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C. L. REARDON A. C. DOHNALKOVA P. NACHIMUTHU D. W. KENNEDY D. A. SAFFARINI B. W. AREY L. SHI Z. WANG D. MOORE J. S. MCLEAN D. MOYLES M. J. MARSHALL J. M. ZACHARA J. K. FREDRICKSON A. S. BELIAEV 《Geobiology》2010,8(1):56-68
In an effort to improve the understanding of electron transfer mechanisms at the microbe–mineral interface, Shewanella oneidensis MR-1 mutants with in-frame deletions of outer-membrane cytochromes (OMCs), MtrC and OmcA, were characterized for the ability to reduce ferrihydrite (FH) using a suite of microscopic, spectroscopic, and biochemical techniques. Analysis of purified recombinant proteins demonstrated that both cytochromes undergo rapid electron exchange with FH in vitro with MtrC displaying faster transfer rates than OmcA. Immunomicroscopy with cytochrome-specific antibodies revealed that MtrC co-localizes with iron solids on the cell surface while OmcA exhibits a more diffuse distribution over the cell surface. After 3-day incubation of MR-1 with FH, pronounced reductive transformation mineral products were visible by electron microscopy. Upon further incubation, the predominant phases identified were ferrous phosphates including vivianite [Fe3 (PO4 )2 ·8H2 O] and a switzerite-like phase [Mn3 ,Fe3 (PO4 )2 ·7H2 O] that were heavily colonized by MR-1 cells with surface-exposed outer-membrane cytochromes. In the absence of both MtrC and OmcA, the cells ability to reduce FH was significantly hindered and no mineral transformation products were detected. Collectively, these results highlight the importance of the outer-membrane cytochromes in the reductive transformation of FH and support a role for direct electron transfer from the OMCs at the cell surface to the mineral. 相似文献
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Effects of ultraviolet light on poliomyelitis virus 总被引:3,自引:0,他引:3
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The nature of the formalin inactivation of poliomyelitis virus 总被引:2,自引:0,他引:2
HOOK AE KUPSKY CH MCLEAN IW TIMM EA 《Journal of immunology (Baltimore, Md. : 1950)》1956,77(6):444-452
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Evidence for mate‐encounter Allee effect in an invasive longhorn beetle (Coleoptera: Cerambycidae)
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MARC RHAINDS STEPHEN B. HEARD CORY HUGHES WAYNE MACKINNON KEVIN PORTER JON SWEENEY PETER SILK IAN DEMERCHANT SARAH MCLEAN GARRETT BRODERSEN 《Ecological Entomology》2015,40(6):829-832
1. Limited empirical support is available for mate‐encounter Allee effects in invasive insects due to the logistical challenges of studying demographic trends in low‐density populations. 2. Traps baited with pheromone and spruce volatiles were used to monitor the abundance of female Tetropium fuscum F. (Coleoptera: Cerambycidae) at multiple sites in Nova Scotia in 2011 and 2012. Each female was dissected to determine the presence or absence of sperm in its spermatheca (mated or virgin female, respectively). 3. Both male and total T. fuscum abundance declined with increasing distance to the focal point of T. fuscum's invasion. Female mating probability declined with male abundance and with distance from the invasion focus, and mating probabilities were very low at the most peripheral sites. Difficulty in encountering mates may thus contribute to limiting the spread of T. fuscum. 4. The approach outlined here could be integrated into existing surveys of wood borers using traps baited with semiochemicals to improve our understanding of the role of the mate‐encounter Allee effect in invasion dynamics. 相似文献
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