全文获取类型
收费全文 | 431篇 |
免费 | 37篇 |
国内免费 | 1篇 |
出版年
2022年 | 4篇 |
2021年 | 19篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 11篇 |
2017年 | 10篇 |
2016年 | 8篇 |
2015年 | 15篇 |
2014年 | 17篇 |
2013年 | 24篇 |
2012年 | 28篇 |
2011年 | 37篇 |
2010年 | 12篇 |
2009年 | 12篇 |
2008年 | 17篇 |
2007年 | 16篇 |
2006年 | 22篇 |
2005年 | 24篇 |
2004年 | 14篇 |
2003年 | 23篇 |
2002年 | 10篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1996年 | 6篇 |
1994年 | 3篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1986年 | 3篇 |
1984年 | 6篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 8篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1975年 | 4篇 |
1967年 | 4篇 |
1963年 | 2篇 |
1961年 | 3篇 |
1960年 | 2篇 |
1956年 | 2篇 |
1950年 | 2篇 |
1948年 | 2篇 |
1941年 | 3篇 |
1932年 | 2篇 |
1931年 | 3篇 |
1930年 | 2篇 |
1929年 | 2篇 |
1927年 | 3篇 |
排序方式: 共有469条查询结果,搜索用时 62 毫秒
1.
Eliot C. Bush Anne E. Clark Chris M. DeBoever Lillian E. Haynes Sidra Hussain Singer Ma Matthew M. McDermott Adam M. Novak John S. Wentworth 《PloS one》2012,7(11)
A significant proportion of enzymes display cooperativity in binding ligand molecules, and such effects have an important impact on metabolic regulation. This is easiest to understand in the case of positive cooperativity. Sharp responses to changes in metabolite concentrations can allow organisms to better respond to environmental changes and maintain metabolic homeostasis. However, despite the fact that negative cooperativity is almost as common as positive, it has been harder to imagine what advantages it provides. Here we use computational models to explore the utility of negative cooperativity in one particular context: that of an inhibitor binding to an enzyme. We identify several factors which may contribute, and show that acting together they can make negative cooperativity advantageous. 相似文献
2.
Lillian Penson 《BMJ (Clinical research ed.)》1950,2(4683):830-832
3.
4.
Neopterin Retards Loss of Serotonin N-Acetyltransferase Activity from Cultured Chick Pineal Glands 总被引:1,自引:1,他引:0
Abstract: d -Neopterin at 10 μ M delayed start of the decline of serotonin N -acetyltransferase (NAT) activity from the peak level in the cycle exhibited by chick pineal glands cultured under standard conditions in the dark. A less marked retardation of decline of NAT activity was found with glands cultured under diurnal illumination or those exposed prematurely to light. There were no significant effects of neopterin on the increases of NAT activity or peak levels of activity developed. The pteridine also retarded loss of NAT activity from the peak level developed in the dark when the time of explanting into culture was later in the (solar) day, but not when it was earlier. Neopterin had no effect on the cycle in cyclic GMP content of cultured chick pineal glands. 相似文献
5.
Lillian Recant Nancy R. Voyles Kim I. Timmers Carla Zalenski Meira Fields S. J. Bhathena 《Peptides》1986,7(6):1061-1069
Free enkephalins (enk) and higher molecular weight enkephalin-containing peptides (enk-c-p) are present in the endocrine pancreas of rats, presumably in B cells. To determine whether these opioid peptides show dynamic alterations as insulin content of pancreas changes, we utilized a copper deficient rat model, in which the exocrine pancreas atrophies and the endocrine pancreas is “intact” and insulin (IRI) content increases. Dietary copper deficiency (−C) was produced in weanling male rats for 4 and 7 weeks. The deficient and copper supplemented (+C) groups were further subdivided to receive all dietary carbohydrate as either 62% fructose (F) or 62% starch (S). −CF rats showed the most severe deficiency. After 7 weeks, total units of pancreatic IRI in −CF were 7.5, +CF 2.1, −CS 7.9 and in +CS 2.8 (p<0.001). Pancreatic content of Met5- and Leu5-enk was measured in extracts which were purified on C-18 Seppaks with and without prior treatment with trypsin and carboxypeptidase B. −C animals showed progressive, significant increases in pancreatic content of Leu-enk-c-p, with a decrease in free Leu- and Met-enk (p<0.02-0.01). The pancreatic findings are compatible with a co-localization of enkephalins and insulin in the endocrine pancreas and are suggestive of co-regulation. 相似文献
6.
7.
Paraskeva Michailova Ninel Petrova Lillian Ramella Gabriella Sella Jordanka Todorova Vincenzo Zelano 《Genetica》1996,98(2):161-178
A population of Chironomus riparius from a Po river station near Moncalieri (a trace-metal polluted station) was studied. In this population was established a great variability of band structure of polytene chromosomes as well as paracentric heterozygous inversions, deletions, deficiencies, partial breaks, diploid chromosome fragments, and changes in functional activity and appearance of heterochromatin. In arms A through F, some bands had an increased size compared to the standard chromosomic map. Some bands appeared in a heterozygous or normal homozygous state or were amplified. In all arms, many condensed stable bands appeared in the decondensed state when compared to the standard map. Asynaptic zones in arms E and G as well as heterozygous Balbiani rings and NORs were established. Very often the 4th chromosome was almost completely heteropycnotic and looded like a pompon chromosome. For the first time in this species, a high frequency of ectopic pairings of different arms was observed. Telomeric regions involved in ectopic pairings had a granular appearance, as did some centromeres. The hypothesis is advanced that such a high frequency of structural rearrangements could be correlated with genomic distribution of specific mobile elements. 相似文献
8.
9.
Borrelia burgdorferi is a spirochete pathogen transmitted among warm-
blooded hosts by ixodid ticks. Frequency-dependent selection for variant
outer-surface proteins might be expected to arise in this species, since
rare variants are more likely to avoid immune surveillance in previously
infected hosts. We sequenced the OspA and OspB genes of nine North American
strains and compared them with nine strains previously described. For each
gene, the mean number of synonymous substitutions per synonymous site and
the mean number of nonsynonymous substitutions per nonsynonymous site show
only a twofold excess of silent mutations. Synonymous rates vary widely
along the OspB protein. Some regions show a significant excess of silent
substitutions, while divergence in other regions is constrained by biased
base composition or selection. The presence, in antigenically important
regions of the protein, of significant variation among strains, as well as
evidence for recombination among strains, should be considered in attempts
to develop vaccines against this disease.
相似文献
10.