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JANINA KACZANOWSKA LEONORA BUZANSKA MAREK OSTROWSKI 《The Journal of eukaryotic microbiology》1993,40(6):747-754
Microtubular basal bodies and epiplasm (membrane skeleton) are the main components of the cortical skeleton of Tetrahymena. The aim of this report was to study functional interactions of basal bodies and epiplasm during the cell cycle. The cortex of Tetrahymena cells was stained with anti-epiplasm antibody. This staining produced a bright epiplasmic layer with a dark pattern of unstained microtubular structures. The fluorescence of the anti-epiplasm antibody disappeared at sites of newly formed microtubular structures, so the new basal body domains and epiplasmic layer could be followed throughout the cell cycle. Different patterns of deployment of new basal bodies were observed in early and advanced dividers. In advanced dividers the fluorescence of the epiplasmic layer diminished locally within the forming fission line where the polymerization of new basal bodies largely extincted. In wild type Tetrahymena, the completion of the micronuclear metaphase/anaphase transition was associated with a transition from the pattern of new basal body deployment and epiplasm staining of the early divider to the pattern of the advanced dividers. The signal for the fission line formation in Tetrahymena (absent in cdaA1 Tetrahymena mutationally arrested in cytokinesis) brings about 1) transition of patterns of deployment of basal bodies and epiplasmic layer on both sides of the fission line; and 2) coordination of cortical divisional morphogenesis with the micronuclear mitotic cycle. 相似文献
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MARLENE BENCHIMOL CEZAR ANTONIO ELIAS WANDERLEY DE SOUZA 《The Journal of eukaryotic microbiology》1982,29(3):348-353
ABSTRACT. Freeze-fracture techniques reveal differences in fine structure between the anterior three flagella of Tritrichomonas foetus and its recurrent flagellum. The anterior flagella have rosettes of 9–12 intramembranous particles on both the P and E faces. The recurrent flagellum lacks rosettes but has ribbon-like arrays of particles along the length of the flagellum, which may be involved in the flagellum's attachment to the cell body. This flagellum is attached to the membrane of the cell body along a distinct groove that contains few discernible particles. Some large intramembranous particles are visible on the P face of the cell body membrane at the point where the flagellum emerges from the cell body. The randomly distributed particles on the P and E faces of the plasma membrane have a particle density of 919/μm2 and 468/μm2 respectively, and there are areas on both faces that are devoid of particles. Freeze-fracture techniques also reveal numerous fenestrations in the membrane of the Golgi complex and about 24 pores per μm2 in the nuclear. membrane. 相似文献
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CYRIL BONTEMPS GEOFFREY N. ELLIOTT† MARCELO F. SIMON‡ FÁBIO B. DOS REIS JÚNIOR § EDUARDO GROSS¶ REBECCA C. LAWTON NICOLAU ELIAS NETO MARIA de FÁTIMA LOUREIRO SERGIO M. DE FARIA†† JANET I. SPRENT‡‡ EUAN K. JAMES§§ J. PETER W. YOUNG 《Molecular ecology》2010,19(1):44-52
Burkholderia has only recently been recognized as a potential nitrogen-fixing symbiont of legumes, but we find that the origins of symbiosis in Burkholderia are much deeper than previously suspected. We sampled 143 symbionts from 47 native species of Mimosa across 1800 km in central Brazil and found that 98% were Burkholderia . Gene sequences defined seven distinct and divergent species complexes within the genus Burkholderia . The symbiosis-related genes formed deep Burkholderia -specific clades, each specific to a species complex, implying that these genes diverged over a long period within Burkholderia without substantial horizontal gene transfer between species complexes. 相似文献
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LADISLAV MAREK ELLIS L. YOCHELSON 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1976,9(1):65-82
Hyolitha constitute an extinct group of class rank assigned to Molhsca. Two orders, Ortho-thecida and Hyolithida, are well established and most knowledge of morphologic detail is derived from the latter. Both orders had an operculum not hinged to the conch. Hyolithida also had paired, curved, whiskerlike appendages, requiring complex musculature to move them and the operculum. The Hyolithida were probably deposit feeders living in shallow water, and accordingly were tentaculate. A reconstruction of the soft parts of this sedentary organism is given — a shallow mantle cavity on the dorsal side, anterior tentacles, a long intestine, and a reduced ventral foot. Except for the more complex musculature associated with an elaborate operculum, Orthothecida are judged to have had a similar anatomy. 相似文献
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TEMPERATURE AND THE GROWTH OF IMPATIENS PARVIFLORA DC. 总被引:2,自引:2,他引:0