全文获取类型
收费全文 | 657篇 |
免费 | 52篇 |
国内免费 | 1篇 |
出版年
2024年 | 1篇 |
2023年 | 10篇 |
2022年 | 29篇 |
2021年 | 49篇 |
2020年 | 52篇 |
2019年 | 87篇 |
2018年 | 58篇 |
2017年 | 23篇 |
2016年 | 26篇 |
2015年 | 31篇 |
2014年 | 29篇 |
2013年 | 51篇 |
2012年 | 60篇 |
2011年 | 30篇 |
2010年 | 17篇 |
2009年 | 22篇 |
2008年 | 17篇 |
2007年 | 32篇 |
2006年 | 12篇 |
2005年 | 22篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 4篇 |
2001年 | 9篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1984年 | 1篇 |
排序方式: 共有710条查询结果,搜索用时 15 毫秒
1.
Mahsa Zahiri Maryam Babaei Khalil Abnous Seyed Mohammad Taghdisi Mohammad Ramezani Mona Alibolandi 《Journal of cellular physiology》2020,235(2):1036-1050
In this study, the chemical features of dendritic mesoporous silica nanoparticles (DMSNs) provided the opportunity to design a nanostructure with the capability to intelligently transport the payload to the tumor cells. In this regard, doxorubicin (DOX)-encapsulated DMSNs was electrostatically surface-coated with polycarboxylic acid dextran (PCAD) to provide biocompatible dextran-capped DMSNs (PCAD-DMSN@DOX) with controlled pH-dependent drug release. Moreover, a RNA aptamer against a cancer stem cell (CSC) marker, CD133 was covalently attached to the carboxyl groups of DEX to produce a CD133-PCAD-DMSN@DOX. Then, the fabricated nanosystem was utilized to efficiently deliver DOX to CD133+ colorectal cancer cells (HT29). The in vitro evaluation in terms of cellular uptake and cytotoxicity demonstrated that the CD133-PCAD-DMSN@DOX specifically targets HT29 as a CD133 overexpressed cancer cells confirmed by flow cytometry and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. The potentially promising intelligent-targeted platform suggests that targeted dextran-capped DMSNs may find impressive application in cancer therapy. 相似文献
2.
Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. 总被引:101,自引:0,他引:101
E Y Skolnik B Margolis M Mohammadi E Lowenstein R Fischer A Drepps A Ullrich J Schlessinger 《Cell》1991,65(1):83-90
A novel method has been developed to allow cloning of protein targets for receptors with tyrosine kinase activity. By utilizing the carboxy-terminal tail of EGF receptor (EGFR) as a probe to screen lambda gt11 expression libraries, several EGFR-binding proteins have been cloned; two have been analyzed and contain unique SH2 and SH3 domains. One gene (GRB-1) has been fully sequenced, is expressed in various tissues and cell lines, and has a molecular mass of 85 kd. Interestingly, GRB-1 encodes the human counterpart of the PI3 kinase-associated protein p85. Advantages of this technique include the ease of cloning tyrosine kinase receptor targets present at low levels and the ability to identify proteins that are related in their capacity to bind activated receptors but contain no significant DNA sequence homology. This method, termed CORT (for cloning of receptor targets), offers a general approach for the identification and cloning of various receptor targets. 相似文献
3.
Identification of six novel autophosphorylation sites on fibroblast growth factor receptor 1 and elucidation of their importance in receptor activation and signal transduction. 总被引:27,自引:6,他引:21
下载免费PDF全文
![点击此处可从《Molecular and cellular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
M Mohammadi I Dikic A Sorokin W H Burgess M Jaye J Schlessinger 《Molecular and cellular biology》1996,16(3):977-989
Fibroblast growth factor receptor (FGFR) activation leads to receptor autophosphorylation and increased tyrosine phosphorylation of several intra cellular proteins. We have previously shown that autophosphorylated tyrosine 766 in FGFR1 serves as a binding site for one of the SH2 domains of phospholipase Cy and couples FGFR1 to phosphatidylinositol hydrolysis in several cell types. In this report, we describe the identification of six additional autophosphorylation sites (Y-463, Y-583, Y-585, Y-653, Y-654 and Y-730) on FGFR1. We demonstrate that autophosphorylation on tyrosines 653 and 654 is important for activation of tyrosine kinase activity of FGFR1 and is therefore essential for FGFR1-mediated biological responses. In contrast, autophosphorylation of the remaining four tyrosines is dispensable for FGFR1-mediated mitogen-activated protein kinase activation and mitogenic signaling in L-6 cells as well as neuronal differentiation of PC12 cells. Interestingly, both the wild-type and a mutant FGFR1 (FGFR1-4F) are able to phosphorylate Shc and an unidentified Grb2-associated phosphoprotein of 90 kDa (pp90). Binding of the Grb2/Sos complex to phosphorylated Shc and pp90 may therefore be the key link between FGFR1 and the Ras signaling pathway, mito-genesis, and neuronal differentiation. 相似文献
4.
Plasma Physics Reports - So far, the detailed experimental effect of the inductance on the X-ray yield in the Filippov-type plasma focus devices has not been documented in literature. In this... 相似文献
5.
Plasma Physics Reports - The effects of different nuclear reactions on thermonuclear burn-up conditions of equimolar mixture of deuterium–tritium are investigated. The minimum requirements... 相似文献
6.
Mehrizadeh Vahid Dorani Ebrahim Mohammadi Seyed Abolghasem Ghareyazie Behzad 《Plant Cell, Tissue and Organ Culture》2021,145(1):127-141
Plant Cell, Tissue and Organ Culture (PCTOC) - Globular androgenic haploid embryos of TV21 and TV19 cultivars of Camellia ssp., obtained on embryo induction medium (EIM), Murashige and Skoog medium... 相似文献
7.
8.
9.
Mohsen Mohammadi Parva Dehghani Atefeh Mohseninia Mona Roozbehani Andrew Hemphill Khashayar Hesamizadeh 《Journal of cellular physiology》2021,236(2):1401-1417
A major challenge for the development of anticancer vaccines is the induction of a safe and effective immune response, particularly mediated by CD8+ T lymphocytes, in an adjuvant‐free manner. In this respect, we present a simple strategy to improve the specific CD8+ T cell responses using KFE8 nanofibers bearing a Class I (Kb)‐restricted peptide epitope (called E. nanofibers) without the use of adjuvant. We demonstrate that incorporation of Tat, a cell‐penetrating peptide (CPP) of the HIV transactivator protein, into E. nanofibers remarkably enhanced tumor‐specific CD8+ T cell responses. E. nanofibers containing 12.5% Tat peptide (E.Tat12.5 nanofiber) increased antigen cross‐presentation by bone marrow‐derived dendritic cells as compared with E. nanofibers, or E. nanofibers containing 25 or 50% the Tat peptide. Uptake of KFE8.Tat12.5 nanofibers by dendritic cells (DCs) was significantly increased compared with KFE8 nanofiber lacking Tat. Peritoneal and lymph node DCs of mice immunized with E.Tat12.5 nanofibers exhibited increased presentation of the H2kb‐epitope (reminiscent for cross‐presentation) compared with DCs obtained from E. nanofiber vaccinated mice. Tetrameric and intracellular cytokine staining revealed that vaccination with E.Tat12.5 triggered a robust and specific CD8+ T lymphocyte response, which was more pronounced than in mice vaccinated with E. nanofibers alone. Furthermore, E.Tat12.5 nanofibers were more potent than E. nanofiber to induce antitumor immune response and tumor‐infiltrating IFN‐γ CD8 T lymphocyte. In terms of cancer vaccine development, we propose that harnessing the nanofiber‐based vaccine platform with incorporated Tat peptide could present a simple and promising strategy to induce highly effective antitumor immune response. 相似文献
10.
Masoodifar Mahsa Hajihashemi Saeed Pazhoohan Saeed Nazemi Samad Mojadadi Mohammad-Shafi 《Purinergic signalling》2021,17(1):143-150
Purinergic Signalling - Recent studies have shown that mesenchymal stem cells (MSCs) and their conditioned medium (CM) have potential therapeutic effects in animal models of neuropathic pain (NP).... 相似文献