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1.
We isolated a recombinant H9N2 avian influenza virus (AIV) from fresh egret feces in the Ardeidae protection region of the Dongting Lake wetland area in China, and it was designated A/Egret/Hunan/1/2012(H9N2). This is the first report of isolating H9N2 AIV from wild birds in the Dongting Lake wetland. Its eight gene segments are generated by reassortment of gene segments of different AIV subtypes. These results are helpful for understanding the epidemiology and evolution of AIV in wild birds during migration. 相似文献
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There has been a long debate on the source of temporal fluctuations in natural population densities. The difficulty is that unpredictable irregularities might be attributed either to external environmental factors or to chaotic dynamics of populations, or even to the interaction of these two factors. Some years ago Cohen (1995) pointed out that real time series follow redshifted Fourier power spectra, while the simplest chaotic population dynamical models are mostly blueshifted. Since then, the controversy has focused on comparisons of Fourier spectra originating from different models and data. Here, we show experimentally that estimation process by human observers shifts power spectra to the red. This result implies that because of estimation distortion, real population data must be less redshifted than many recorded time series suggest. 相似文献
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棕色固氮菌缺失nifZ基因的突变种固氮酶MoFe蛋白的纯化和性质 总被引:1,自引:0,他引:1
采用 52℃下加热 6 min,后经 DEAE- 52、Sephacryls S- 2 0 0和 Q- Sepharose等柱层析方法 ,分离纯化了棕色固氮菌 (Azotobacter vinelandii)缺失 nif Z基因突变种固氮酶 Mo Fe(Δnif Z Mo Fe)蛋白 ,其纯度达到电泳纯。Δnif Z Mo Fe蛋白的固氮活性为 2 83nmol C2 H2 还原 / (min·mg蛋白 ) ,远低于野生种 Mo Fe蛋白。Δnif Z Mo Fe蛋白对氧更敏感 ;热稳定性略低于野生种。Δnif Z Mo Fe蛋白的可见光吸收光谱与野生种 Mo Fe蛋白极为相似。其圆二色谱和磁圆二色谱在 450~ 550 nm与野生种 Mo Fe蛋白显著不同 ,表明其 P- cluster及其周围环境与野生种 Mo Fe蛋白有所差异。这亦可能是造成缺失 nif Z突变种 Mo Fe蛋白固氮活性低的原因。 相似文献
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Yangxun Pan Deyao Zhang Yuheng Chen Huake Li Jiongliang Wang Ze Yuan Liyang Sun Zhongguo Zhou Minshan Chen Yaojun Zhang Dandan Hu 《Journal of cellular and molecular medicine》2023,27(7):1006-1020
Hepatocellular carcinoma (HCC) is one of the most common malignant tumours worldwide. Given metabolic reprogramming in tumours was a crucial hallmark, several studies have demonstrated its value in the diagnostics and surveillance of malignant tumours. The present study aimed to identify a cluster of metabolism-related genes to construct a prediction model for the prognosis of HCC. Multiple cohorts of HCC cases (466 cases) from public datasets were included in the present analysis. (GEO cohort) After identifying a list of metabolism-related genes associated with prognosis, a risk score based on metabolism-related genes was formulated via the LASSO-Cox and LASSO-pcvl algorithms. According to the risk score, patients were stratified into low- and high-risk groups, and further analysis and validation were accordingly conducted. The results revealed that high-risk patients had a significantly worse 5-year overall survival (OS) than low-risk patients in the GEO cohort. (30.0% vs. 57.8%; hazard ratio [HR], 0.411; 95% confidence interval [95% CI], 0.302–0.651; p < 0.001) This observation was confirmed in the external TCGA-LIHC cohort. (34.5% vs. 54.4%; HR 0.452; 95% CI, 0.299–0.681; p < 0.001) To promote the predictive ability of the model, risk score, age, gender and tumour stage were integrated into a nomogram. According to the results of receiver operating characteristic curves and decision curves analysis, the nomogram score possessed a superior predictive ability than conventional factors, which indicate that the risk score combined with clinicopathological features was able to achieve a robust prediction for OS and improve the individualized clinical decision making of HCC patients. In conclusion, the metabolic genes related to OS were identified and developed a metabolism-based predictive model for HCC. Through a series of bioinformatics and statistical analyses, the predictive ability of the model was approved. 相似文献
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干燥番茄(Lycopersicon esculentum Mill. cv. Moneym aker)种子浸种吸水有明显的3 个阶段,尽管种子不同部分吸水的速度不一样,但整粒种子需10~12 h 才能基本完成第一阶段的吸水。胚乳对种胚伸长和生长的机械压力明显阻碍种胚的吸水,即使在第二阶段整个种子水势与浸种溶液基本达到水分平衡时,种胚的水势仍然低于整个种子水势0.6~0.9 MPa。发芽过程中GA 和ABA不直接影响番茄种子吸水。番茄种胚水势与含水量的关系呈幂指数的正相关。无论在浸种还是渗控处理过程中,番茄种胚压力势的总变化趋势是下降的 相似文献
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Two kinds of responses to cholinomimetics were found on the identified neuron (P-2) of Planorbarius corneus pedal ganglion using microelectrode technique. Nicotinomimetics caused depolization whereas some muscarinomimetics caused hyperpolarization of the neuron membrane. Acetylcholine usually depolarized the neuron membrane but after the blockade of nicotinic receptors with tubocurarine one can reveal a hyperpolarizing action of acetylcholine. These findings suggest that two kinds of receptors exist on the P-2 neuron membrane and these receptors differ both in pharmacological characteristics and in ionic permeability changes they control. 相似文献
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风力发电减碳效益评估有助于从减碳角度更好制定能源发展相关政策。以风力资源总体丰富且亟需发展风力发电以实现能源系统脱碳的新疆为研究区,将生命周期方法与风力发电模型结合,在省、市级尺度分别评估了风力发电全生命周期的排放水平及发电效益,核算了风力发电相对于火力发电和光伏发电的减碳效益,有效弥补了传统生命周期评估中空间差异考虑不充分的问题。结果表明,风机全生命周期平均发电量为13.1×107 kWh,风力发电全生命周期共排放3944.24 tCO2-eq,通过材料处置回收和循环再利用可减少1424.79 tCO2-eq。新疆发展风力发电具有低排放强度和高减碳效益的特点,与火电相比可减少97.44%排放,减碳效益平均可达906.72 gCO2-eq/kWh,并且应优先布局在哈密、巴音郭楞蒙古自治州和北屯市;与光伏相比,减碳效益可分别达到43.85 gCO2-eq/kWh(衰减率DR=1%)和169.84 gCO2-eq/kWh(DR=3%),此情景下风电应主要部署在克孜勒苏柯尔克孜自治州、喀什和和田。在风电减碳效益较差地区如石河子市、铁门关市和双河市应考虑利用本地充足太阳能资源发展光伏发电。需注意风电的排放强度和减碳效益在局地小尺度评估中存在不确定性,获取更精细的结果仍需进一步评估。未来应大力发展新疆本地的风电产业,打造绿色供应链和加快发展处置回收技术以增加减碳效益。 相似文献