Effects of diking on the biological performance of the black mangrove (Avicennia germinans L.) in an Atlantic mangrove forest

The effects of diking on the photosynthetic rate, gross glucose production, biomass, and nutrient levels of the black mangrove Avicennia germinans L., were investigated during the major dry, the major wet and the intermediate season in completely diked mangrove areas, and compared to partially diked and non-diked biotopes. In the major dry and major wet seasons, the photosynthetic rate and gross glucose production were two to five times lower in the completely diked sites compared to the partially diked and non-diked habitats. The average dry weights of branches, flowers and leaves did not differ significantly between sites. The same held true for propagules from the major dry and major wet season. However, propagules from the transition period collected in the non-diked and partially diked biotopes yielded appreciable amounts of dry matter, while plants in the completely diked forests did not carry any propagules. No statistically significant differences were found among nutrient content in the leaves of plants from the different habitats. Our results suggest black mangrove growing in diked habitats may suffer a certain degree of environmental stress, which may affect important biological processes, such as photosynthesis, gross glucose production, and propagule formation.     

Scleroderma-polymyositis overlap syndrome versus idiopathic polymyositis and systemic sclerosis: a descriptive study on clinical features and myopathology

Introduction

The objective was to characterize the clinical and myopathologic features of patients with scleroderma-polymyositis (SSc-PM) overlap compared with a population of patients with systemic sclerosis (SSc) and polymyositis (PM).

Methods

A three-way comparison of patients with SSc-PM overlap (n = 25) with patients with SSc (n = 397) and PM (n = 40) on clinical and myopathologic features and causes of death. One neuropathologist blinded for the diagnosis evaluated all recent available muscle biopsies. Biopsies were scored for presence of inflammation, necrotic muscle fibers, rimmed vacuoles, fibrosis, and immunohistochemical staining. Clinical or myopathologic characteristics were compared by using the χ² test or one-way analysis of variance (ANOVA).

Results

The prevalence of SSc-PM overlap in the Nijmegen Systemic Sclerosis cohort was 5.9%. The mortality was 32% (eight of 25) in SSc-PM, of which half was related to cardiac diseases. The prevalence of pulmonary fibrosis was significantly increased in SSc-PM (83%) (P = 0.04) compared with SSc (49%) and PM (53%). SSc or myositis-specific antibodies were nearly absent in the SSc-PM group. In almost all biopsies (96%) of SSc-PM patients, necrotic muscle fibers were present, which was significantly increased compared with PM patients (P = 0.02).

Conclusions

Patients with SSc-PM have increased prevalence of pulmonary fibrosis and cardiac disease as the cause of death compared with patients with SSc and PM . In addition, we found that necrotizing muscle fibers with inflammation characterize SSc-PM overlap in muscle biopsies. Further research should focus on underlying mechanisms causing necrosis, inflammation, and fibrosis and their relation to pulmonary involvement and mortality in patients with SSc-PM overlap.