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1.
The psbQ gene encoding a 16-kDa polypeptide of the oxygen-evolving complex of photosystem II has been isolated from Arabidopsis thaliana and characterized. The gene consists of a 28 nucleotide long leader sequence, two introns and three exons encoding a 223-amino-acid precursor polypeptide. The first 75 amino acids act as a transit peptide for the translocation of the polypeptide into the thylakoid lumen. Expression studies show that the gene is light-inducible and expresses only in green tissues with high steady-state mRNA levels in leaves. Using this gene as a probe, restriction fragment length polymorphism between two ecotypes, Columbia and Estland, has also been detected. 相似文献
2.
Sporotrichum thermophile Growth, Cellulose Degradation, and Cellulase Activity 总被引:3,自引:1,他引:2 下载免费PDF全文
The activity of components of the extracellular cellulase system of the thermophilic fungus Sporotrichum thermophile showed appreciable differences between strains; β-glucosidase (EC 3.2.1.21) was the most variable component. Although its endoglucanase (EC 3.2.1.4) and exoglucanase (EC 3.2.1.91) activities were markedly lower, S. thermophile degraded cellulose faster than Trichoderma reesei. The production of β-glucosidase lagged behind that of endoglucanase and exoglucanase. The latter activities were produced during active growth. When growth was inhibited by cycloheximide treatment, the hydrolysis of cellulose was lower than in the control in spite of the presence of both endoglucanase and exoglucanase activities in the culture medium. Degradation of cellulose was a growth-associated process, with cellulase preparations hydrolyzing cellulose only to a limited extent. The growth rate and cell density of S. thermophile were similar in media containing cellulose or glucose. A distinctive feature of fungal development in media incorporating cellulose or lactose (inducers of cellulase activity) was the rapid differentiation of reproductive units and autolysis of hyphal cells to liberate propagules which were capable of renewing growth immediately. 相似文献
3.
Low infectivity of vesicular stomatitis virus (VSV) particles released from interferon-treated cells is related to glycoprotein deficiency 总被引:1,自引:0,他引:1
R K Maheshwari M M Husain R M Friedman 《Biochemical and biophysical research communications》1983,117(1):161-168
We have investigated the mechanism for the low infectivity of vesicular stomatitis virus (VSV) released from interferon (IFN) -treated cells. With 10-30 units/ml of IFN there was an approximately 5-30 fold reduction in the production of virus particles, as measured by VSV proteins; however, the infectivity of the VSV released from IFN-treated mouse LB, JLS-V9R, or human GM2504 was drastically reduced (2 to 4 logs). The low infectivity of VSV was directly related to a deficiency in virion glycoprotein (G). IFN treatment did not change the specific infectivity of the VSV particles released by HeLa cells; their G protein was also not reduced. A further effect of IFN to reduce the amount of virion M protein appeared to be secondary and was probably not related to the reduced infectivity of VSV. 相似文献
4.
Flowering in Wolffia microscopica, a short-day plant, couldbe induced with salicylic acid (SA), under long days. Aspirin,benzoic acid and salicylaldoxime were also effective for inductionof flowering in this duckweed. Amonsgt these, SA is the mosteffective compound, as it could induce flowering even at 107M. Flowering was further enhanced when Wolffia fronds were subjectedto short days, in the presence of SA. However, SA neither showedany effect on flowering ofW. microscopica in the absence ofEDTA in the nutrient medium, nor could it, by itself, supporteven the vegetative growth. The probable mechanism of actionof SA has also been discussed. It appears that the effect cannotbe due simply to chelation of metal ions and perhaps the salicylmoiety itself exerts a specific effect. (Received March 15, 1983; Accepted May 6, 1983) 相似文献
5.
Maheshwari, Satish C., and R. N. Kapil. (U. Delhi, Delhi, India.) Morphological and embryological studies on the Lemnaceae. I. The floral structure and gametophytes of Lemna paucicostata. Amer. Jour. Bot. 50(7): 677–686. Illus. 1963.—In Lemna paucicostata, a locally occurring member of the Lemnaceae, the plant body is represented by a frond which is devoid of lignified elements. The root shows a winged root sheath but does not have root hairs. There are no distinctive layers like the endodermis and xylem. The male archesporium is hypodermal and differentiates normally as in other angiosperms into parietal and sporogenous layers. The tapetum is single-layered and plasmodial. The partition walls of the anther are not derived by sterilization of the sporogenous cells as believed earlier. The microspore tetrads are isobilateral and decussate, the meiotic divisions being successive. The pollen grains are shed at the 3-celled stage. The ovary contains a single hemianatropous, bitegminal and crassinucellar ovule. The development of the embryo sac conforms to the Allium type. 相似文献
6.
The present investigation dealt with the effect of the two granular systemic nematicides aldicarb and carbofuran, individually and in combination with 100 mg · 1-1 GA3, on plant growth and root knot formation in C. frutescens var. California Wonder. Both nematicides and GA3 were applied as a seed-dip treatment. GA3 increased seed germination and seedling growth. All concentrations (2 to 20 g a.i. · 1-1) of aldicarb and carbofuran, when applied independently, reduced seed germination and length of epicotyl and hypocotyl. However, application of these nematicides in combination with 100 mg · 1-1 GA3 showed antagonistic behaviour. All the treated seedlings were inoculated with 1000 nematode larvae of Meloidogyne incognita. Vegetative parameters of the plant were studied 15 days after transplantation. A combination of GA3 (100 mg · 1-1) with different concentrations of nematicides showed a synergistic effect on treated plants. Root knot index in plants treated with nematicides was found to be reduced with increasing concentrations of both nematicides. Maximum reduction in root knot index was observed at highest dose (20 g a.i. · 1-1) of both nematicides, when applied in combination with 100 mg · 1-1 GA3. Proteolytic activities were decreased in plants treated with both aldicarb and carbofuran. Supplementation of the nematicides with GA3 resulted in an increase in enzyme activities of the treated plants. Toxic residues increased with increasing concentration of both nematicides, whereas it was reduced at highest concentration of both nematicides when applied in combination with GA3. 相似文献
7.
The life-history ofNeurospora in nature has remained largely unknown. The present study attempts to remedy this. The following conclusions are based on
observation ofNeurospora on fire-scorched sugar cane in agricultural fields, and reconstruction experiments using a colour mutant to inoculate sugar
cane burned in the laboratory. The fungus persists in soil as heat- resistant dormant ascospores. These are activated by a
chemical(s) released into soil from the burnt substrate. The chief diffusible activator of ascospores is furfural and the
germinating ascospores infect the scorched substrate. An invasive mycelium grows progressively upwards inside the juicy sugar
cane and produces copious macroconidia externally through fire- induced openings formed in the plant tissue, or by the mechanical
rupturing of the plant epidermal tissue by the mass of mycelium. The loose conidia are dispersed by wind and/or foraged by
microfauna. It is suggested that the constant production of macroconidia, and their ready dispersal, serve a physiological
role: to drain the substrate of minerals and soluble sugars, thereby creating nutritional conditions which stimulate sexual
reproduction by the fungus. Sexual reproduction in the sugar- depleted cellulosic substrate occurs after macroconidiation
has ceased totally and is favoured by the humid conditions prevailing during the monsoon rains. Profuse micro-conidiophores
and protoperithecia are produced simultaneously in the pockets below the loosened epidermal tissue. Presumably protoperithecia
are fertilized by microconidia which are possibly transmitted by nematodes active in the dead plant tissue. Mature perithecia
release ascospores in situ which are passively liberated in the soil by the disintegration of the plant material and are,
apparently, distributed by rain or irrigation water. 相似文献
8.
Suresh Chand Purnima Basu Neeti Maheshwari 《Journal of plant biochemistry and biotechnology.》1994,3(1):53-54
Somatic embryogenesis and regeneration of plantlets was achieved In callus cultures derived from cotyledonary leaf pieces of Hyoscyamus muticus L on MS medium enriched with 2 mg/l 2,4–0 and 0.5 mg/l BAP. For embryogenesis and organogenesis varying concentrations of NAA with or without BAP were added In the medium. Organogenesis was also achieved when callus was transferred to the hormone free medium. 相似文献
9.
Akalla Mahalakshmi Satish C. Maheshwari Paramjit Khurana 《Journal of plant biochemistry and biotechnology.》1993,2(1):61-65
Protoplasts were isolated from the basal meristematic region of leaves from 6-day-old seedlings of wheat (Triticum aestivum). Protoplasts divided when cultured on MS medium (as agarose beads) in presence of nurse tissue. Donor seedlings when grown on BAP-supplemented MS medium were found to be considerably superior for protoplast isolation and culture than when grown on MS basal medium, in terms of protoplast viability, cell wall formation and cell division frequency. In addition, reduction of ammonium content of the culture medium, together with a dark Incubation, led to a high protoplast division frequency of about 70%. Microcolonies of 10-to 12-celled stages were obtained in Triticum aestivum, varieties HD 2329, HD 2285, Kalyan Sona, Arjun and CPAN 1676. 相似文献
10.
Two new pregnane ester glycosides designated as pallidine and pallidinine have been isolated from the dried twigs of Pergularia pallida. Chemical and spectroscopic evidences are consistent with the structure 12,20-di-O-benzoyl sarcostin-3-O-β-d-oleandroside and 12,20-di-O-benzoyl-sarcostin-3-O-β-d-cymaropyranosyl(1 → 4)-β-d-oleandropyranoside for pallidine and pallidinine respectively. 相似文献