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F Mami J L Guenet R Perret P Truffa-Bachi 《Journal of immunology (Baltimore, Md. : 1950)》1986,136(12):4432-4437
IgD and IgM are concomitantly expressed on the surface of most mouse B lymphocytes and both molecules serve as receptor for antigen. In this communication we report that in contrast to IgM, which is expressed in a constant manner on the surface of spleen B lymphocytes of different laboratory and wild-derived mice, IgD expression is variable among the spleen cells of wild-derived mice. SPE, SEI, and SFM mice belonging to the Mus 3 subgroup show a fluorescence profile characterized by a marked diminution in the population of B lymphocytes expressing the IgD isotype; in addition, these cells have a low sIgD density on their membranes. These findings were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the iodinated membrane proteins. Polyclonal in vitro activation with lipopolysaccharide increases the frequency of surface IgD (sIgD)-bearing spleen cells and sIgD density in the SPE strain but decreases both the frequency and the density of IgD bearing cells in the BALB/c strain. This result suggests that delta gene expression is regulated differently in SPE and BALB/c mice. In addition, genetic analysis of sIgD expression in (BALB/c X SPE)F1 hybrids suggests that the proportion of sIgD-bearing cells and sIgD density are independently regulated. 相似文献
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Scymnodalatias albicauda sp. nov. is described from two specimens taken at high latitudes (45°S and 49°S). It is distinguished fromS. sherwoodi, only known species of the genus, by having white markings on the caudal fin, the second dorsal posterior tip almost reaching the upper caudal fin, shorter snout and head, smaller eye and larger fins. Relationships ofScymnodalatias to the generaScymnodon, Centroscymnus, andZameus are discussed, based chiefly on dermal denticle structure.Scymnodalatias andZameus uniquely share transverse ridges on their dermal denticles, and on this character they are treated as sister-groups. Comments on the above genera,Z. squamulosus and some species ofScymnodon are made to clarify their systematic status. As a result, it is proposed thatScymnodon includesichiharai, macracanthus, plunketi, andringens, thatCentroscymnus includescoelolepis, crepidater, crypt acanthus, andowstonii, and thatZameus includessquamulosus. 相似文献
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On the specificity of cytochrome c synthetase in recognition of the amino acid sequence of apocytochrome c 总被引:2,自引:0,他引:2
Two forms of yeast cytochrome c synthetases with different specificities were resolved, one (synthetase I), solubilized from mitochondria or the cell debris with Triton X-100, recognizing not horse apocytochrome c but yeast apo-iso-1-cytochrome c as a substrate and the other (synthetase II) still bound with the particulate fraction from mitochondria after treatment with Triton, recognizing both horse and yeast apocytochromes c. The activity with labeled yeast apo-iso-1-cytochrome c as a substrate of cytochrome c synthetase I can be quantitatively inhibited by nonlabeled Candida krusei apocytochrome c and partially by nonlabeled tuna apocytochrome c but not by nonlabeled horse apocytochrome c indicating a specific amino acid sequence being recognized. However, an enzyme similarly solubilized from beef heart mitochondria recognized both horse apocytochrome c and yeast apo-iso-1-cytochrome c for attachment of heme. In view of the fact that the yeast synthetase II and the beef synthetase can both utilize either horse apocytochrome c or yeast apo-iso-1-cytochrome c as substrates, we suggest that these enzymes may also be involved in biosynthesis of cytochrome c1, that is, the ability to attach heme to apocytochrome c and apocytochrome c1 may have been conserved in eucaryotic cells, and that both synthetases may therefore be homologous. 相似文献
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Mami Ohsugi Hideki Yamamura Reiji Semba Hiroyoshi Hidaka 《The Histochemical journal》1994,26(8):641-643
Summary To confirm the possibility that protein kinase C is involved in compaction of mouse embryos, the presence and distribution pattern of Ca2+-dependent subspecies of this enzyme in mouse embryos, before and during compaction, were examined immunocytochemically with three different monoclonal antibodies. These were MC-1a, MC-2a and MC-3a, which selectively interact with the subspecies of the enzyme known as types I, II and III, respectively. Only when embryos were incubated with MC-3a, was immunofluorescence clearly detected in all cells of embryos before and during compaction. This result demonstrates the presence of type III protein kinase C in embryos before and during compaction and suggests the possibility that the type III enzyme may be involved in compaction. No marked differences were found in the distribution pattern of the type III enzyme between embryos examined before and during compaction. 相似文献
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