排序方式: 共有57条查询结果,搜索用时 31 毫秒
1.
L Cima A Malacrida G Gasperi L Sacchi A Grigolo 《The journal of histochemistry and cytochemistry》1978,26(10):846-854
Single newly emerged males of Musca domestica, WHO strain, usually show five electrophoretic bands of glucose 6-phosphate dehydrogenase (G6PD) activity. Of these five molecular forms, designated with Roman numerals in order from the origin, we have considered the first three: these have been characterized with respect to their substrate and coenzyme specificity and to their sensitivity to some sulfhydryl inhibitors. The data show band III to be G6P specific, nicotinamide adenine dinucleotide phosphate dependent and to be a type I enzyme according to Kamada and Hori's classification. Bands I and II, on the other hand, show wide substrate specificity and low sensitivity to the sulfhydryl inhibitors assayed. In addition, in the absence of an exogenous substrate and in the presence of nicotinamide adenine dinucleotide as a coenzyme, fairly weak bands, which can be ascribed to the so called "nothing dehydrogenase" effect, are seen in the position I and II. Nevertheless, the data reported do not allow a clear definition of the enzymatic type corresponding to bands I and II of G6PD activity. 相似文献
2.
The phytophagous insects of the Tephritidae family commonly referred to as “true fruit flies” offer different case histories
of successful invasions. Mankind has played an important role in altering the distributions of some of the more polyphagous
and oligophagous species. However, the question arises why only a few species have become major invaders. The understanding
of traits underlying adaptation in different environments is a major topic in invasion biology. Being generalists or specialists,
along the K–r gradient of the growth curve, make a difference in term of food resources exploitation and interspecies competition and displacement.
The species of the genus Ceratitis are good examples of r-strategists. The genetic and biological data of the most notorious Ceratitis species, the Mediterranean fruit fly Ceratitis capitata (medfly), are reviewed to investigate the traits and behaviours that make the medfly an important invader. It can be learnt
from medfly, that invasions in a modern global trade network tend to be due to multiple introductions. This fact allows a
maintenance or enhancement of genetic variability in the adventive populations, which in turn increases their potential invasiveness.
Our current knowledge of the medfly genome opens the way for future studies on functional genomics. 相似文献
3.
Cristina Torti Ludvik M. Gomulski Anna R. Malacrida Pierre Capy Giuliano Gasperi 《Journal of molecular evolution》1998,46(3):288-298
Mariner elements were amplified using the polymerase chain reaction from two species of tephritid flies, Ceratitis rosa and Trirhithrum coffeae. The sequences were ∼1.3 kb in length. None of these elements appeared to be functional, as in every case the open reading
frame (ORF) was disrupted by the presence of frameshifts or stop codons. These elements, Crmar1 and Tcmar1, are very similar to the Ccmar1 element previously amplified from the closely related tephritid species C. capitata and are members of the mellifera subfamily of mariner elements. The phylogeny and pattern of divergence of these elements were examined in relation to the phylogeny of the host
species. It is highly probable that the elements were present in the ancestral lineage prior to the divergence of the three
species. The copy numbers of the elements within each species are very different, ranging from about 10 in T. coffeae to 5,000 in C. rosa. The possible mechanisms which determine the copy number of an element in the host genome are discussed.
Received: 25 April 1997 / Accepted: 31 July 1997 相似文献
4.
Sabrina Bertin Francesca Scolari Carmela R. Guglielmino Angelica Bonomi Ludvik M. Gomulski Anna R. Malacrida Carlo Matessi 《Journal of insect physiology》2010,56(11):1542-1551
The medfly, Ceratitis capitata, is an invasive species in which polyandry, associated with sperm precedence, is a common behaviour in the wild. In this species, characterized by internal fertilization, we disclose how the sperm from two males are stored in the female storage organs and how they are used in terms of paternity outcome. The experiments were designed to furnish comparable and unbiased estimates of sperm numbers and progeny in twice-mated females. Results are incorporated in a model through which it is possible to relate the amount of stored sperm with the progeny of twice-mated females. The results show that polyandrous medfly females conserve equal amounts of sperm from the two males to fertilize their eggs. However, we observed a clear advantage of the second male's sperm in siring progeny, which interestingly decreases in favor of the first male as ovipositions progress. The results enable us to exclude differential sperm mortality and suggest that it is the mechanics governing the storage organs which causes the initial, but decreasing second male sperm precedence during the female reproductive life. These outcomes allow us to correlate sperm use in polyandrous females with the mating strategies and invasiveness of this fly. 相似文献
5.
6.
Aketarawong N Chinvinijkul S Orankanok W Guglielmino CR Franz G Malacrida AR Thanaphum S 《Genetica》2011,139(1):129-140
The oriental fruit fly, Bactrocera dorsalis (Hendel), is a key pest that causes reduction of the crop yield within the international fruit market. Fruit flies have been
suppressed by two Area-Wide Integrated Pest Management programs in Thailand using Sterile Insect Technique (AW-IPM-SIT) since
the late 1980s and the early 2000s. The projects’ planning and evaluation usually rely on information from pest status, distribution,
and fruit infestation. However, the collected data sometimes does not provide enough detail to answer management queries and
public concerns, such as the long term sterilization efficacy of the released fruit fly, skepticism about insect migration
or gene flow across the buffer zone, and the re-colonisation possibility of the fruit fly population within the core area.
Established microsatellite DNA markers were used to generate population genetic data for the analysis of the fruit fly sampling
from several control areas, and non-target areas, as well as the mass-rearing facility. The results suggested limited gene
flow (m < 0.100) across the buffer zones between the flies in the control areas and flies captured outside. In addition, no genetic
admixture was revealed from the mass-reared colony flies from the flies within the control area, which supports the effectiveness
of SIT. The control pests were suppressed to low density and showed weak bottleneck footprints although they still acquired
a high degree of genetic variation. Potential pest resurgence from fragmented micro-habitats in mixed fruit orchards rather
than pest incursion across the buffer zone has been proposed. Therefore, a suitable pest control effort, such as the SIT program,
should concentrate on the hidden refuges within the target area. 相似文献
7.
Francesca Scolari Boaz Yuval Ludvik M Gomulski Marc F Schetelig Paolo Gabrieli Federico Bassetti Ernst A Wimmer Anna R Malacrida Giuliano Gasperi 《BMC genetics》2014,15(Z2):S10
Background
In the Mediterranean fruit fly (medfly), Ceratitis capitata, a highly invasive agricultural pest species, polyandry, associated with sperm precedence, is a recurrent behaviour in the wild. The absence of tools for the unambiguous discrimination between competing sperm from different males in the complex female reproductive tract has strongly limited the understanding of mechanisms controlling sperm dynamics and use.Results
Here we use transgenic medfly lines expressing green or red fluorescent proteins in the spermatozoa, which can be easily observed and unambiguously differentiated within the female fertilization chamber. In twice-mated females, one day after the second mating, sperm from the first male appeared to be homogenously distributed all over the distal portion of each alveolus within the fertilization chamber, whereas sperm from the second male were clearly concentrated in the central portion of each alveolus. This distinct stratified sperm distribution was not maintained over time, as green and red sperm appeared homogeneously mixed seven days after the second mating. This dynamic sperm storage pattern is mirrored by the paternal contribution in the progeny of twice-mated females.Conclusions
Polyandrous medfly females, unlike Drosophila, conserve sperm from two different mates to fertilize their eggs. From an evolutionary point of view, the storage of sperm in a stratified pattern by medfly females may initially favour the fresher ejaculate from the second male. However, as the second male's sperm gradually becomes depleted, the sperm from the first male becomes increasingly available for fertilization. The accumulation of sperm from different males will increase the overall genetic variability of the offspring and will ultimately affect the effective population size. From an applicative point of view, the dynamics of sperm storage and their temporal use by a polyandrous female may have an impact on the Sterile Insect Technique (SIT). Indeed, even if the female's last mate is sterile, an increasing proportion of sperm from a previous mating with a fertile male may contribute to sire viable progeny.8.
Corey Brelsfoard George Tsiamis Marco Falchetto Ludvik M. Gomulski Erich Telleria Uzma Alam Vangelis Doudoumis Francesca Scolari Joshua B. Benoit Martin Swain Peter Takac Anna R. Malacrida Kostas Bourtzis Serap Aksoy 《PLoS neglected tropical diseases》2014,8(4)
Tsetse flies (Glossina spp.) are the cyclical vectors of Trypanosoma spp., which are unicellular parasites responsible for multiple diseases, including nagana in livestock and sleeping sickness in humans in Africa. Glossina species, including Glossina morsitans morsitans (Gmm), for which the Whole Genome Sequence (WGS) is now available, have established symbiotic associations with three endosymbionts: Wigglesworthia glossinidia, Sodalis glossinidius and Wolbachia pipientis (Wolbachia). The presence of Wolbachia in both natural and laboratory populations of Glossina species, including the presence of horizontal gene transfer (HGT) events in a laboratory colony of Gmm, has already been shown. We herein report on the draft genome sequence of the cytoplasmic Wolbachia endosymbiont (cytWol) associated with Gmm. By in silico and molecular and cytogenetic analysis, we discovered and validated the presence of multiple insertions of Wolbachia (chrWol) in the host Gmm genome. We identified at least two large insertions of chrWol, 527,507 and 484,123 bp in size, from Gmm WGS data. Southern hybridizations confirmed the presence of Wolbachia insertions in Gmm genome, and FISH revealed multiple insertions located on the two sex chromosomes (X and Y), as well as on the supernumerary B-chromosomes. We compare the chrWol insertions to the cytWol draft genome in an attempt to clarify the evolutionary history of the HGT events. We discuss our findings in light of the evolution of Wolbachia infections in the tsetse fly and their potential impacts on the control of tsetse populations and trypanosomiasis. 相似文献
9.
Genetic and molecular investigations on the endogenous mobile elements of non-drosophilid fruitflies
A syndrome of abnormal genetic effects, resembling Drosophila hybrid dysgenesis, occurs in Ceratitis capitata when strains
of different origin are mated. The pattern of abnormal traits observed appears to be the phenotypic expression of a complex
interacting dysgenic system of inducer and suppressor effects; probably more than one system is activated in the crosses.
This suggests that different systems of mobile elements occur in different strains and populations of C. capitata. Using a
PCR primer specific to the ITR sequence of a deleted element, full length mariner elements were isolated from C. capitata,
Ceratitis rosa, and Trirhithrum coffeae. Very high similarities were found in inter- and intraspecific comparisons of the
elements. The majority of these elements contained deletions and frame-shifts. However, one clone Ccmar1.18, from C. capitata,
was found to possess an uninterrupted ORF coding for 338 amino acids with ∼60% similarity to the Mos1 element of Drosophila
mauritiana. Database searches and phylogenetic analyses showed that the mariner elements isolated in the present study are
representatives of Robertson's mellifera mariner subfamily. The copy numbers of the elements within each species are very
different, ranging from about 10 in T. coffeae to 5000 in C. rosa.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
10.
Bonizzoni M Katsoyannos BI Marguerie R Guglielmino CR Gasperi G Malacrida A Chapman T 《Molecular ecology》2002,11(10):1915-1921
Accurate estimates of remating in wild female insects are required for an understanding of the causes of variation in remating between individuals, populations and species. Such estimates are also of profound importance for major economic fruit pests such as the Mediterranean fruit fly (Ceratitis capitata). A major method for the suppression of this pest is the sterile insect technique (SIT), which relies on matings between mass-reared, sterilized males and wild females. Remating by wild females will thus impact negatively on the success of SIT. We used microsatellite markers to determine the level of remating in wild (field-collected) Mediterranean fruit fly females from the Greek Island of Chios. We compared the four locus microsatellite genotypes of these females and their offspring. Our data showed 7.1% of wild females remated. Skewed paternity among progeny arrays provided further evidence for double matings. Our lowest estimate of remating was 3.8% and the highest was 21%. 相似文献