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1.
Maroun RG Krebs D El Antri S Deroussent A Lescot E Troalen F Porumb H Goldberg ME Fermandjian S 《The Journal of biological chemistry》1999,274(48):34174-34185
EAA26 (VESMNEELKKIIAQVRAQAEHLKTAY) is a better inhibitor of human immunodeficiency virus, type 1, integrase than its parent Lys-159, reproducing the enzyme segment 147-175 with a nonpolar-polar/charged residue periodicity defined by four helical heptads (abcdefg) prone to collapse into a coiled-coil. Circular dichroism, nuclear magnetic resonance, sedimentation equilibrium, and chemical cross-linking were used to analyze EAA26 in various trifluoroethanol/H(2)O mixtures. In pure water the helix content is weak but increases regularly up to 50-60% trifluoroethanol. In contrast the multimerization follows a bell-shaped curve with monomers in pure water, tetramers at 10% trifluoroethanol, and dimers at 40% trifluoroethanol. All suggest that interhelical interactions between apolar side chains are required for the coiled-coil formation of EAA26 and subsist at medium trifluoroethanol concentration. The N(H) temperature coefficients measured by nuclear magnetic resonance show that at low trifluoroethanol concentration the amide groups buried in the hydrophobic interior of four alpha-helix bundles are weakly accessible to trifluoroethanol and are only weakly subject to its hydrogen bond strengthening effect. The increased accessibility of trifluoroethanol to buried amide groups at higher trifluoroethanol concentration entails the reduction of the hydrophobic interactions and the conversion of helix tetramers into helix dimers, the latter displaying a smaller hydrophobic interface. The better inhibitory activity of EAA26 compared with Lys-159 could arise from its better propensity to form a helix bundle structure with the biologically important helical part of the 147-175 segment in integrase. 相似文献
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Lescot M Rombauts S Zhang J Aubourg S Mathé C Jansson S Rouzé P Boerjan W 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,109(1):10-22
Poplar has become a model system for functional genomics in woody plants. Here, we report the sequencing and annotation of the first large contiguous stretch of genomic sequence (95 kb) of poplar, corresponding to a bacterial artificial chromosome clone mapped 0.6 centiMorgan from the Melampsora larici-populina resistance locus. The annotation revealed 15 putative genetic objects, of which five were classified as hypothetical genes that were similar only with expressed sequence tags from poplar. Ten putative objects showed similarity with known genes, of which one was similar to a kinase. Three other objects corresponded to the toll/interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat class of plant disease resistance genes, of which two were predicted to encode an amino terminal nuclear localization signal. Four objects were homologous to the Ty1/copia family of class I transposable elements, one of which was designated Retropop and interrupted one of the disease resistance genes. Two other objects constituted a novel Spm-like class II transposable element, which we designated Magali.M.L. and S.R. contributed equally to this article 相似文献
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Peter von Dassow Uwe John Hiroyuki Ogata Ian Probert El Mahdi Bendif Jessica U Kegel Stéphane Audic Patrick Wincker Corinne Da Silva Jean-Michel Claverie Scott Doney David M Glover Daniella Mella Flores Yeritza Herrera Magali Lescot Marie-José Garet-Delmas Colomban de Vargas 《The ISME journal》2015,9(6):1365-1377
Emiliania huxleyi is the most abundant calcifying plankton in modern oceans with substantial intraspecific genome variability and a biphasic life cycle involving sexual alternation between calcified 2N and flagellated 1N cells. We show that high genome content variability in Emiliania relates to erosion of 1N-specific genes and loss of the ability to form flagellated cells. Analysis of 185 E. huxleyi strains isolated from world oceans suggests that loss of flagella occurred independently in lineages inhabiting oligotrophic open oceans over short evolutionary timescales. This environmentally linked physiogenomic change suggests life cycling is not advantageous in very large/diluted populations experiencing low biotic pressure and low ecological variability. Gene loss did not appear to reflect pressure for genome streamlining in oligotrophic oceans as previously observed in picoplankton. Life-cycle modifications might be common in plankton and cause major functional variability to be hidden from traditional taxonomic or molecular markers. 相似文献
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Thijs G Moreau Y De Smet F Mathys J Lescot M Rombauts S Rouze P De Moor B Marchal K 《Bioinformatics (Oxford, England)》2002,18(2):331-332
INCLUSive allows automatic multistep analysis of microarray data (clustering and motif finding). The clustering algorithm (adaptive quality-based clustering) groups together genes with highly similar expression profiles. The upstream sequences of the genes belonging to a cluster are automatically retrieved from GenBank and can be fed directly into Motif Sampler, a Gibbs sampling algorithm that retrieves statistically over-represented motifs in sets of sequences, in this case upstream regions of co-expressed genes. 相似文献
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Synthesis and biological evaluation of thienopyrrolizines, a new family of CDK/GSK-3 inhibitors 总被引:1,自引:0,他引:1
Rochais C Lescot E Lisowski V Lepailleur A Santos JS Bureau R Dallemagne P Meijer L Rault S 《Journal of enzyme inhibition and medicinal chemistry》2004,19(6):585-593
Fifteen new thieno[2,3-b ]- and thieno[3,4-b]pyrrolizines were synthesized and tested against two protein kinases, CDK1/cyclin B and GSK-3. Among these compounds, 3-(3-hydroxy-4-methoxyphenyl)-8H-thieno[2,3-b]pyrrolizin-8-one 4g was identified as a moderate inhibitor of these kinases. Its molecular modeling study brought to the fore the pivotal role of the 2-methoxyphenol grouping and the interest in replacing it by bioisosteric moieties in future pharmacomodulations. 相似文献
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Recovery and regeneration of embryogenic cultures from female flowers of False Horn Plantain 总被引:7,自引:0,他引:7
Grapin A. Ortíz J-L. Lescot T. Ferrière N. Côte F.X. 《Plant Cell, Tissue and Organ Culture》2000,61(3):237-244
Somatic embryogenesis from immature male flowers in Musa is only suitable for genotypes with a male bud. Six friable embryogenic cultures were obtained from 28 cultured buds of female
flowers of the AAB False Horn Plantains, ‘Curraré’ and ‘Curraré Enano’. Embryogenic suspensions were established from these
embryogenic cultures. Somatic embryogenesis was demonstrated histologicaly. Regeneration of plants was obtained either from
somatic embryos directly isolated from embryogenic cultures or from suspensions after plating on a semi-solid medium. This
study demonstrates that somatic embryogenesis from immature flowers is suitable for genotypes of Musa with or without male buds.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Christophe Rochais Elodie Lescot Vincent Lisowski Alban Lepailleur Jana sopkova-de oliveira Santos Ronan Bureau 《Journal of enzyme inhibition and medicinal chemistry》2013,28(6):585-593
Fifteen new thieno[2,3-b]- and thieno[3,4-b]pyrrolizines were synthesized and tested against two protein kinases, CDK1/cyclin B and GSK-3. Among these compounds, 3-(3-hydroxy-4-methoxyphenyl)-8H-thieno[2,3-b]pyrrolizin-8-one 4g was identified as a moderate inhibitor of these kinases. Its molecular modeling study brought to the fore the pivotal role of the 2-methoxyphenol grouping and the interest in replacing it by bioisosteric moieties in future pharmacomodulations. 相似文献