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排序方式: 共有93条查询结果,搜索用时 187 毫秒
1.
Nguyen Hoang Loc Le My Tieu Ngoc Tran Thuy Lan Le Quoc Viet Le Duc Thao Hoang Tan Quang Dinh Thi Bich Lan Phung Thang Long 《Indian journal of microbiology》2013,53(4):488-491
We cloned two genes coding F107-C and K88-1NT fimbrial subunits from strains E. coli C and 1NT isolated from Thua Thien Hue province, Vietnam. The mature peptide of faeG gene from strain E. coli 1NT (called faeG-1NT) is 100 % similarity with faeG gene, while the CDS of fedA gene from strain C (called fedA-C) has a similarity of 97 % with the fedA gene. Expression of the faeG-1NT and fedA-C genes in E. coli BL21 Star™ (DE3) produced proteins of ~31 and 22 kDa, respectively. The effect of IPTG concentration on the K88-1NT and F107-C fimbriae production was investigated. The results showed that 0.5 mM IPTG is suitable for higher expression of K88-1NT subunit, while 0.75 mM IPTG strongly stimulated expression of F107-C subunit. The optimal induction time for expression was also examined. Generally, highest expression of K88-1NT subunit occurred after 6 h of induction, while that of F107-C subunit is after 14 h. 相似文献
2.
Jae-Hwa?Lee Nguyen-Hoang?Loc Tae-Ho?Kwon Moon-Sik?YangEmail author 《Biotechnology and Bioprocess Engineering》2004,9(1):12-16
Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems
(ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene
glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium
phosphate systems on the partition coefficient,K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system,
hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% andK
hGM-CSF of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase
with a yield of 45.66% andK
hGM-CSF of 7.64 after 2 h of incubation at room temperature. 相似文献
3.
Bacteriophage specific for Campylobacter were isolated from chicken excreta collected from established free-range layer breed stock. Bacteriophage were either propagated on a Campylobacter jejuni host with broad susceptibility to bacteriophage (NCTC 12662) or on Campylobacter isolates from the same samples. Campylobacters were confirmed as being C. jejuni and or C. coli, using a combination of standard biochemical tests and PCR analysis with genus and species specific primers. The bacteriophage displayed differential patterns of susceptibility against reference NCTC strains and contemporary C. jejuni /C. coli isolates from chicken excreta. Electron microscopy demonstrated that the phage possessed icosahedral heads and rigid contractile tails. Pulsed-field gel electrophoresis revealed the bacteriophage genomes to be double stranded DNA in the range of 140 kb in size and the restriction enzyme patterns of the DNAs indicate they are genetically related members of the Myoviridae family. This study showed that Campylobacter bacteriophage could easily be isolated from free-range chickens and form part of their normal microbiological biota of environmentally exposed birds. 相似文献
4.
Nguyen Hoang Loc Le Thi Thinh Moon-Sik Yang Tae-Geum Kim 《Biotechnology and Bioprocess Engineering》2011,16(3):576-580
The cholera toxin B subunit (CTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral
adjuvant that induces a strong immune response against co-administered or coupled antigens. A gene encoding CTB, which was
modified based on the optimized codon usage in the plant, was synthesized and fused to the endoplasmic reticulum retention
signal KDEL to enhance its expression level in plants. The synthetic CTB (sCTB) gene was introduced into a plant expression
vector adjacent to the CaMV 35S promoter, and was transformed into tomato using an Agrobacterium-mediated transformation method. The integration of the sCTB gene into the genomic DNA of transgenic plants was confirmed
by genomic DNA PCR amplification. The synthesis and assembly of CTB protein in transgenic plants was demonstrated through
immunoblot analysis and GM1-ELISA. The highest amount of CTB protein produced in transgenic tomatoes was approximately 0.9% of total soluble fruit protein
which was 10-fold greater than the previously 0.081%. GM1-ELISA indicated that plant-synthesized CTB protein bound specifically to GM1-gangliosides, suggesting that the CTB subunits formed active pentamers. 相似文献
5.
Nguyen HP Hanson J Bethell D Nguyen TH Tran TH Ly VC Pham PL Dinh XS Dondorp A White N Tran TH Day N 《PloS one》2011,6(10):e25523
Background
Optimising the fluid resuscitation of patients with severe malaria is a simple and potentially cost-effective intervention. Current WHO guidelines recommend central venous pressure (CVP) guided, crystalloid based, resuscitation in adults.Methods
Prospectively collected haemodynamic data from intervention trials in Vietnamese adults with severe malaria were analysed retrospectively to assess the responses to fluid resuscitation.Results
43 patients were studied of whom 24 received a fluid load. The fluid load resulted in an increase in cardiac index (mean increase: 0.75 L/min/m2 (95% Confidence interval (CI): 0.41 to 1.1)), but no significant change in acid-base status post resuscitation (mean increase base deficit 0.6 mmol/L (95% CI: −0.1 to 1.3). The CVP and PAoP (pulmonary artery occlusion pressure) were highly inter-correlated (rs = 0.7, p<0.0001), but neither were correlated with acid-base status (arterial pH, serum bicarbonate, base deficit) or respiratory status (PaO2/FiO2 ratio). There was no correlation between the oxygen delivery (DO2) and base deficit at the 63 time-points where they were assessed simultaneously (rs = −0.09, p = 0.46).Conclusions
In adults with severe falciparum malaria there was no observed improvement in patient outcomes or acid-base status with fluid loading. Neither CVP nor PAoP correlated with markers of end-organ perfusion or respiratory status, suggesting these measures are poor predictors of their fluid resuscitation needs. 相似文献6.
Grisshammer R White JF Trinh LB Shiloach J 《Journal of structural and functional genomics》2005,6(2-3):159-163
Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies,
require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression
and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound
ligands. 相似文献
7.
Charlat S Hornett EA Dyson EA Ho PP Loc NT Schilthuizen M Davies N Roderick GK Hurst GD 《Molecular ecology》2005,14(11):3525-3530
Male-killing bacteria are generally thought to attain low to intermediate prevalence in natural populations, with only mild effects on the host population sex ratio. This view was recently challenged by reports of extremely high infection frequencies in three butterfly species, raising the prospect that male killers, by making males rare, might drive many features of host ecology and evolution. To assess this hypothesis, it is necessary to evaluate how often male killers actually produce a highly female-biased population sex ratio in nature, which requires both high prevalence of infection and high penetrance of action. To this end, we surveyed South Pacific and Southeast Asian populations of Hypolimnas bolina, a butterfly in which extreme prevalence of male-killing Wolbachia bacteria has recently been recorded. Our results indicate that highly female-biased populations are common in Polynesia, with 6 out of 12 populations studied having in excess of 70% of females infected with a fully efficient male killer. However, heterogeneity is extreme in Polynesia, with the male-killing Wolbachia absent from three populations. In contrast to the Polynesian situation, Wolbachia does not kill males in any of the three Southeast Asian populations studied, despite its very high prevalence there. We conclude that male killers are likely to have significant ongoing ecological and evolutionary impact in 6 of the 15 populations surveyed. The causes and consequences of the observed spatial variation are discussed with respect to host resistance evolution, host ecology and interference with additional symbionts. 相似文献
8.
An experimental malaria transmission blocking vaccine antigen, Pfs25H, expressed and secreted from Pichia pastoris was recovered and purified using a screenless expanded bed column equipped with a rotating fluid distribution system. This column was able to accommodate feed stock, containing 30% biomass, at a flow rate of 300–400 cm/h without affecting column stability. This capability is three times higher than the capability of the expanded bed column currently in use, which is equipped with a perforated plate fluid distribution system; this design could accommodate biomass concentrations of only up to 10%. The screen-less design did not affect the binding capacity, purification level or process yield and, therefore, shorten the process. Purified Pfs25H of 6.4 g were recovered from 37 l of Pichia pastoris culture in one step. 相似文献
9.
10.
Nguyen Hoang Loc Vo Chau Tuan Doan Huu Nhat Binh Truong Thi Bich Phuong Tae-Geum Kim Moon-Sik Yang 《Biotechnology and Bioprocess Engineering》2009,14(5):619-624
We developed a cell suspension culture system for zedoary (Curcuma zedoaria Roscoe), using 100 g fresh weight inoculum in a batch culture. The maximum cell biomass of 68.46 g/L fresh weight was obtained
after 14 days of culture in a 10 L bioreactor with a pitch-blade impeller maintained at an agitation speed of 150 rpm and
an aeration rate of 2.5 L/min. The accumulation of sesquiterpenes and polysaccharide in zedoary cells from 2 to 18 days was
measured by HPLC and a phenol-sulfuric acid assay, respectively. The total polysaccharide concentration increased between
2 to 10 days of culture and reached a maximum value of 6.55%. HPLC revealed several eluted peaks of sesquiterpenes, which
increased in amplitude from days 2 to 10. Furthermore, our results indicated that biotransformation occurred in the cell suspension,
transforming certain sesquiterpenes into other types during culture. 相似文献