全文获取类型
收费全文 | 574篇 |
免费 | 48篇 |
出版年
2023年 | 4篇 |
2022年 | 10篇 |
2021年 | 20篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 8篇 |
2017年 | 13篇 |
2016年 | 18篇 |
2015年 | 36篇 |
2014年 | 30篇 |
2013年 | 34篇 |
2012年 | 71篇 |
2011年 | 40篇 |
2010年 | 32篇 |
2009年 | 32篇 |
2008年 | 47篇 |
2007年 | 27篇 |
2006年 | 39篇 |
2005年 | 29篇 |
2004年 | 34篇 |
2003年 | 27篇 |
2002年 | 27篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1976年 | 1篇 |
1973年 | 1篇 |
1913年 | 1篇 |
1901年 | 1篇 |
排序方式: 共有622条查询结果,搜索用时 15 毫秒
1.
2.
3.
Jayde A. Gawthorne L. Evan Reddick Snezhana N. Akpunarlieva Katherine S. H. Beckham John M. Christie Neal M. Alto Mads Gabrielsen Andrew J. Roe 《PloS one》2012,7(12)
In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function. Specifically, we used iLOV, an enhanced and more photostable variant of LOV. A pET-based plasmid for protein expression was constructed, encoding a C terminal iLOV-octahistidine (His8)-tag and a HRV 3C protease cleavage recognition site. Ten different proteins, with various sub-cellular locations, were cloned into the plasmid, creating iLOV-His8 tag fusions. To test protein expression and how iLOV could be used as a reporter, the proteins were expressed in three different cell lines, in four different culture media, at two different temperatures. To establish whether the presence of the iLOV tag could have an impact on the functionality, one of the proteins, EspG, was over-expressed and purified. EspG is an “effector” protein normally produced by enterohemorrhagic E. coli strains and “injected” into host cells via the T3SS. We tested functionality of EspG-iLOV fusion by performing functional studies of EspG in mammalian host cells. When EspG-iLOV was microinjected into the host cell, the Golgi apparatus was completely disrupted as had previously been observed for EspG. 相似文献
4.
5.
Katrine A. Gorham Mads P. Sulbaek Andersen Simone Meinardi Ralph J. Delfino Norbert Staimer Thomas Tjoa 《Biomarkers》2013,18(1):17-25
The relationship of exhaled ethane and n-pentane to exhaled NO, carbonylated proteins, and indoor/outdoor atmospheric pollutants were examined in order to evaluate ethane and n-pentane as potential markers of airway inflammation and/or oxidative stress. Exhaled NO and carbonylated proteins were found to have no significant associations with either ethane (p = 0.96 and p = 0.81, respectively) or n-pentane (p = 0.44 and 0.28, respectively) when outliers were included. In the case where outliers were removed n-pentane was found to be inversely associated with carbonylated proteins. Exhaled hydrocarbons adjusted for indoor hydrocarbon concentrations were instead found to be positively associated with air pollutants (NO, NO2 and CO), suggesting pollutant exposure is driving exhaled hydrocarbon concentrations. Given these findings, ethane and n-pentane do not appear to be markers of airway inflammation or oxidative stress. 相似文献
6.
7.
Leslie K. Ferrarelli Venkateswarlu Popuri Avik K. Ghosh Takashi Tadokoro Chandrika Canugovi Joseph K. Hsu Deborah L. Croteau Vilhelm A. Bohr 《DNA Repair》2013,12(7):518-528
Telomeres are critical for cell survival and functional integrity. Oxidative DNA damage induces telomeric instability and cellular senescence that are associated with normal aging and segmental premature aging disorders such as Werner Syndrome and Rothmund–Thomson Syndrome, caused by mutations in WRN and RECQL4 helicases respectively. Characterizing the metabolic roles of RECQL4 and WRN in telomere maintenance is crucial in understanding the pathogenesis of their associated disorders. We have previously shown that WRN and RECQL4 display a preference in vitro to unwind telomeric DNA substrates containing the oxidative lesion 8-oxoguanine. Here, we show that RECQL4 helicase has a preferential activity in vitro on telomeric substrates containing thymine glycol, a critical lesion that blocks DNA metabolism, and can be modestly stimulated further on a D-loop structure by TRF2, a telomeric shelterin protein. Unlike that reported for telomeric D-loops containing 8-oxoguanine, RECQL4 does not cooperate with WRN to unwind telomeric D-loops with thymine glycol, suggesting RECQL4 helicase is selective for the type of oxidative lesion. RECQL4's function at the telomere is not yet understood, and our findings suggest a novel role for RECQL4 in the repair of thymine glycol lesions to promote efficient telomeric maintenance. 相似文献
8.
Simon Glerup Maria Lume Ditte Olsen Jens R. Nyengaard Christian B. Vaegter Camilla Gustafsen Erik I. Christensen Mads Kjolby Anders Hay-Schmidt Dirk Bender Peder Madsen Mart Saarma Anders Nykjaer Claus M. Petersen 《Cell reports》2013,3(1):186-199
- Download : Download full-size image
9.
Basu U Gyrd-Hansen M Baby SM Lozynska O Krag TO Jensen CJ Frödin M Khurana TS 《FEBS letters》2007,581(22):4153-4158
Utrophin is the autosomal homolog of dystrophin, the product of the Duchenne's muscular dystrophy (DMD) locus. Utrophin is of therapeutic interest since its over-expression can compensate dystrophin's absence. Utrophin is enriched at neuromuscular junctions due to heregulin-mediated utrophin-A promoter activation. We demonstrate that heregulin activated MSK1/2 and phosphorylated histone H3 at serine 10 in cultured C2C12 muscle cells, in an ERK-dependent manner. MSK1/2 inhibition suppressed heregulin-mediated utrophin-A activation. MSK1 over-expression potentiated heregulin-mediated utrophin-A activation and chromatin remodeling at the utrophin-A promoter. These results identify MSK1/2 as key effectors modulating utrophin-A expression as well as identify novel targets for DMD therapy. 相似文献
10.
Burak Avc Jakob Brandt Dikla Nachmias Natalie Elia Mads Albertsen Thijs J. G. Ettema Andreas Schramm Kasper Urup Kjeldsen 《The ISME journal》2022,16(2):606
The origin of the eukaryotic cell is a major open question in biology. Asgard archaea are the closest known prokaryotic relatives of eukaryotes, and their genomes encode various eukaryotic signature proteins, indicating some elements of cellular complexity prior to the emergence of the first eukaryotic cell. Yet, microscopic evidence to demonstrate the cellular structure of uncultivated Asgard archaea in the environment is thus far lacking. We used primer-free sequencing to retrieve 715 almost full-length Loki- and Heimdallarchaeota 16S rRNA sequences and designed novel oligonucleotide probes to visualize their cells in marine sediments (Aarhus Bay, Denmark) using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Super-resolution microscopy revealed 1–2 µm large, coccoid cells, sometimes occurring as aggregates. Remarkably, the DNA staining was spatially separated from ribosome-originated FISH signals by 50–280 nm. This suggests that the genomic material is condensed and spatially distinct in a particular location and could indicate compartmentalization or membrane invagination in Asgard archaeal cells.Subject terms: Soil microbiology, Microbial ecology, Archaeal physiology 相似文献