Protease-Dead Separase Is Dominant Negative in the C. elegans Embryo

Separase is a protease that promotes chromosome segregation at anaphase by cleaving cohesin. Several non-proteolytic functions of separase have been identified in other organisms. We created a transgenic C. elegans line that expresses protease-dead separase in embryos to further characterize separase function. We find that expression of protease-dead separase is dominant-negative in C. elegans embryos, not previously reported in other systems. The C. elegans embryo is an ideal system to study developmental processes in a genetically tractable system. However, a major limitation is the lack of an inducible gene expression system for the embryo. We have developed two methods that allow for the propagation of lines carrying dominant-negative transgenes and have applied them to characterize expression of protease-dead separase in embryos. Using these methods, we show that protease-dead separase causes embryo lethality, and that protease-dead separase cannot rescue separase mutants. These data suggest that protease-dead separase interferes with endogenous separase function, possibly by binding substrates and protecting them from cleavage.     

Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger

We have isolated AE3, a novel gene expressed primarily in brain neurons and in heart. The predicted AE3 polypeptide shares a high degree of identity with the anion exchange and cytoskeletal binding domains of the erythrocyte band 3 protein. Expression of AE3 cDNA in COS cells leads to chronic cytoplasmic acidification and to chloride- and bicarbonate-dependent changes in intracellular pH, confirming that this gene product is an anion exchanger. Characterization of an AE3 mutant lacking the NH2-terminal 645 amino acids demonstrates that the COOH-terminal half of the polypeptide is both necessary and sufficient for correct insertion into the plasma membrane and for anion exchange activity. The NH2-terminal domain may play a role in regulating the activity of the exchanger and may be involved in the structural organization of the cytoskeleton in neurons.     

Ankylosis of hock joints in group caged male B6C3F1 mice

Enlarged hock joints were observed during 1983 in B6C3F1 mice of chronic toxicity and carcinogenicity studies sponsored by the National Toxicology Program (NTP). Subsequently, approximately 9,500 B6C3F1 mice on 32 NTP chemical toxicity and carcinogenicity studies were evaluated for this condition by clinical examination. Group caged male B6C3F1 mice had thickening and reduced mobility of the hock joints at prevalences of 1.2% up to 6 months of age; 23% at 6 to 12 months of age; and 62% at 13 to 26 months of age. Group caged female B6C3F1 mice had a prevalence of 2% or less. Histologically, affected mice had periarticular exostoses on the bones of the hock joints, with formation of bony bridges around joints and deposition of new bone in joint spaces, resulting in partial or complete ankylosis. Individually caged male and female B6C3F1 mice were not affected. The cause of the ankylosis was not determined, but its occurrence in the NTP studies has been reduced by individual caging.     

The effect of grasses on the quality of transmitted radiation and its influence on the growth of white clover Trifolium repens

Summary Plants of white clover Trifolium repens were grown under the canopies of three grass species, Lolium perenne, Agrostis tenuis and Holcus lanatus, and under simulated canopies of black polythene and controls were exposed to unfiltered natural radiation. The canopies were adjusted so that they transmitted equal intensities of Photosynthetically Active Radiation (P.A.R.). The ratio of red to far red radiation () was unchanged under the black polythene canopies but was reduced under canopies of Lolium and Agrostis and even more so under Holcus. The effect of canopy filtered radiation on the growth of clover was greatly to reduced internode length, mean number of nodes, the number of branched nodes and the number of rooted nodes and greatly to increase petiole length. The effect of canopies of Holcus was greater than that of the other grass species both in its effect on and on the responses of the clover plant to its shade.     

A marine and terrestrial Sirius Group succession, middle Beardmore Glacier-Queen Alexandra Range, Transantarctic Mountains, Antarctica

A succession of Sirius Group glacigene sediments which crop out along the western margins of the Beardmore valley between Cherry Icefall and Hewson Glacier, below The Cloudmaker, is designated as the stratotype of the Cloudmaker Formation. This new formation overlies a multiply glaciated pavement (Dominion Erosion Surface) cut into the Precambrian Goldie Formation, and is disconformably overlain by the Meyer Desert Formation (Sirius Group). The Cloudmaker Formation comprises bedded and massive diamictons, bedded sands and silts, and laminated clays. Assemblages of foraminifera occur throughout the Cloudmaker Formation and indicate that these basal Sirius Group sediments were deposited in brackish glacial marine environments. The general absence of diatoms suggest these marine waters were ice-covered. Similar marine assemblages are also present in basal Sirius Group sediments at Oliver Bluffs, Dominion Range. Recycled marine diatom assemblages in the Sirius Group at the latter locality indicate that the host sediments have an age of < 3.8 Ma (Pliocene). The Cloudmaker Formation is placed in the Pliocene, although a latest Miocene age for the basal sediments cannot be ruled out.Stratigraphie, sedimentologic, and paleontologic evidence suggests that Beardmore valley was occupied by a fjord and tidewater glacier system that extended at least 165 km through the Transantarctic Mountains from the southwestern Ross Sea. The stratigraphy of The Cloudmaker Formation consists of a succession of members separated by disconformities. It is hypothesised that these strata were deposited by a dynamic valley glacier system that underwent a history of glacier advance and grounding alternating with glacier retreat and flotation over a marine water column. A combination of fjord basin sediment filling and sea-level oscillations may also have influenced the pattern of glacier ice advance and retreat within Beardmore Paleofjord. The marine Cloudmaker Formation is overlain by the terrestrial diamicton dominated Meyer Desert Formation. At Oliver Bluffs, the Meyer Desert Formation diamictons are interbedded with fluvial, and lacustrine sediments; successions that contain in situ vascular plant fossils (principally the Southern Beech Nothofagus), mosses, and beetle remains. A Magellanic-type flora and fauna occupied the coastal margins of the Beardmore Paleofjord. The vertical transition from the basal marine Cloudmaker Formation to terrestrial Meyer Desert Formation provides a sea-level datum that can be used to assess the extent of post-Sirius Group tectonic uplift. Uplift rates at the Cloudmaker section, 90 km inland from the Transantarctic Mountain front or rift shoulder margin in the Queen Alexandra Mountain block are determined to be ~ 429 or ~ 350 m/Myr. This assumes a total uplift of 1331 m for the uppermost marine sediments of the Cloudmaker Formation, and maximum diatom-based ages for the Sirius Group of < 3.1 Ma or < 3.8 Ma. Gross similarities in stratigraphy and interpreted paleoenvironments are apparent between The Cloudmaker succession (Beardmore Paleofjord) and the upper Miocene-Pliocene successions at the mouth of Taylor Paleofjord, 800 km to the north. Contrasting present day elevational settings for these two widely separated marine successions indicates the post-Sirius rate of tectonic uplift for the Transantarctic Mountains has been significantly greater in the Queen Alexandra block.     

Cell Cycle Arrest of Proliferating Neuronal Cells by Serum Deprivation Can Result in Either Apoptosis or Differentiation

Abstract: Apoptotic cell death plays a critical role in the development of the nervous system. The death of mature nondividing neurons that fail to receive appropriate input from the target field has been extensively studied. However, the mechanisms mediating the extensive cell death occurring in areas of the developing brain where proliferating neuroblasts differentiate into mature nondividing neurons have not been analyzed. We show here that the cell cycle arrest of a proliferating cell of neuronal origin by removal of serum results in either apoptotic cell death or differentiation to a mature nondividing neuronal cell. The proportion of cells undergoing death or differentiation is influenced in opposite directions by treatment of the cells with cyclic AMP and retinoic acid. This suggests that following the withdrawal of signals stimulating neuroblast cell division, neuronal cells either can cease to suppress a constitutive suicide pathway and hence die by apoptosis or, alternatively, can differentiate into a mature neuronal cell. Regulation of the balance between apoptosis and neuronal differentiation could therefore play a critical role in controlling the numbers of mature neurons that form.     

The complex genome and adaptive evolution of polyploid Chinese pepper (Zanthoxylum armatum and Zanthoxylum bungeanum)

Zanthoxylum armatum and Zanthoxylum bungeanum, known as ‘Chinese pepper’, are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution and species-special metabolites. Here, we report reference-grade genomes of Z. armatum and Z. bungeanum. Using high coverage sequence data and comprehensive assembly strategies, we derived 66 pseudochromosomes comprising 33 homologous phased groups of two subgenomes, including autotetraploid Z. armatum. The genomic rearrangements and two whole-genome duplications created large (~4.5 Gb) complex genomes with a high ratio of repetitive sequences (>82%) and high chromosome number (2n = 4x = 132). Further analysis of the high-quality genomes shed lights on the genomic basis of involutional reproduction, allomones biosynthesis and adaptive evolution in Chinese pepper, revealing a high consistent relationship between genomic evolution, environmental factors and phenotypic innovation. Our study provides genomic resources and new insights for investigating diversification and phenotypic innovation in Chinese pepper, with broader implications for the protection of plants under severe environmental changes.