ROC曲线分析在评价入侵物种分布模型中的应用

生态位模型（ecological niche models,ENMs）已广泛应用于物种潜在分布区预测,ENMs的应用也为外来入侵物种的风险分析提供了重要的定量化分析工具,但如何评价不同模型之间的预测效果成了当今研究的热点问题。本文介绍了受试者工作特征（ROC）曲线分析在评价不同生态位模型预测效果中的应用原理和分析方法,并以一种植物病原线虫-相似穿孔线虫（Radopholus similis）为例,应用ROC曲线分析法对其5种模型（BIOCLIM,CLIMEX,DOMAIN,GARP,MAXENT）的预测结果进行了比较分析。5种模型的ROC曲线下面积AUC（Area Under Curve）值分别为0.810,0.758,0.921,0.903和0.950,以MAXENT模型的AUC值最大,表明其预测效果最好;方差分析结果表明,除GARP与DOMAIN模型之间AUC值差异不显著外,其余各模型之间差异显著。     

Characterization of Agrobacterium-mediated co-transformation events in rice using green and red fluorescent proteins

Molecular Biology Reports - Biotechnologists seeking to develop marker-free transgenic plants have established co-transformation methods. For co-transformation using mixed Agrobacterium strains,...     

植物抗细菌病害基因工程研究进展和展望

综述了利用基因工程提高植物对细菌病害抗性的各种方法,包括利用非植物抗菌蛋白,抑制细菌的致病或毒性因子,增强植物本身的抗病能力和人工诱导侵染点细胞程序化坏死。这些方法的成功都与抗菌化合物的作用机制及植物和病原细菌之间的相互作用的分子生物学的研究密切相关,还展望了这些方法的应用前景。     

ASK1 physically interacts with COI1 and is required for male fertility inArabidopsis

Jasmonates are a new class of plant hormones that play important roles in plant development and plant defense. TheCOI1 gene was previously shown to be required for jasmonate-regulated plant fertility and defense. We demonstrated for the first time that COI1 interacts with theArabidopsis SKP1-LIKE1 (ASK1) to form a complex that is required for jasmonate action inplanta. Functional analysis by antisense strategy showed thatASK1 is involved in male fertility.     

ASK1 physically interacts with COI1 and is required for male fertility in Arabidopsis

Jasmonates are a new class of plant hormones that play important roles in plant development and plant defense. The COI1 gene was previously shown to be required for jasmonate-regulated plant fertility and defense. We demonstrated for the first time that COI1 interacts with the Arabidopsis SKP1-LIKE1 (ASK1) to form a complex that is required for jasmonate action in planta. Functional analysis by antisense strategy showed that ASK1 is involved in male fertility.     

热激转录因子在植物抗非生物胁迫中的功能研究进展

植物在遭受环境胁迫时会产生一系列应激反应,而热激转录因子可通过介导热激蛋白或其他热诱导基因的转录和表达,来参与调控植物抵抗逆境胁迫过程和其他生命活动。主要介绍了植物热激转录因子的基本蛋白结构域,阐述了3类热激转录因子在抗极端温度（高温、低温）胁迫、干旱胁迫、高盐胁迫、活性氧胁迫中的功能与作用机制,并探讨和展望了植物热激转录因子在植物育种和提高植物抗逆性的研究中的发展与应用前景,以期为深入研究热激转录因子在调控植物抵抗逆境胁迫中的生物学功能与机制提供理论参考。     

水稻广谱抗稻瘟病基因研究进展

稻瘟病是水稻生产中的最严重病害之一,由于稻瘟菌小种的高度变异性,垂直抗性基因难以持续控制稻瘟病的危害,因此,克隆和利用广谱持久抗瘟基因被认为是解决稻瘟病问题最经济有效的策略。本文从广谱抗源的筛选与利用,广谱抗瘟基因的定位、克隆与应用等方面对水稻广谱抗稻瘟病基因研究取得的进展进行了概述,并介绍了广谱抗性分子机理的最新研究进展。基于国内外稻瘟病抗性基因研究的现状及趋势,以及我国丰富的抗瘟水稻种质资源,克隆越来越多的广谱抗瘟基因具有重要的理论与应用价值。     

COS1: an Arabidopsis coronatine insensitive1 suppressor essential for regulation of jasmonate-mediated plant defense and senescence

The Arabidopsis thaliana CORONATINE INSENSITIVE1 (COI1) gene encodes an F-box protein to assemble SCF(COI1) complexes essential for response to jasmonates (JAs), which are a family of plant signaling molecules required for many essential functions, including plant defense and reproduction. To better understand the molecular basis of JA action, we screened for suppressors of coi1 and isolated a coi1 suppressor1 (cos1) mutant. The cos1 mutation restores the coi1-related phenotypes, including defects in JA sensitivity, senescence, and plant defense responses. The COS1 gene was cloned through a map-based approach and found to encode lumazine synthase, a key component in the riboflavin pathway that is essential for diverse yet critical cellular processes. We demonstrated a novel function for the riboflavin pathway that acts downstream of COI1 in the JA signaling pathway and is required for suppression of the COI1-mediated root growth, senescence, and plant defense.