全文获取类型
收费全文 | 594篇 |
免费 | 44篇 |
出版年
2022年 | 2篇 |
2021年 | 12篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 11篇 |
2016年 | 6篇 |
2015年 | 22篇 |
2014年 | 24篇 |
2013年 | 27篇 |
2012年 | 49篇 |
2011年 | 34篇 |
2010年 | 23篇 |
2009年 | 20篇 |
2008年 | 34篇 |
2007年 | 33篇 |
2006年 | 28篇 |
2005年 | 34篇 |
2004年 | 37篇 |
2003年 | 24篇 |
2002年 | 24篇 |
2001年 | 15篇 |
2000年 | 11篇 |
1999年 | 9篇 |
1998年 | 10篇 |
1997年 | 8篇 |
1996年 | 3篇 |
1995年 | 5篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 11篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 9篇 |
1988年 | 3篇 |
1987年 | 12篇 |
1986年 | 7篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1977年 | 3篇 |
1975年 | 3篇 |
1969年 | 2篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1963年 | 1篇 |
1959年 | 1篇 |
排序方式: 共有638条查询结果,搜索用时 46 毫秒
1.
Rosette and single-element strain gauges were implanted on the tibia in 2 dogs and recordings were made during locomotion on a treadmill. At foot contact and during the swing phase of locomotion, bone strains were low and directions of the principal strains were variable. There was a large shift in the directions of the principal strains at the beginning of the stance phase and bone strains were considerably higher. Peak strain occurred midway through the stance phase. At that time, the maximum principal strain (tension) was directed upwards and anteriorly between 30 and 60 degrees with respect to the long axis of the tibia. These bone strain patterns in the dog are similar to those found in sheep while both differ markedly from those found in humans. 相似文献
2.
Marianne Bouvier 《American journal of primatology》1988,15(2):129-142
A new technique has been developed for estimating age in rhesus macaques (Macaca mulatta) that is based on mandibular measurements and has several advantages over presently available techniques. First, the new technique is relatively quick and inexpensive because a single measurement or radiograph suffices to produce an age estimate. Second, mandibular growth represents a more continuous phenomenon than either epiphyseal union or dental eruption; therefore, age estimates may be made throughout the period of growth without significant gaps in the process. Finally, the new technique may be used on incomplete or even fragmentary skeletal material because it requires that only a portion of the mandible be preserved. The new technique produces age estimates with a prediction error of ±5.08 months in males and ±7.29 months in females. These errors are only slightly higher than those found previously for dental eruption or epiphyseal union data. 相似文献
3.
4.
5.
Lydie M. Dupont Hans Peter Linder Florian Rommerskirchen Enno Schefuß 《Journal of Biogeography》2011,38(6):1059-1068
Aim To test whether the radiation of the extremely rich Cape flora is correlated with marine‐driven climate change. Location Middle to Late Miocene in the south‐east Atlantic and the Benguela Upwelling System (BUS) off the west coast of South Africa. Methods We studied the palynology of the thoroughly dated Middle to Late Miocene sediments of Ocean Drilling Program (ODP) Site 1085 retrieved from the Atlantic off the mouth of the Orange River. Both marine upwelling and terrestrial input are recorded at this site, which allows a direct correlation between changes in the terrestrial flora and the marine BUS in the south‐east Atlantic. Results Pollen types from plants of tropical affinity disappeared, and those from the Cape flora gradually increased, between 10 and 6 Ma. Our data corroborate the inferred dating of the diversification in Aizoaceae c. 8 Ma. Main conclusions Inferred vegetation changes for the Late Miocene south‐western African coast are the disappearance of Podocarpus‐dominated Afromontane forests, and a change in the vegetation of the coastal plain from tropical grassland and thicket to semi‐arid succulent vegetation. These changes are indicative of an increased summer drought, and are in step with the development of the southern BUS. They pre‐date the Pliocene uplift of the East African escarpment, suggesting that this did not play a role in stimulating vegetation change. Some Fynbos elements were present throughout the recorded period (from 11 Ma), suggesting that at least some elements of this vegetation were already in place during the onset of the BUS. This is consistent with a marine‐driven climate change in south‐western Africa triggering substantial radiation in the terrestrial flora, especially in the Aizoaceae. 相似文献
6.
Florence Levy-Favatier Lydie Tichnonicky Jacques Kruh Marc Delpech 《Biochimie》1989,71(11-12):1157-1161
We have previously described in rat liver two protein kinases tightly bound to DNA, one is serine-specific, the other arginine-specific. In this work we show that both enzymes are present in various rat tissues and in liver from various species. Both kinase specific activities are strongly decreased in methyl-DBA-induced hepatomas and in HTC cells but not in regenerating liver after hepatectomy. This decrease is then not related to cell proliferation. 相似文献
7.
Using plasma catecholamine (CA) levels as an index of the sympathoadrenal activity, the effects of chronic and acute beta-blockade on the blood pressure and sympathetic activity were evaluated in deoxycorticosterone acetate (DOCA) - salt hypertensive (HT) rats. The acute administration of one beta-blocker (sotalol, 5 mg/kg) to intact of vagotomized anesthetized HT animals induced a significant decrease in plasma norepinephrine (NE) concentrations and mean arterial pressure (MAP). The amplitude of the decrease of the MAP or NE levels were linearly correlated with the basal NE levels, suggesting that sotalol reduced the blood pressure and sympathetic NE release more efficiently in rats with increased sympathetic activity. Similarly, chronic infusion of sotalol (1.5 mg X day-1 X rat-1) through an osmotic pump for 12 days in DOCA-salt HT rats significantly reduced NE and epinephrine (E) plasma levels compared with those observed in untreated DOCA-salt HT rats. Moreover, the chronic treatment with sotalol significantly reduced the plasma E elevation induced by bilateral carotid occlusion (CO) in vagotomized normotensive (NT) and HT rats. It therefore appears that acute administration of sotalol to HT rats causes a significant reduction in the sympathetic activity which is associated to a decrease in MAP. Although chronic sotalol treatment causes a significant reduction in the sympathoadrenal basal activity and in the adrenal reactivity, this treatment did not prevent the development of DOCA-salt hypertension. 相似文献
8.
The major surface protein of Leishmania promastigotes is a protease 总被引:10,自引:0,他引:10
The major surface protein of Leishmania promastigotes is evolutionarily conserved and is found in isolates of L. donovani, L. major, L. tropica, L. mexicana, and L. braziliensis. The data provided in this communication demonstrate that in L. major this integral membrane protein is a protease, which we now designate promastigote surface protease. The enzyme has an alkaline pH optimum and is active both in its detergent-solubilized form and at the surface of living or fixed promastigotes. A water-soluble form of promastigote surface protease is obtained following digestion with the phospholipase C responsible for the release of the variant surface glycoprotein of Trypanosoma brucei. Possible biological functions of promastigote surface protease during the life cycle of Leishmania parasites are discussed. 相似文献
9.
Solubilization and Characterization of D2 -Dopamine Receptors in an Estrone-Induced, Prolactin-Secreting Rat Pituitary Adenoma 总被引:3,自引:2,他引:1
C. Bouvier M. Potier G. Beauregard J. Lafond N. Amlaiky† M. G. Caron† R. Collu 《Journal of neurochemistry》1986,47(5):1653-1660
D2-dopamine (3,4-dihydroxyphenylethylamine) receptors were successfully solubilized with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate from an estrone-induced rat pituitary adenoma. Forty-five percent of initial protein and 48% of initial [3H]spiroperidol binding sites were solubilized. The high affinity as well as the stereoselectivity of the sites was preserved. The order of potency of dopaminergic agonists was found to be typical of D2 receptors. Target size analysis by radiation inactivation indicated a molecular weight of 143,000 +/- 3,000 and of 106,000 +/- 4,000 daltons for membrane-bound and solubilized receptors, respectively. This suggests the loss of a 37,000-dalton subunit during solubilization without significant modification of binding characteristics. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of receptor protein preparation photolabeled with N-(p-azido-m[125I]iodophenethyl)spiroperidol confirmed the existence of a 94,000-dalton peptide which probably constitutes the ligand binding site of the receptor. Thus, our data indicate that chronic estrogen treatment of rats, although inducing a pituitary adenoma, does not modify the pharmacological characteristics of D2 receptors. These data suggest therefore that these adenoma may represent an ideal source of material for further biochemical characterization of D2 receptors. 相似文献
10.
Detection of sugar-binding proteins in membrane-depleted nuclei 总被引:1,自引:0,他引:1
A. P. Seve J. Hubert D. Bouvier M. Bouteille C. Maintier M. Monsigny 《Experimental cell research》1985,157(2):533-538
Nuclear sugar-binding proteins were detected in membrane-depleted nuclei isolated from hamster BHK cells and mouse L 1210 leukemia cells by means of fluorescein-labelled neoglycoproteins. In fluorescence microscopy, the fluorescence was seen throughout the nucleus but was generally brighter over the nucleoli than over the rest of the nucleus. Flow cytofluorometry analysis demonstrated the presence of nuclear sugar-binding proteins for synthetic glycoproteins associated with different sugar residues. Among the nine neoglycoproteins used, four neoglycoproteins (namely alpha-rhamnosylated, alpha-glucosylated, N-acetyl-beta-glucosaminylated and alpha-mannosylated-6P-serum albumin) strongly labelled nuclei. Various controls strongly argue for the specificity of the nuclear labelling. The possibility that some of the sugar-binding proteins might correspond to endogenous nuclear lectins is considered. 相似文献