全文获取类型
收费全文 | 22194篇 |
免费 | 1911篇 |
国内免费 | 14篇 |
出版年
2012年 | 2348篇 |
2011年 | 2725篇 |
2010年 | 394篇 |
2009年 | 190篇 |
2008年 | 2129篇 |
2007年 | 2144篇 |
2006年 | 2083篇 |
2005年 | 1945篇 |
2004年 | 1808篇 |
2003年 | 1822篇 |
2002年 | 1508篇 |
2001年 | 1216篇 |
2000年 | 1705篇 |
1999年 | 657篇 |
1998年 | 86篇 |
1997年 | 56篇 |
1996年 | 56篇 |
1995年 | 56篇 |
1994年 | 32篇 |
1993年 | 26篇 |
1992年 | 40篇 |
1991年 | 34篇 |
1990年 | 29篇 |
1989年 | 32篇 |
1988年 | 27篇 |
1987年 | 41篇 |
1986年 | 26篇 |
1985年 | 14篇 |
1984年 | 37篇 |
1983年 | 28篇 |
1982年 | 17篇 |
1981年 | 14篇 |
1980年 | 17篇 |
1978年 | 15篇 |
1975年 | 14篇 |
1974年 | 16篇 |
1970年 | 18篇 |
1968年 | 13篇 |
1959年 | 33篇 |
1958年 | 65篇 |
1957年 | 70篇 |
1956年 | 60篇 |
1955年 | 51篇 |
1954年 | 47篇 |
1953年 | 42篇 |
1952年 | 49篇 |
1951年 | 25篇 |
1950年 | 36篇 |
1949年 | 37篇 |
1948年 | 30篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
Genome-wide analysis of the SET DOMAIN GROUP family in grapevine 总被引:1,自引:0,他引:1
The SET DOMAIN GROUP (SDG) proteins represent an evolutionarily-conserved family of epigenetic regulators present in eukaryotes
and are putative candidates for the catalysis of lysine methylation in histones. Plant genomes analyses of this family have
been performed in arabidopsis, maize, and rice and functional studies have shown that SDG genes are involved in the control
of plant development. In this work, we describe the identification and structural characterization of SDG genes in the Vitis vinifera genome. This analysis revealed the presence of 33 putative SDG genes that can be grouped into different classes, as it has
been previously described for plants. In addition to the SET domain, the proteins identified possessed other domains in the
different classes. As part of our study regarding the growth and development of grapevine, we selected eight genes and their
expression levels were analyzed in representative vegetative and reproductive organs of this species. The selected genes showed
different patterns of expression during inflorescence and fruit development, suggesting that they participate in these processes.
Furthermore, we showed that the expression of selected SDGs changes during viral infection, using as a model Grapevine Leafroll
Associated Virus 3-infected symptomatic grapevine leaves and fruits. Our results suggest that developmental changes caused
by this virus could be the result of alterations in SDG expression. 相似文献
4.
5.
6.
7.
8.
The membranes of lipid bodies from the endosperm of seeds of Ricinus communis have long been known to contain an acid lipase (triacylglycerol acyl hydrolase, EC 3.1.1.3). The means by which fat hydrolysis is initiated and controlled in the endosperm of the young seedling are not yet understood, although it is generally assumed that the acid lipase is the enzyme responsible for the conversion of stored triacylglycerols to fatty acids and glycerol. However, the enzyme from seeds is not an effective catalyst at cytoplasmic pH since it has a pH optimum at 4.5 and is virtually inactive above pH 6.0. The results described in this paper show that during early growth of castor seeds the lipid bodies acquire a lipase which is active at neutral pH values. The lipase is absent from dry seeds, appears at day 3, and increases rapidly in activity until day 5. The pattern of appearance of the lipase mirrors that of other enzymes involved in the conversion of fat to sugar. The lipase is stimulated 40-fold by 30 micromolar free Ca2+ and the activity at pH 7.0 to 7.5 adequately accounts for the known rate of triacylglycerol hydrolysis in vivo. 相似文献
9.
A calcium-dependent protein kinase activity from suspension-cultured soybean cells (Glycine max L. Wayne) was shown to be dependent on calcium but not calmodulin. The concentrations of free calcium required for half-maximal histone H1 phosphorylation and autophosphorylation were similar (≈2 micromolar). The protein kinase activity was stimulated 100-fold by ≥10 micromolar-free calcium. When exogenous soybean or bovine brain calmodulin was added in high concentration (1 micromolar) to the purified kinase, calcium-dependent and -independent activities were weakly stimulated (≤2-fold). Bovine serum albumin had a similar effect on both activities. The kinase was separated from a small amount of contaminating calmodulin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. After renaturation the protein kinase autophosphorylated and phosphorylated histone H1 in a calcium-dependent manner. Following electroblotting onto nitrocellulose, the kinase bound 45Ca2+ in the presence of KCl and MgCl2, which indicates that the kinase itself is a high-affinity calcium-binding protein. Also, the mobility of one of two kinase bands in SDS gels was dependent on the presence of calcium. Autophosphorylation of the calmodulin-free kinase was inhibited by the calmodulin-binding compound N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (W-7), showing that the inhibition of activity by W-7 is independent of calmodulin. These results show that soybean calcium-dependent protein kinase represents a new class of protein kinase which requires calcium but not calmodulin for activity. 相似文献
10.
Subcellular localization of glycosidases and glycosyltransferases involved in the processing of N-linked oligosaccharides 总被引:10,自引:2,他引:8
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Using isopycnic sucrose gradients, we have ascertained the subcellular location of several enzymes involved in the processing of the N-linked oligosaccharides of glycoproteins in developing cotyledons of the common bean, Phaseolus vulgaris. All are localized in the endoplasmic reticulum (ER) or Golgi complex as determined by co-sedimentation with the ER marker, NADH-cytochrome c reductase, or the Golgi marker, glucan synthase I. Glucosidase activity, which removes glucose residues from Glc3Man9(GlcNAc)2, was found exclusively in the ER. All other processing enzymes, which act subsequent to the glucose trimming steps, are associated with the Golgi. These include mannosidase I (removes 1-2 mannose residues from Man6-9[GlcNAc]2), mannosidase II (removes mannose residues from GlcNAcMan5[GlcNAc]2), and fucosyltransferase (transfers a fucose residue to the Asn-linked GlcNAc of appropriate glycans). We have previously reported the localization of two other glycan modifying enzymes (GlcNAc-transferase and xylosyltransferase activities) in the Golgi complex. Attempts at subfractionation of the Golgi fraction on shallow sucrose gradients yielded similar patterns of distribution for all the Golgi processing enzymes. Subfractionation on Percoll gradients resulted in two peaks of the Golgi marker enzyme inosine diphosphatase, whereas the glycan processing enzymes were all enriched in the peak of lower density. These results do not lend support to the hypothesis that N-linked oligosaccharide processing enzymes are associated with Golgi cisternae of different densities. 相似文献