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排序方式: 共有134条查询结果,搜索用时 46 毫秒
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Ricardo S. Bovendorp Fernanda T. Brum Robert A. McCleery Benjamin Baiser Rafael Loyola Marcus V. Cianciaruso Mauro Galetti 《Ecography》2019,42(1):23-35
Forest fragmentation and defaunation are considered the main drivers of biodiversity loss, yet the synergistic effects of landscape changes and biotic interactions on assemblage structure have been poorly investigated. Here, we use an extensive dataset of 283 assemblages and 105 species of small mammals to understand how defaunation of medium and large mammals and forest fragmentation change the community composition and diversity of rodents and marsupials in tropical forests of South America. We used structured equation models to investigate the relationship between small mammal species, functional and phylogenetic diversity with forest size, forest cover and the occurrence of medium and large mammals. The best‐fit model showed that defaunation reduced functional diversity, and that species diversity of small mammals increased with forest patch size. Forest cover did not affect functional and phylogenetic diversity. Our results indicate that occurrence of medium and large sized mammals (probably acting as predators, or competitors of small mammals) and forest patch size help to retain species and functional diversity in small mammal communities. Further, the number of species in a small mammal community was critical to the maintenance of phylogenetic diversity, and may have a pronounced influence on the ecological functions played by small mammals. Identifying how phylogenetic and functional diversity change in function of human pressures allows us to better understand the contribution of extant lineages to ecosystem functioning in tropical forests. 相似文献
3.
Lilian Patrícia Sales Bruno R. Ribeiro Mathias M. Pires Colin A. Chapman Rafael Loyola 《Ecography》2019,42(10):1789-1801
Climate change will redistribute the global biodiversity in the Anthropocene. As climates change, species might move from one place to another, due to local extinctions and colonization of new environments. However, the existence of permeable migratory routes precedes faunal migrations in fragmented landscapes. Here, we investigate how dispersal will affect the outcome of climate change on the distribution of Amazon's primate species. We modeled the distribution of 80 Amazon primate species, using ecological niche models, and projected their potential distribution on scenarios of climate change. Then, we imposed landscape restrictions to primate dispersal, derived from a natural biogeographical barrier to primates (the main tributaries of the Amazon river) and an anthropogenic constraint to the migration of many canopy‐dependent animals (deforested areas). We also highlighted potential conflict zones, i.e. regions of high migration potential but predicted to be deforested. Species response to climate change varied across dispersal limitation scenarios. If species could occupy all newly suitable climate, almost 70% of species could expand ranges. Including dispersal barriers (natural and anthropogenic), however, led to range expansion in only less than 20% of the studied species. When species were not allowed to migrate, all of them lost an average of 90% of the suitable area, suggesting that climate may become unsuitable within their present distributions. All Amazon primate species may need to move as climate changes to avoid deleterious effects of exposure to non‐analog climates. The effect of climate change on the distribution of Amazon primates will ultimately depend on whether landscape permeability will allow climate‐driven faunal migrations. The network of protected areas in the Amazon could work as ‘stepping stones’ but most are outside important migratory routes. Therefore, protecting important dispersal corridors is foremost to allow effective migrations of the Amazon fauna in face of climate change and deforestation. 相似文献
4.
Chaperone mediated autophagy contributes to the newly synthesized histones H3 and H4 quality control
Juan Hormazabal Francisco Saavedra Claudia Espinoza-Arratia Nicolas
W Martinez Tatiana Cruces Ivn
E Alfaro Alejandra Loyola 《Nucleic acids research》2022,50(4):1875
Although there are several pathways to ensure that proteins are folded properly in the cell, little is known about the molecular mechanisms regulating histone folding and proteostasis. In this work, we identified that chaperone-mediated autophagy (CMA) is the main pathway involved in the degradation of newly synthesized histones H3 and H4. This degradation is finely regulated by the interplay between HSC70 and tNASP, two histone interacting proteins. tNASP stabilizes histone H3 levels by blocking the direct transport of histone H3 into lysosomes. We further demonstrate that CMA degrades unfolded histone H3. Thus, we reveal that CMA is the main degradation pathway involved in the quality control of histone biogenesis, evidencing an additional mechanism in the intricate network of histone cellular proteostasis. 相似文献
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Eder Jorge de Oliveira Vanusia Batista Oliveira Amorim Edneide Luciana Santiago Matos Juliana Leles Costa Milene da Silva Castellen Juliano Gomes Pádua Jorge Luiz Loyola Dantas 《Plant Molecular Biology Reporter》2010,28(3):519-530
A set of 81 new microsatellite markers for Carica papaya L. previously identified by data mining using freely available sequence information from Genbank were tested for polymorphism
using 30 germplasm accessions from the Papaya Germplasm Bank (PGM) at Embrapa Mandioca e Fruticultura Tropical (CNPMF) and
18 landraces. The data were used to estimate pairwise genetic distances between the genotypes. A neighbor-joining based dendrogram
was used to define clusters and infer possible genetic structuring of the collection. Most microsatellites were polymorphic
(73%), with an observed number of alleles per locus ranging from one to eleven. The levels of observed and expected heterozygosity
for 51 polymorphic loci varied from 0.00 to 0.85 and from 0.08 to 0.82, averaging 0.19 and 0.59, respectively. Forty-four
percent of microsatellites showed polymorphism information content (PIC) higher than 0.50. The compound microsatellites seem
to be more informative than dinucleotide and trinucleotide repeats in average alleles per locus and PIC. Among dinucleotides,
AG/TC or GA/CT repeat motifs exhibited more informativeness than TA/AT, GT/CA and TG/AC repeat motifs. The neighbor-joining
analysis based on shared allele distance could differentiate all the papaya accessions and landraces as well as differences
in their genetic structure. This set of markers will be useful for examining parentage, inbreeding and population structure
in papaya. 相似文献
7.
Conchon-Costa I Loyola W Gaziri LC Custódio LA Felipe I 《FEMS immunology and medical microbiology》2007,49(3):330-336
The mechanisms through which Candida albicans is recognized by immune cells and how it triggers host defence are not completely understood. In this study, we evaluated the effect of Concanavalin-A on the clearance of C. albicans by infected mice and their production of proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). Subgroups of 5 animals were pretreated with Con-A (250 mug mL(-1) PBS) and after 96 h were infected intraperitoneally with 10(7) cells of C. albicans CR15 (an isolate from a HIV+ person); 30 min, 2, 6, 24 or 72 h after infection the mice were sacrificed. Phagocytosis of C. albicans by peritoneal macrophages increased 30 min after infection in mice pretreated with Con-A. The liver presented the greatest number of CFUs, and this number was reduced by pretreatment with Con-A. Control animals infected with C. albicans presented a significant increase in plasmatic alanine aminotransferase, which was not observed in mice treated with Con-A. Two hours after infection the production of TNF-alpha in the liver of mice pretreated with Con-A was significantly increased. These results suggest that a single dose of Con-A caused a beneficial modulating action of the inflammatory response during infection with C. albicans. 相似文献
8.
David E Loyola Cristell Navarro Paulina Uribe Katherine García Claudia Mella Diego Díaz Natalia Valdes Jaime Martínez-Urtaza Romilio T Espejo 《BMC genomics》2015,16(1)
Background
New strains of Vibrio parahaemolyticus that cause diarrhea in humans by seafood ingestion periodically emerge through continuous evolution in the ocean. Influx and expansion in the Southern Chilean ocean of a highly clonal V. parahaemolyticus (serotype O3:K6) population from South East Asia caused one of the largest seafood-related diarrhea outbreaks in the world. Here, genomics analyses of isolates from this rapidly expanding clonal population offered an opportunity to observe the molecular evolutionary changes often obscured in more diverse populations.Results
Whole genome sequence comparison of eight independent isolates of this population from mussels or clinical cases (from different years) was performed. Differences of 1366 to 217,729 bp genome length and 13 to 164 bp single nucleotide variants (SNVs) were found. Most genomic differences corresponded to the presence of regions unique to only one or two isolates, and were probably acquired by horizontal gene transfer (HGT). Some DNA gain was chromosomal but most was in plasmids. One isolate had a large region (8,644 bp) missing, which was probably caused by excision of a prophage. Genome innovation by the presence of unique DNA, attributable to HGT from related bacteria, varied greatly among the isolates, with values of 1,366 (ten times the number of highest number of SNVs) to 217,729 (a thousand times more than the number of highest number of SNVs).Conclusions
The evolutionary forces (SNVs, HGT) acting on each isolate of the same population were found to differ to an extent that probably depended on the ecological scenario and life circumstances of each bacterium.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1385-8) contains supplementary material, which is available to authorized users. 相似文献9.
Arolas JL D'Silva L Popowicz GM Aviles FX Holak TA Ventura S 《Structure (London, England : 1993)》2005,13(8):1193-1202
The III-A intermediate constitutes the major rate-determining step in the oxidative folding of leech carboxypeptidase inhibitor (LCI). In this work, III-A has been directly purified from the folding reaction and structurally characterized by NMR spectroscopy. This species, containing three native disulfides, displays a highly native-like structure; however, it lacks some secondary structure elements, making it more flexible than native LCI. III-A represents a structurally determined example of a disulfide-insecure intermediate; direct oxidation of this species to the fully native protein seems to be restricted by the burial of its two free cysteine residues inside a native-like structure. We also show that theoretical approaches based on topological constraints predict with good accuracy the presence of this folding intermediate. Overall, the derived results suggest that, as it occurs with non-disulfide bonded proteins, native-like interactions between segments of secondary structure rather than the crosslinking of disulfide bonds direct the folding of LCI. 相似文献
10.
José Alexandre Felizola Diniz-Filho Dayane Borges Melo Guilherme de Oliveira Rosane Garcia Collevatti Thannya Nascimento Soares Jo?o Carlos Nabout Jacqueline de Souza Lima Ricardo Dobrovolski Lázaro José Chaves Ronaldo Veloso Naves Rafael Dias Loyola Mariana Pires de Campos Telles 《Conservation Genetics》2012,13(4):1085-1093
Systematic Conservation Planning (SCP) involves a series of steps that should be accomplished to determine the most cost-effective way to invest in conservation action. Although SCP has been usually applied at the species level (or hierarchically higher), it is possible to use alleles from molecular analyses at the population level as basic units for analyses. Here we demonstrate how SCP procedures can be used to establish optimum strategies for in situ and ex situ conservation of a single species, using Dipteryx alata (a Fabaceae tree species widely distributed and endemics to Brazilian Cerrado) as a case study. Data for the analyses consisted in 52 alleles from eight microsatellite loci coded for a total of 644 individual trees sampled in 25 local populations throughout species’ geographic range. We found optimal solutions in which seven local populations are the smallest set of local populations of D. alata that should be conserved to represent the known genetic diversity. Combining these several solutions allowed estimating the relative importance of the local populations for conserving all known alleles, taking into account the current land-use patterns in the region. A germplasm collection for this species already exists, so we also used SCP approach to identify the smallest number of populations that should be further collected in the field to complement the existing collection, showing that only four local populations should be sampled for optimizing the species ex situ representation. The initial application of the SCP methods to genetic data showed here can be a useful starting point for methodological and conceptual improvements and may be a first important step towards a comprehensive and balanced quantitative definition of conservation goals, shedding light to new possibilities for in situ and ex situ designs within species. 相似文献