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Ben Salem Nesrine Boussetta Sami de Rojas Itziar Moreno-Grau Sonia Montrreal Laura Mokni Narjes Mahmoud Imene Younes Samia Daouassi Nizar Frih-Ayed Mahbouba Hammami Afef Ben Ammar Elgaaied Amel Ruiz Agustín Cherni Lotfi 《Molecular biology reports》2022,49(3):1687-1700
Molecular Biology Reports - Alzheimer’s disease (AD) is the most common neurodegenerative disorder in humans and presents a major health problem throughout the world. The etiology of AD is... 相似文献
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An electron paramagnetic resonance study of native and modified freeze-dried cupric insulin hexamer.
Cupric insulin was modified by the addition of cross-linking disulphide bridges between hexamers. The electron paramagnetic resonance (EPR) spectrum of this freeze-dried material was compared with that of freeze-dried unmodified cupric insulin containing various amounts of copper and added water. The modified insulin was found to have cupric ion sites magnetically very similar to that of native insulin containing two cupric ions per hexamer. Native hexamer produced in the presence of 2 Cu(II) ions per hexamer gave, after freeze-drying, an EPR spectrum with A∥Cu=16.5 mT, g∥=2.285 and g⊥=2.059 (site 1). The use of 4 or 6 Cu(II) ions per hexamer resulted in spectra with two components-a major component with the same A∥Cu and g∥ values as the sample containing 2 Cu(II) ions (site 1) and an additional minor component (site 2). These sites have been identified with the analogous zinc binding site within the hexamer formed by three B-10 histidine residues (site 1) [1, 2] and the site formed by the B-1 α-amino and A-17 glutamyl-γ-barboxylic acid functions where excess zinc is bound (site 2) [3, 4]. The addition of water to native hexamer containing 2, 4, or 6 Cu(II) ions resulted in the appearance of three distinct EPR absorptions, one of which had the same parameters as the freeze-dried native insulin containing 2 Cu(II) ions per hexamer (site 1). Two further sites appeared (3 and 4) with the following parameters: A∥Cu=15.0 mT, g∥=2.353, and g⊥=2.07; A∥Cu=16.5 mT, g∥=2.315, and g⊥=2.07, respectively. 相似文献
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