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Galina A. Korshunova Eugene Y. Krynetski Manana T. Mtchedlidze Denis V. Agapkin William E. Evans Natalia F. Krynetskaia 《Nucleosides, nucleotides & nucleic acids》2013,32(6-7):1747-1748
Abstract Synthesis of a number of photoactive thiopurine-containing nucleosides was described. S-methylation of the synthesized compounds in the course of the reaction catalyzed by recombinant human thiopurine S-methyltransferase was studied by UV-spectroscopy. 相似文献
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Zenderland Jasmine Hart Robbie Bussmann Rainer W. Paniagua Zambrana Narel Y. Sikharulidze Shalva Kikvidze Zaal Kikodze David Tchelidze David Khutsishvili Manana Batsatsashvili Ketevan 《Economic botany》2019,73(3):293-303
Economic Botany - The Use of “Use Value”: Quantifying Importance in Ethnobotany. Use value (UV) is an index widely used to quantify the relative importance of useful plants. It combines... 相似文献
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Human O6-alkylguanine-DNA alkyltransferase (AGT) repairs mutagenic O6-alkylguanine and O4-alkylthymine adducts in single-stranded and duplex DNAs. These activities protect normal cells and tumor cells against drugs that alkylate DNA; drugs that inactivate AGT are under test as chemotherapeutic enhancers. In studies using 6-carboxyfluorescein (FAM)-labeled DNAs, AGT reduced the fluorescence intensity by ∼40% at binding saturation, whether the FAM was located at the 5′ or the 3′ end of the DNA. AGT protected residual fluorescence from quenching, indicating a solute-inaccessible binding site for FAM. Sedimentation equilibrium analyses showed that saturating AGT-stoichiometries were higher with FAM-labeled DNAs than with unlabeled DNAs, suggesting that the FAM provides a protein binding site that is not present in unlabeled DNAs. Additional fluorescence and sedimentation measurements showed that AGT forms a 1:1 complex with free FAM. Active site benzylation experiments and docking calculations support models in which the primary binding site is located in or near the active site of the enzyme. Electrophoretic analyses show that FAM inhibits DNA binding (IC50 ∼ 76 μM) and repair of DNA containing an O6-methylguanine residue (IC50 ∼ 63 μM). Similar results were obtained with other polycyclic aromatic compounds. These observations demonstrate the existence of a new class of non-covalent AGT-inhibitors. After optimization for binding-affinity, members of this class might be useful in cancer chemotherapy. 相似文献
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Olivares A Buadze M Kutubidze T Lobjanidze M Labauri L Kutubidze R Chikviladze D Zhvania M Kharzeishvili O Lomidze N Perez-Perez GI 《Helicobacter》2006,11(2):81-85
BACKGROUND: Georgia has showed a high prevalence of peptic ulcer disease (PUD), but the prevalence of Helicobacter pylori in this country is practically unknown. The purpose of this study was to determine the prevalence of H. pylori and specific genotypes in different populations in Georgia. MATERIALS AND METHODS: We studied 62 patients from several hospitals in Tbilisi, Georgia. More than 55% of patients had PUD. We determined H. pylori presence as well as specific genotypes cagA and vacA by polymerase chain reaction. In addition, we studied serum samples from 94 healthy persons to determine H. pylori and CagA prevalence by ELISA. RESULTS: We found a high prevalence of H. pylori and CagA in the healthy population (70.2 and 57.4%, respectively) and a high prevalence of CagA among the H. pylori-positive persons (71.2%). Prevalence increased with age as reported in other countries (p = .05). Among symptomatic persons, we found nearly the same high prevalence of H. pylori (64.5%) as in the asymptomatic population. Furthermore, in symptomatic H. pylori patients, we found 65.0 and 67.5% prevalence of cagA and vacA, respectively. For 33 patients with PUD, 24 patients (72.7%) were H. pylori positive and 66.7% of them were cagA positive. In contrast, among the patients with non-ulcer dyspepsia (NUD), 16 (55.2%) were H. pylori positive and 62.5% of them were colonized with cagA-positive strains. H. pylori and cagA prevalence were not significantly different between PUD and patients with NUD. CONCLUSIONS: We confirmed that among individuals in Georgia, the prevalence of H. pylori is high and cagA-positive strains were equally present among H. pylori-positive patients with PUD and NUD and asymptomatic persons. 相似文献
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Maria Taranenko Anna Rykhlevskaya Manana Mtchedlidze Jacques Laval Svetlana Kuznetsova 《European journal of biochemistry》2003,270(14):2945-2949
Formamidopyrimidine-DNA glycosylase (Fpg protein) of Escherichia coli is a DNA repair enzyme that excises oxidized purine bases, most notably the mutagenic 7-hydro-8-oxoguanine, from damaged DNA. In order to identify specific contacts between nucleobases of DNA and amino acids from the E. coli Fpg protein, photochemical cross-linking was employed using new reactive DNA duplexes containing 5-[4-[3-(trifluoromethyl)-3H-diazirin-3-yl]phenyl]-2'-deoxyuridine dU* residues near the 7-hydro-8-oxoguanosine (oxoG) lesion. The Fpg protein was found to bind specifically and tightly to the modified DNA duplexes and to incise them. The nicking efficiency of the DNA duplex containing a dU* residue 5' to the oxoG was higher as compared to oxidized native DNA. The conditions for the photochemical cross-linking of the reactive DNA duplexes and the Fpg protein have been optimized to yield as high as 10% of the cross-linked product. Our results suggest that the Fpg protein forms contacts with two nucleosides, one 5' adjacent to oxoG and the other 5' adjacent to the cytidine residue pairing with oxoG in the other strand. The approaches developed may be applicable to pro- and eukaryotic homologues of the E. coli Fpg protein as well as to other repair enzymes. 相似文献
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Kotrikadze N. G. Khutsishvili E. G. Chelidze M. Z. Alibegashvili M. R. Artsyvadze K. L. Sanikidze T. V. Managadze L. G. Chigogidze T. G. 《Biophysics》2011,56(4):717-721
Paramagnetic centers in the blood of patients with prostate adenocarcinoma have been studied prior to and after plastic orchectomy
by EPR. It has been shown that significant alterations in blood paramagnetic centers occur after the surgery. These alterations
point to an improvement of antioxidant properties of the blood. Specifically, the content of the Fe3+-transferrin complexes increased, and the number of inactivated adrenoreceptors, MetHb, and Mn2+-containing complexes decreased. It was found that, along with improvement of the antioxidant properties of the blood, a significant
decrease in the intensity of lipid peroxidation in the blood of the patients occurs. 相似文献
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Giorgobiani E Chitadze N Chanturya G Grdzelidze M Jochim RC Machablishvili A Tushishvili T Zedginidze Y Manjgaladze MK Iashvili N Makharadze MP Zakaraya T Kikaleishvili K Markhvashvili I Badashvili G Daraselia T Fay MP Kamhawi S Sacks D 《PLoS neglected tropical diseases》2011,5(12):e1415