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A colony of the Edible-nest Swiftlet Aerodramus fuciphagus nesting in a Chinese shophouse in Penang, Peninsular Malaysia, was studied for seven months. Birds bred throughout, but laying was concentrated in the period October to February. Incubation and fledging periods were 23 ± 3 days and 43 + 6 days, respectively. Most young hatched during the dry season. The normal clutch size was two, with many birds laying second clutches (75%) and some third clutches (15-4%) using the same nest. Clutch size showed no variation with clutch order or month. The overall hatching success was 69-0 %, most losses caused by eggs falling from or with the nest. Fledging success was similar for broods of two (625%) and broods of one (59-4%), so that the former were twice as productive. The main loss of chicks occurred when they fell from the nest and were eaten by mammalian predators. There was a tendency for breeding success to decline with successive clutches, but not with month, being highest in November (53-6 %) and February (55-6 %). The growth rate of single chicks and successful broods of two was similar, except that the second chick was more prone to fluctuations in weight. Some second chicks showed evidence of starvation before falling from the nest. Diet was examined by analysis of foodballs regurgitated by mist-netted adults. Foodballs weighed 0–13-1 08 g and, on average, contained over 500 prey items. The main arthropods caught were Hymenoptera (40-8%), Ephemeroptera (26-4%), Homoptera (15-4%) and Diptera (7-7%). Flying ants and mayflies contributed most by weight, although figwasps and mayflies were the most numerous prey items. Only in December did the percentage of moulting mist-netted adult swiftlets fall below 70 %. It appeared that many birds were moulting and breeding simultaneously, although 21% of birds had primaries moulting in two places, suggesting arrested moult. Diurnal activity showed a normal high dawn exodus and dusk inflow of birds, although there was evidence of an increase of birds flying out prior to the dusk inflow. Nest harvesting was continued throughout the study, but most of the marked nests under study were left undisturbed. 相似文献
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The Caco-2 cell model was used to study the efficiency of absorption and endogenous excretion of zinc (Zn) regulated by dietary Zn concentration. Cells were seeded onto high pore-density membranes and maintained in medium supplemented with 10% FBS. After confluence, cells were treated with 5 or 25 μmol Zn/L for 7 d, and Zn uptake and transport were measured in both apical (AP) and basolateral (BL) directions by using 65Zn. Similar cells were labeled with 65Zn and the release of Zn to the AP and BL sides was measured. The AP uptake of Zn in cells exposed to 25 μmol Zn/L was slower (p < 0.05) than that in cells exposed to 5 μmol Zn/L. The AP to BL transport rate in the 25 μmol Zn/L group was only 40% (p < 0.05) of that in the 5 μM group. In contrast, the rate of BL Zn uptake was 4-fold higher in cells treated with 25 μmol Zn/L than in those treated with 5 μmol Zn/L (p < 0.05). The BL to AP transport rate was 2-fold higher in cells treated with 25 μmol Zn/L than in those treated with 5 μmol Zn/L (p < 0.05). Basolateral uptake was 6 to 25 times greater (p < 0.05) than AP uptake for cells treated with 5 and 25 μmol Zn/L, respectively. The rate of Zn release was enhanced about 4-fold (p < 0.05) by 25 μmol Zn/L treatment. Release to the BL side was 10 times greater than to the AP side. Zn-induced metallothionein (MT), thought to down-regulate AP to BL Zn transport, was 4-fold higher (p < 0.001) in the 25 μmol Zn/L group than in the 5 μM group, but the rate of BL Zn release was higher in cells treated with 25 μmol Zn/L than in those treated with 5 μmol Zn/L (p < 0.05). Induced changes in transport rates by media Zn concentrations could involve the up- and/or down-regulation of Zn influx and efflux proteins such as the ZIP and ZnT families of Zn transporters. 相似文献
4.
Lin Yan Philip G. Reeves LuAnn K. Johnson 《Journal of trace elements in medicine and biology》2010,24(4):223-229
We assessed the bioavailability of selenium (Se) from a protein isolate and tofu (bean curd) prepared from naturally produced high-Se soybeans. The Se concentrations of the soybeans, the protein isolate and tofu were 5.2 ± 0.2, 11.4 ± 0.1 and 7.4 ± 0.1 mg/kg, respectively. Male weanling Sprague–Dawley rats were depleted of Se by feeding them a 30% Torula yeast-based diet (4.1 μg Se/kg) for 56 days, and then they were replenished with Se for an additional 50 days by feeding them the same diet containing 14, 24 or 30 μg Se/kg from the protein isolate or 13, 23 or 31 μg Se/kg from tofu, respectively. l-Selenomethionine (SeMet) was used as a reference. Selenium bioavailability was determined on the basis of the restoration of Se-dependent enzyme activities and tissue Se concentrations in Se-depleted rats, comparing those responses for the protein isolate and tofu to those for SeMet by using a slope-ratio method. Dietary supplementation with the protein isolate or tofu resulted in linear or log-linear, dose-dependent increases in glutathione peroxidase activities in blood and liver and in thioredoxin reductase activity in liver. Furthermore, supplementation with the protein isolate or tofu resulted in linear or log-linear, dose-dependent increases in the Se concentrations of plasma, liver, muscle and kidneys. These results indicated an overall bioavailability of approximately 101% for Se from the protein isolate and 94% from tofu, relative to SeMet. We conclude that Se from naturally produced high-Se soybeans is highly bioavailable in this model and that high-Se soybeans may be a good dietary source of Se. 相似文献
5.
Deletion 17q12 is a recurrent copy number variant that confers high risk of autism and schizophrenia
Moreno-De-Luca D;SGENE Consortium Mulle JG;Simons Simplex Collection Genetics Consortium Kaminsky EB Sanders SJ;GeneSTAR Myers SM Adam MP Pakula AT Eisenhauer NJ Uhas K Weik L Guy L Care ME Morel CF Boni C Salbert BA Chandrareddy A Demmer LA Chow EW Surti U Aradhya S Pickering DL Golden DM Sanger WG Aston E Brothman AR Gliem TJ Thorland EC Ackley T Iyer R Huang S Barber JC Crolla JA Warren ST Martin CL Ledbetter DH 《American journal of human genetics》2010,87(5):618-630
Autism spectrum disorders (ASD) and schizophrenia are neurodevelopmental disorders for which recent evidence indicates an important etiologic role for rare copy number variants (CNVs) and suggests common genetic mechanisms. We performed cytogenomic array analysis in a discovery sample of patients with neurodevelopmental disorders referred for clinical testing. We detected a recurrent 1.4 Mb deletion at 17q12, which harbors HNF1B, the gene responsible for renal cysts and diabetes syndrome (RCAD), in 18/15,749 patients, including several with ASD, but 0/4,519 controls. We identified additional shared phenotypic features among nine patients available for clinical assessment, including macrocephaly, characteristic facial features, renal anomalies, and neurocognitive impairments. In a large follow-up sample, the same deletion was identified in 2/1,182 ASD/neurocognitive impairment and in 4/6,340 schizophrenia patients, but in 0/47,929 controls (corrected p = 7.37 × 10−5). These data demonstrate that deletion 17q12 is a recurrent, pathogenic CNV that confers a very high risk for ASD and schizophrenia and show that one or more of the 15 genes in the deleted interval is dosage sensitive and essential for normal brain development and function. In addition, the phenotypic features of patients with this CNV are consistent with a contiguous gene syndrome that extends beyond RCAD, which is caused by HNF1B mutations only. 相似文献
6.
We have carried out molecular dynamics simulations of the naturally occurring protegrin PG-1 peptide and two of its mutants, PC-9 and PC-13 in the presence of a dodecyl-phosphocholine (DPC) micelle. The effects of mutations that disrupt the β-sheet structure in the case of PC-9 and reduce the charge at the C-terminus in the case of PC-13 are analyzed. It is found that the surface-bound conformations of the peptides are severely affected by both mutations. PG-1 exhibits a conformation in which the C-terminus and the β-hairpin turn interact strongly with the micelle lipid head groups, while its N-terminal strand bends away from the micelle and resides in the aqueous region; PC-13 exhibits strong interactions with the micelle at its N-terminus as well as the β-hairpin turn region, while retaining a much more compact conformation than PG-1; PC-9 achieves a highly distorted conformation relative to the homologous PG-1 structure, which allows both its termini and the β-hairpin region to interact with the micelle. These significant differences observed as a result of seemingly minor mutations to the sequences of the three peptides are explained in terms of the interplay between residue charges, structural rigidity and amphiphilic interactions. Conservative inferences are made bridging these biophysical interactions and the pharmacological profiles of the peptides. 相似文献
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Planting date effects on arthropod infestation and viral plant disease are undocumented for winter wheat, Triticum aestivum L., in South Dakota and the northern Great Plains. Winter wheat was planted over three dates (early, middle, and late; generally from late August to late September) to determine the effect on abundance of insect pests, incidence of plant damage, incidence of viral plant disease, and grain yield. The study was conducted simultaneously at two sites in South Dakota over three consecutive cropping seasons for a total of six site yr. Cereal aphids (Homoptera: Aphididae) were abundant in three site yr. Rhopalosiphum padi (L.), bird cherry-oat aphid, was the most abundant cereal aphid at the Brookings site, whereas Schizaphis graminum (Rondani), greenbug, predominated at Highmore. Aphid-days were greater in early versus late plantings. Aphid abundance in middle plantings depended on aphid species and site, but it usually did not differ from that in early plantings. Incidence of Barley yellow dwarf virus (family Luteoviridae, genus Luteovirus, BYDV) declined with later planting and was correlated with autumnal abundance of cereal aphids. Incidence of BYDV ranged from 24 to 81% among 1999 plantings and was < 8% in other years. Damage to seedling wheat by chewing insects varied for two site-years, with greater incidence in early and middle plantings. Wheat streak mosaic virus, spring infestations of cereal aphids, wheat stem maggot, and grasshoppers were insignificant. Yield at Brookings was negatively correlated with BYDV incidence but not cereal aphid abundance, whereas yield at Highmore was negatively correlated with aphid abundance but not BYDV incidence. Planting on 20 September or later reduced damage from chewing insects and reduced cereal aphid infestations and resulting BYDV incidence. 相似文献
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S Lekawanvijit AR Kompa M Manabe BH Wang RG Langham F Nishijima DJ Kelly H Krum 《PloS one》2012,7(7):e41281
Cardiovascular death commonly occurs in patients with chronic kidney disease. Indoxyl sulfate (IS), a uremic toxin, has been demonstrated in vitro as a contributory factor in cardiac fibrosis, a typical pathological finding in uremic cardiomyopathy. This study aimed to determine if cardiac fibrosis is reversible by lowering serum IS levels using an oral charcoal adsorbent, AST-120. Subtotal-nephrectomized (5/6-STNx) Sprague-Dawley rats were randomized to receive either AST-120 (AST-120, n=13) or no treatment (vehicle, n=17) for 12 weeks. Sham operated rats (n=12) were used as controls. Early left ventricular (LV) diastolic dysfunction was demonstrated by an increase in peak velocity of atrial filling [A and A' waves] and a decrease of E/A and E'/A' ratios obtained by echocardiography. This was accompanied by a 4.5-fold increase in serum IS (p<0.001) as well as elevated tail-cuff blood pressure (p<0.001) and heart weight (p<0.001). Increased LV fibrosis (p<0.001), gene expression of pro-fibrotic (TGF-β, CTGF) and hypertrophic (ANP, β-MHC and α-skeletal muscle actin) markers, as well as TGF-β and phosphorylated NF-κB protein expression were observed in STNx + vehicle rats. Treatment with AST-120 reduced serum creatinine (by 54%, p<0.05) and urine total protein (by 27%, p<0.05) vs vehicle whilst having no effect on blood pressure (AST-120=227 ± 11 vs vehicle =224 ± 8 mmHg, ns) and heart weight. The increase in serum IS was prevented with AST-120 (by 100%, p<0.001) which was accompanied by reduced LV fibrosis (68%, p<0.01) and TGF-β and phosphorylated NF-κB protein expression (back to sham levels, p<0.05) despite no significant change in LV function. In conclusion, STNx resulted in increased cardiac fibrosis and circulating IS levels. Reduction of IS with AST-120 normalizes cardiac fibrosis, in a blood pressure independent manner. 相似文献