排序方式: 共有162条查询结果,搜索用时 15 毫秒
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Yanbing Wang Yingnan Hou Hongya Gu Dingming Kang Zhang-Liang Chen Jingjing Liu Li-Jia Qu 《植物学报(英文版)》2013,55(1):64-74
Anaphase-promoting complex/cyclosome (APC/C), a multisubunit E3 ligase, plays a critical role in cell cycle control, but the functional characterization of each subunit has not yet been completed. To investigate the function of APC1 in Arabidopsis, we analyzed four mutant alleles of APC1, and found that mutation in APC1 resulted in significantly reduced plant fertility, accumulation of cyclin B, and disrupted auxin distribution in embryos. The three mutant alleles apc1-1, apc1-2 and apc1-3 shared variable defects in female gametogenesis including degradation, abnormal nuclear number, and disrupted polarity of nuclei in the embryo sac as well as in embryogenesis, in which embryos were arrested at multiple stages. All of these defects are similar to those previously identified in apc4. The mutant apc1-4, in which the T-DNA was inserted after the transmembrane domain at the C-terminus, showed much more severe phenotypes; that is, most of the ovules were arrested at the one-nucleate female gametophyte stage (stage FG1). In the apc1 apc4 double mutants, the fertility was further reduced by one-third in apc1-1/+ apc4-1/+, and in some cases no ovules even survived in siliques of apc1-4/+ apc4-1/+. Our data thus suggest that APC1, an essential component of APC/C, plays a synergistic role with APC4 both in female gametogenesis and in embryogenesis. 相似文献
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Hormonal Regulation of Leaf Morphogenesis in Arabidopsis 总被引:1,自引:0,他引:1
Lin-Chuan Li Ding-Ming Kang Zhang-Liang Chen Li-Jia Qu 《植物学报(英文版)》2007,49(1):75-80
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目的和方法:比较自发性高血压大鼠(SHR)和对照(WKY)大鼠心脏和主动脉丝裂素活化蛋白激酶磷酸酶-1(MKP-1)及细胞外信号调节激酶(ERK-1)的表达,并观察用磷酸钙共沉淀方法转染MKP-1基因对血管紧张素Ⅱ(Ang Ⅱ)刺激平滑肌细胞(VSMC)^3H-胸腺叫啶(^3H-TdR)掺入的影响,以探讨MKP-1在细胞增殖中的调节作用。结果:①与WKY大鼠相比,SHR心脏和主动脉MKP-1呈低表达,分别降低53%和45%(P均<0.01);而SHR心脏和主动脉ERK-1呈明显高表达(P均<0.01),SHR心脏和主动脉ERK-1与MKP-1蛋白比值明显高于WKY。②AngⅡ 10^-7mol/L刺激VSMC增殖较对照组增加257%(P<0.01),转染野生型MKP-1基因细胞可使AngⅡ刺激的^3H-TdR掺入较未转染的细胞降低63%(P<0.05),转染突变型MKP-1基因和转染空载体的VSMC对AngⅡ的刺激与单纯AngⅡ组相比无明显抑制作用(P>0.05)。结论:SHR心血管组织中促增殖肥大的ERK-1表达较其失活的MKP-1占优势,并且MKP-1可显著抑制AngⅡ的VSMC增殖。 相似文献
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Li-Jia Xu Feng Huang Si-Bao Chen Lian-Niang Li Shi-Lin Chen Pei-Gen Xiao 《植物学报(英文版)》2006,48(12):1493-1497
In the course of our study of bioactive natural products from Schisandra plants, we isolated a neolignan from an EtOAc extract of the stems of Schisandra propinqua (Wall.) Baill. The structure of the new com- pound was determined to be 4, 4-di (4-hydroxy-3-methoxyphenly)-2, 3-dimethylbutanol (compound 1) on the basis of 1H- and 13C-NMR spectra and 2D NMR methods. Eight known compounds, compounds 2-9, were also isolated and identified, of which compounds 3, 4, 6 and 9 were isolated for the first time from this plant. In addition, compounds 1-4 were evaluated for cytotoxicity by an 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H- tetrazolium bromide (MTT) assay. Compound 1 showed significant potential cytotoxic ability in the bioassay. 相似文献
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大鼠脑内caveolin-1蛋白的表达及其在分辨学习中的作用 总被引:5,自引:0,他引:5
Caveolin-1(Cav—1)蛋白作为细胞质膜结构小窝(caveolae)的标志蛋白,在胆固醇运输、膜组装、信号转导和细胞转化过程中扮演重要的角色。为了探讨Cav-1蛋白在中枢神经系统可塑性及学习记忆中的作用,本文以Sprague—Dawley大鼠为实验对象,利用蛋白质免疫印迹杂交方法观察了Car-1蛋白在不同年龄大鼠脑内表达的特征,并研究了Y-迷宫训练前后Cav-1蛋白表达的变化。结果表明:(1)大鼠不同脑区Cav-1蛋白表达的年龄特征不同。海马内的表达属青年鼠最高,其次是老年鼠和幼年鼠;皮层内的表达属幼年鼠最高,其次是老年鼠,青年鼠最低;小脑内的表达无明显年龄差异。(2)Y-迷宫训练引起青年鼠海马和前额叶皮层内Cav-1蛋白的表达显著增加。结果提示,Cav-1蛋白与动物脑发育和学习记忆有密切关系,可能参与中枢可塑性的调节。 相似文献
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鱼类生殖细胞移植的研究进展 总被引:1,自引:0,他引:1
生殖细胞移植在生物学、畜牧学及兴起的鱼类生物工程上都有很多用途。目前,采用基于基因组的育种方法已选育出多种带有所需遗传性状的鱼类新品系;但由于缺乏鱼卵与胚胎的低温保存技术,尚未找到长期保存其种质资源的方法。就鱼类生殖细胞移植的最新研究进展进行综述,同时针对鱼类生殖细胞移植及其低温保存技术研究中存在的问题进行讨论,并提出了鱼类生殖细胞移植的应用前景,以期能积极推动鱼类生物工程的研究,加速鱼类生殖细胞移植在产业中的应用。 相似文献