Effects of isoproterenol,theophylline and carbachol on cyclic nucleotide levels and relaxation of bovine tracheal smooth muscle

The effect of theophylline and isoproterenol on bovine tracheal smooth muscle tension and cyclic AMP levels was investigated. Concentrations of isoproterenol (4 × 10^?6 M) and theophylline (10 mM) that relaxed carbachol-contracted tracheal muscle by 85–95% did not significantly elevate control levels of cyclic AMP. In the absence of carbachol, several-fold increases in cyclic AMP were caused by isoproterenol although no elevations by theophylline were measurable. However, when isoproterenol and theophylline were administered together, theophylline potentiated the rise in cyclic AMP caused by isoproterenol. Phosphodiesterase studies in tracheal muscle showed the presence of a high and a low K_m enzyme which were inhibited by theophylline. Cyclic GMP levels were elevated in muscles contracted by carbachol as well as in carbachol-contracted muscles that had been relaxed by theophylline. In non-tension studies, in which the tracheal muscle was not under isometric tension, carbachol or theophylline alone increased cyclic GMP and together they synergistically elevated cyclic GMP. Atropine blocked the elevation caused by carbachol but not that caused by theophylline. In contrast to theophylline, isoproterenol did not elevate cyclic GMP, and in carbachol-contracted muscles that had been relaxed by isoproterenol, cyclic GMP levels were no different from control. Also, in non-tension studies, isoproterenol decreased basal cyclic GMP and antagonized the increase in cyclic GMP due to carbachol.The results indicate that whole-tissue levels of cyclic AMP and cyclic GMP do not correlate with the state of tracheal smooth muscle tension. Cyclic GMP levels do not clearly correlate with either contraction or relaxation. The inhibition by carbachol of increases in cyclic AMP due to isoproterenol and the inhibition by isoproterenol of increases in cyclic GMP due to carbachol provide evidence for a reciprocal cholinergic-adrenergic antagonism of cyclic AMP and cyclic GMP levels. The antagonism did not appear to be due to either cyclic nucleotide affecting the elevation of the other since the levels of both cyclic nucleotides were depressed.     

Nährstoffwirkung und Giftwirkung bei Aspergillus niger.

Ohne Zusammenfassung     

Are there insertions in the ribosomal DNA of vertebrates?

We have analysed the Eco RI restriction pattern of rDNA of the newt Triturus vulgaris and of some other amphibian species by Southern blotting and hybridization with nick-translated Xenopus rDNA prepared from the recombinant plasmids pXlr11 and pXlr12 (21). After hybridization with r11, the 28S coding fragments become visible in two bands, a prominent one of 5.3 kb and a weak band of 5.9 kb representing about 8% of the 28S genes. The evidence obtained so far by additional digestions with Bam HI and Bgl II indicates that in this species and in Triturus helveticus the coding regions of the 5.9 kb fragments are interrupted by an insertion 0.6 kb in length located in a 1.6 kb Bgl II fragment at the 3' end of the Eco RI fragment, which we believe to be the first described in a vertebrate.     

Influence of Phospholipids on Growth, Sporulation and Virulence of the Endoparasitic Fungi Drechmeria coniospora, Verticillium balanoides and Harposporium anguillulae in Liquid Culture

Verticillium balanoides mycelial growth was stimulated on solid corn meal agar (1.7 %) and in liquid corn meal broth (0.2 %) upon the addition of phospholipids at various concentrations. Sporulation differed with phospholipid products and was highest in pure corn meal. Drechmeria coniospora mycelial growth increased upon addition of phospholipids at all concentrations in solid or liquid culture. Sporulation increased at high concentration (1000 ppm) and decreased at low concentration (100 ppm) of phospholipids in the medium. For both fungi, infectivity of conidia produced in liquid culture decreased when compared to conidia from parasitized nematodes. Addition of phospholipids partly restored this effect. Harposporium anguillulae mycelial growth and sporulation was not affected by addition of phospholipids to solid or liquid corn meal medium.     

A neural network model for the prediction of membrane-spanning amino acid sequences

The architecture and weights of an artificial neural network model that predicts putative transmembrane sequences have been developed and optimized by the algorithm of structure evolution. The resulting filter is able to classify membrane/nonmembrane transition regions in sequences of integral human membrane proteins with high accuracy. Similar results have been obtained for both training and test set data, indicating that the network has focused on general features of transmembrane sequences rather than specializing on the training data. Seven physicochemical amino acid properties have been used for sequence encoding. The predictions are compared to hydrophobicity plots.     

银杉遗传多样性的RAPD分析

用随机扩增多态 DNA(RAPD)标记方法对银杉(Chthaya argyrophylla)75个个体(采自湖南和四川)进行了遗传多样性检测. 21个 10 mer -的寡核苷酸引物共检测106个位点,其中多态位点34个,占32％.相对于其它裸子植物,银杉的遗传变异水平偏低.湖南居群和四川居群的多态位点百分率分别为 18％和 25％,两居群间的遗传变异量占总变异量的 7. 99％,这一数值高于裸子植物居群间遗传差异的平均值(6.8％).同时,发现遗传变异水平的高低与生境的复杂程度有一定的相关性.由于点突变和随机遗传漂变,银杉的部分亚居群间有较强烈的分化,亚居群间的遗传差异最高可达 16. 23％.此外,提出了度量遗传多样性水平的分化指数概念及其计算方法,并指出低水平的遗传多样性可能是银杉濒危的原因之一.     

Oceanographic significance of Pacific late miocene calcareous nannoplankton

An analysis of the variability in the composition and distribution of Pacific Late Miocene calcareous nannoplankton about their average biogeography shows that there are primarily two environmental factors causing that variability, climate and dissolution. Climate produces a latitudinal, biogeographic differentiation of the Late Miocene nannoflora, while selective dissolution superimposes a bathymetric differentiation of the nannoflora on that due to climate. Together, these two factors produce three distinct Late Miocene nannofloral assemblages, a high-latitude, temperate assemblage characterized by Reticulofenestra pseudoumbilica and Coccolithus pelagicus, and two tropical assemblages, their differences in composition depending on water depth and surface-water productivity: (1) in shallower water and beneath areas of higher organic production and sedimentation of calcite there is an undissolved assemblage characterized by sphenoliths, small elliptical placoliths and Coccolithus pataecus; (2) in deeper water and areas of lower productivity there is a dissolved assemblage dominated by discoasters.Selective dissolution produces most of the apparent biogeographic variation in Pacific Late Miocene nannoplankton compositions, the variation in compositions observed between the seventeen sites studied. Dissolution preferentially removes the more soluble constituents of the tropical nannoflora so that increasing dissolution tends to give tropical nannoflora a cooler, more temperate aspect. At the same time, selective dissolution shifts the composition of the warmer, tropical component towards its more resistant taxa.Nannoplankton records show a period of greatly decreased calcite dissolution in deep tropical and temperate South Pacific sites between about 8 and 10 m.y. ago. This decrease is strongly correlated with a temporary increase in the ¹³C composition of Pacific deep waters. Calcite dissolution increased during this same period in the deep North Pacific.Nannoplankton records of Late Miocene climate in the tropics are distinctly different from those at higher, south temperate latitudes. Tropical records show a sharp warming in the earliest Late Miocene after a generally cool late Middle Miocene. This was followed by a temporary cooling, nearly to Middle Miocene levels, about 7 m.y. ago. Toward the end of the Late Miocene, the tropical Pacific warmed again and remained warm into the Pliocene. Warming of temperate climates occurred much later. Not until latest Miocene did the southern the Pliocene. Warming of temperate climates occurred much later. Not until latest Miocene did the southern temperate latitudes warm appreciably. Southern subpolar climate cooled continuously through the Late Miocene. We attribute the resulting increases in the latitudinal climatic contrast across the southern Pacific Ocean to the development and migration of a strong subtropical convergence.On the basis of the nannoplankton oceanographic records we postulate that beginning about 10.5 m.y. ago Pacific surface circulation became primarily zonal and the production of deep and bottom waters in the Southern Ocean increased sharply. This produced a northward decrease in calcite preservation, an increase in benthic ¹³C, and a strong climatic gradient across southern latitudes. The period of most vigorous deep Pacific circulation ended 7 m.y. ago in response, we speculate, to the reduced ocean salinities during the Messinian.     

Chick embryo muscarinic and purinergic receptors activate cytosolic Ca2+ via phosphatidylinositol metabolism

In dissociated cells from chick embryos or from chick limb buds, acetylcholine (ACh) induced an increase in cellular levels of inositol 1,4,5-trisphosphate (Ins-P3) and of inositol 1,3,4,5-tetrakisphosphate (Ins-P4). The concentration of Ins-P3 was enhanced transiently, whereas the level of Ins-P4 remained elevated for at least 20 min after addition of ACh. In most cases the increase in Ins-P4 levels was more pronounced than that of Ins-P3 levels. The inhibition of the ACh-induced inositol-phosphate response by atropine (half-maximal inhibition at 10 nM) indicates the involvement of muscarinic receptors, which in chick embryo cells induce a transient rise and a following persistent elevation of cytosolic Ca2+ activity (G. Oettling et al. (1989) J. Dev. Physiol. 12, 85-94). Adenosine 5'-triphosphate (ATP) elicited a similar transient rise in cytosolic Ca2+ activity, however, without a subsequent plateau. ATP also caused an increase in inositol-oligophosphate levels. Thus, both muscarinic and purinergic receptors in chick embryo cells are coupled to phospholipase C. The enzymatically formed Ins-P3 mediates the release of Ca2+ from internal stores. The Ca2+ signal could be involved in embryonic cell migration during morphogenesis.