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1.
Background
Male circumcision (MC) is an effective strategy to prevent HIV infection in heterosexual men. To our knowledge, there are no studies of the acceptability of this procedure in the Dominican Republic (DR). The main objective of this study was to assess the acceptability of MC to prevent HIV transmission among men ages 18 to 50 years in the Altagracia Province in the Dominican Republic. Because differences in culture and beliefs between Haitians and Dominicans could potentially influence their acceptability of MC, we conducted a comparative analysis based on national origin.Methods
A survey was administered to a convenience sample of 368 men. The questionnaire was divided in 3 sections: 1) Background demographics (including national origin), 2) Male circumcision and 3) Sexual health. Stratified and logistic multivariate regression analyses were performed to identify factors associated with the acceptability of MC.Results
The sample consisted of 238 (65%) Dominicans and 130 (35%) Haitian immigrants. Almost all participants were uncircumcised (95%) and about half (52%) were single. The overall acceptability of MC was 29%. The number of men willing to be circumcised increased to 67% after an information session explaining the benefits of the procedure. 74% of men reported that they would be willing to circumcise their sons after hearing that information. In multivariate analysis, Haitian nationality (OR = 1.86, 95% CI 1.01–3.41), knowing that circumcision improves hygiene (OR = 2.78, 95% CI 1.29–6.0) and not believing that circumcision decreases sexual pleasure (OR = 2.18, 95% CI 1.20–3.94) were associated with a higher acceptability of the procedure. Although age was not significantly associated with the willingness to be circumcised in the multivariate analysis, stratified analysis based on national origin suggested that younger Dominicans (<30 years of age) are more likely to accept the procedure when compared to their older counterparts (OR = 2.17, 95% CI 1.14–4.12).Conclusions
An important number of sexually active men in the DR may be willing to be circumcised if educational resources detailing the benefits of the procedure are made available. These educational activities would constitute a great opportunity to teach about sexual health and reinforce safe sex practices. 相似文献2.
Alkyl hydroperoxide reductase, catalase, MrgA, and superoxide dismutase are not involved in resistance of Bacillus subtilis spores to heat or oxidizing agents. 总被引:2,自引:0,他引:2
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Only a single superoxide dismutase (SodA) was detected in Bacillus subtilis, and growing cells of a sodA mutant exhibited paraquat sensitivity as well as a growth defect and reduced survival at an elevated temperature. However, the sodA mutation had no effect on the heat or hydrogen peroxide resistance of wild-type spores or spores lacking the two major DNA protective alpha/beta-type small, acid-soluble, spore proteins (termed alpha(-)beta(-) spores). Spores also had only a single catalase (KatX), as the two catalases found in growing cells (KatA and KatB) were absent. While a katA mutation greatly decreased the hydrogen peroxide resistance of growing cells, as found previously, katA, katB, and katX mutations had no effect on the heat or hydrogen peroxide resistance of wild-type or alpha(-)beta(-) spores. Inactivation of the mrgA gene, which codes for a DNA-binding protein that can protect growing cells against hydrogen peroxide, also had no effect on spore hydrogen peroxide resistance. Inactivation of genes coding for alkyl hydroperoxide reductase, which has been shown to decrease growing cell resistance to alkyl hydroperoxides, had no effect on spore resistance to such compounds or on spore resistance to heat and hydrogen peroxide. However, Western blot analysis showed that at least one alkyl hydroperoxide reductase subunit was present in spores. Together these results indicate that proteins that play a role in the resistance of growing cells to oxidizing agents play no role in spore resistance. A likely reason for this lack of a protective role for spore enzymes is the inactivity of enzymes within the dormant spore. 相似文献
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4.
Marin MH Rodríguez-Tanty C Higginson-Clarke D Bocalandro YM Peña LP 《Biochemical and biophysical research communications》2005,336(3):983-986
Four chimeric synthetic peptides (Q5, Q6, Q7(multiply sign in circle), and Q8(multiply sign in circle)), incorporating immunodominant epitopes of the core p19 (105-124 a.a.) and envelope gp46 proteins (175-205 a.a.), of HTLV-I were obtained. Also, two gp46 monomeric peptides M4 and M5(multiply sign in circle) (Ser at position 192) were synthesized. The analysis of the influence of the peptide lengths and the proline to serine substitution on the chimeric and monomeric peptides' antigenicity, with regard to the chimeric peptides Q1, Q2, Q3(multiply sign in circle), and Q4(multiply sign in circle), reported previously, for HTLV-I was carried out. The peptides' antigenicity was evaluated in an ultramicroenzyme-linked immunosorbent assay (UMELISA) using sera of HTLV-I/II. The peptides' antigenicity was affected appreciably by the change of the peptide length and amino acid substitutions into the immunodominant sequence of gp46 peptide. 相似文献
5.
Angela P. Cadavid Lilliam Caas John J. Estrada Luis E. Ramirez 《Journal of medical primatology》1991,20(5):259-261
The prevalence of anti-Toxoplasma gondii antibodies was studied in 47 nonhuman primates of the Cebus species in the Santa Fe Zoological Park in Medellín, Colombia. Specific IgG titers (greater than 1/64) were detected in 40.9% of C. albifrons studied (n = 22), 13.3% of C. capucinus (n = 15), and 0% of C. apella (n = 10). Specific IgM was not detected in any of the animals studied. 相似文献
6.
Massimiliano Marvasi Kimberley L. Gallagher Lilliam Casillas Martinez William C. Molina Pagan Ronald E. Rodríguez Santiago Gloria Castilloveitía Vega 《Geomicrobiology journal》2013,30(10):916-924
B4 precipitation medium has been used as the preferred medium for studying mineral precipitation using bacterial strains in vitro since pioneer studies were performed by Boquet and coworkers in 1973. Using this medium, several authors have demonstrated that some environmental isolates were able to precipitate minerals, yet others did not. The main goal of the current study is to understand whether pH and buffer conditions would have a significant effect on mineral precipitation results for environmental isolates grown on B4. For this study, a total of 49 strains isolated from natural environments from Puerto Rico were grown on B4 plates, and their CaCO3 precipitation potential was investigated. Our findings revealed a strong correlation between a lack of CaCO3 precipitation and the acidification of the B4 plates by the colonies. The ability to precipitate CaCO3 could be restored by buffering the B4 medium to a pH of 8.2. Buffering capacity of the medium was proposed to be involved in CaCO3 precipitation: acid-base titrations conducted on the individual ingredients of B4 showed that yeast extract has a poor buffering capacity between pH 6.5–7.5. This pH range corresponds to the pH of B4 plates [6.87 (±0.05)] prior to the inoculation. This might explain why B4 is such a good precipitation medium: a small variation in the H+/OH? balance during microbial growth and precipitation produces rapid changes in the pH of the medium. Finally, an amorphous matrix was distributed within 90% of the examined crystals generated on B4 medium by the environmental strains. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental file. 相似文献
7.
Larvicidal activity against Aedes aegypti of some plants native to the West-Central region of Brazil
Walmir S. Garcez Fernanda R. Garcez Lilliam M.G.E. da Silva Lidilhone Hamerski 《Bioresource technology》2009,100(24):6647-6650
A total of 42 ethanolic extracts from 30 different plant species, native to the Pantanal and Cerrado of the West-Central region of Brazil, have been evaluated for their larvicidal activity against Aedes aegypti larvae, the vector of dengue and dengue hemorrhagic fevers. Among the extracts tested, that obtained from the trunk bark of Ocotea velloziana was the most active. Using a bioassay-directed fractionation of this extract, the active constituent was isolated and characterized as the aporphine alkaloid (+)-dicentrine. Its structure was established on the basis of 1H and 13C NMR spectra, optical rotation and by comparison with an authentic sample. This is the first report on the larvicidal activity against A. aegypti of this alkaloid. Our results suggest that (+)-dicentrine may be considered as a promising natural mosquito larvicidal agent. 相似文献
8.
Marin MH Peña LP Tanty CR Higginson Clarke D Arenas MA Noguerol KR León CS 《Preparative biochemistry & biotechnology》2004,34(3):227-237
The antigenicity of three chimeric synthetic peptides (Qm, Qm-16, and Qm-17) incorporating an immunodominant epitope of the gp41 transmembrane protein (587-617) and the different epitopes of the gp120 envelope protein (495-516), (301-335), (502-516) of human immunodeficiency virus (HIV-1), separated by two glycine residues, was evaluated by UltramicroEnzyme-linked immunosorbent assay (UMELISA) by using panels of anti-HIV-1 positive sera (n = 47). The specificity was evaluated with samples from healthy blood donors (n = 20) and anti-HIV-2 positive samples (n = 10). The results indicate that the chimeric peptide, Qm, was the most reactive one because it detected antibodies to virus efficiently. This may be related to peptide adsorption onto the solid surface, the C-terminal region of HIV-1 gp120 (495-516) combined with gp41 (587-617) in the chimera, and the epitope accessibility to the antibodies. This study showed the usefulness of the chimeric peptides as antigen to detect antibodies to HIV-1 virus. 相似文献
9.
Hernández Marin M Hernández Spengler I Ramos Martínez G Pozo Peña L 《Biochemical and biophysical research communications》2006,339(1):89-92
Six chimeric synthetic peptides (QCha-1, QCha-2, QCha-3, QCha-4, QCha-5, and QCha-6) incorporating antigenic sequences of two immunodominant repeat B-cell epitopes of Trypanosoma cruzi were synthesized by conventional solid-phase peptide synthesis. The antigenic activity of these peptides was evaluated by UltramicroEnzyme-linked immunosorbent assay (UMELISA) by using panels of positive Chagasic sera (n=82), while specificity was evaluated with samples from healthy blood donors (n=44) and patients with other infectious diseases (n=86). The antigenicity of the chimeric peptides in solid-phase immunoassays was compared with that of the monomeric peptides. Data demonstrated that the chimeric peptide QCha-5 was the most reactive because it detected antibodies to parasite efficiently. The results indicate that chimeric peptide as coating antigen is very useful for the immunodiagnosis of Chagas' disease. 相似文献
10.
This study tested expectations of the palatable forage hypothesis for Atta sexdens (L.). Literature records on Atta and Acromyrmex nest density in the Neotropics were analyzed and A. sexdens demographical aspects were studied in six transects located along the Colombian Amazon River. Literature research results did not show that Atta and Acromyrmex nest densities are consistently higher in disturbed habitats. In transects, 56 active and 182 inactive nests were found in 2008. Higher active nest numbers and densities were determined for 1992, 1999, and 2006. Nests were present in old forest, in agricultural plot and grassland secondary forest, and absent from open spaces around houses, grasslands, agricultural plots, and a particular sector of old forest. Nest densities were higher in secondary forest. Between 1992 and 2008, the size of disturbed areas increased, whereas nest numbers and densities diminished. Average nest age was higher in secondary forest, but maximum nest age was comparable in the three habitats. On average, nests were superficially larger in old forest. Nests were mostly established in old forest, at sites without fallen trees. Almost all of those currently present in secondary forest were established in old forest before any disturbance occurred. In disturbed habitats, nest inactivity was slightly higher and possibly affected by human control efforts. This study suggested that the palatable forage hypothesis alone did not explain observed demographic patterns. Soil physiochemical variables or microclimatic conditions also are thought to play important roles in determining A. sexdens demography. 相似文献