全文获取类型
收费全文 | 271篇 |
免费 | 25篇 |
出版年
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 11篇 |
2014年 | 9篇 |
2013年 | 30篇 |
2012年 | 9篇 |
2011年 | 17篇 |
2010年 | 7篇 |
2009年 | 10篇 |
2008年 | 15篇 |
2007年 | 8篇 |
2006年 | 13篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 10篇 |
2002年 | 7篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1969年 | 3篇 |
1968年 | 2篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1964年 | 5篇 |
1963年 | 1篇 |
排序方式: 共有296条查询结果,搜索用时 31 毫秒
1.
2.
Immobilisation of limbs in kittens, in which the neuromuscular apparatus is in active growing phase, affects the maturation of the end-plates, which appear smaller and pointed. Longer periods of immobilisation lead to a significant reduction in the surface area of the neuromuscular junctions which were seen to be "V" or "W" shaped. 相似文献
3.
Malathi Chari K. Gupta T. G. Prasad K. S. Krishna Sastry M. Udaya Kumar 《Plant and Soil》1986,91(1):109-114
Summary The effect of calcium in the water relations and tolerance to moisture deficits was tested in groundnut and cowpea. In both species, enrichment of tissue with calcium resulted in maintenance of a higher water status under stress associated with low proline accumulation. The extent of membrane damage (as reflected by the absorbance at 273 nm) was lesser in leaves of plants fed with higher levels of Ca++ when subjected to simulated stress. The rate of water loss from the leaves of Ca++-enriched plants was also lower. The possible role of Ca++ in inducing membrane stability and maintenance of higher water status is discussed. 相似文献
4.
We have characterized the biosynthesis of two metalloproteinases, procollagenase and prostromelysin, by rabbit brain capillary endothelial cells (RBCE) by means of immunochemical, biosynthetic, and functional assays. Unstimulated RBCE secreted no detectable metalloproteinases. Secretion of both procollagenase and prostromelysin was induced within 6 h by treating the cells with 50 ng/ml 12-O-tetradecanoylphorbol-13-acetate. In treated cells, the two proenzymes accounted for up to 20% of the [35S]methionine-labeled secreted proteins; about 15 micrograms of each protein was secreted in 48 h by 10(6) RBCE. Although RBCE secreted approximately as much procollagenase and prostromelysin as did rabbit fibroblasts, virtually no enzyme activity could be measured in RBCE-conditioned medium, even after activation of the proenzymes by trypsin or an organomercurial agent. 相似文献
5.
Rabbit kidney brush-border membrane vesicles were exposed to bacterial protease which cleaves off a large number of externally oriented proteins. Na+-dependent d-glucose transport is left intact in the protease-treated vesicles. The protease-treated membrane was solubilized with deoxycholate and the deoxycholate-extracted proteins were further resolved by passage through Con A-Sepharose columns. Sodium-dependent d-glucose activity was found to reside in a fraction containing a single protein band of Mr ? 165000 which is apparently a dimer of Mr ? 85 000. When reconstituted and tested for transport, this protein showed Na+-dependent, stereo-specific and phlorizin-inhibitable glucose transport. Transport activity is completely recovered and is 20-fold increased in specific activity. A similar isolate was obtained from rabbit small intestinal brush-border membranes and kidneys from several other species of animals. 相似文献
6.
7.
8.
Mouse macrophage elastase. Purification and characterization as a metalloproteinase. 总被引:28,自引:2,他引:26
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Macrophage elastase was purified from tissue-culture medium conditioned by inflammatory mouse peritoneal macrophages. Characterized as a secreted neutral metalloproteinase, this enzyme was shown to be catalytically and immunochemically distinct from the mouse pancreatic and mouse granulocyte elastases, both of which are serine proteinases. Inhibition profiles, production of nascent N-terminal leucine residues and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of degraded elastin indicated that macrophage elastase is an endopeptidase, with properties of a metalloproteinase, rather than a serine proteinase. Macrophage elastase was inhibited by alpha 2-macroglobulin, but not by alpha 1-proteinase inhibitor. Macrophage elastase was resolved into three chromatographically distinct forms. The predominant form had mol.wt. 22 000 and was purified 4100-fold. Purification of biosynthetically radiolabelled elastase indicated that this form represented less than 0.5% of the secreted protein of macrophages. Approx. 800% of the starting activity was recovered after purification. Evidence was obtained for an excess of an endogenous inhibitor masking more than 80% of the secreted activity. 相似文献
9.
Vibha Gupta G. LakshmiSita M. S. Shaila V. Jagannathan Malathi S. Lakshmikumaran 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(3-4):397-402
Summary The construction and characterization of two genome-specific recombinant DNA clones from B. nigra are described. Southern analysis showed that the two clones belong to a dispersed repeat family. They differ from each other in their length, distribution and sequence, though the average GC content is nearly the same (45%). These B genome-specific repeats have been used to analyse the phylogenetic relationships between cultivated and wild species of the family Brassicaceae. 相似文献
10.