 h occlusion of the left anterior descending coronary artery (LAD). Values in IHC dogs were compared with respective values in non-conditioned, control dogs. IHC lowered left and right ventricular NOS activities by 60%, from 100-115 to 40-45 mU/g protein (P < 0.01), and decreased eNOS content by 30% (P < 0.05). IHC dampened cumulative NO(2)(-) release during the first 5 min reperfusion from 32 +/- 7 to 14 +/- 2 mumol/g (P < 0.05), but did not alter hyperemic LAD flow (15 +/- 2 vs. 13 +/- 2 ml/g). Thus, IHC suppressed myocardial NOS activity, eNOS content, and excessive NO formation upon reperfusion without compromising reactive hyperemia. Attenuation of the NOS/NO system may contribute to IHC-induced protection of myocardium from ischemia-reperfusion injury. 相似文献