全文获取类型
收费全文 | 3600篇 |
免费 | 313篇 |
专业分类
3913篇 |
出版年
2024年 | 5篇 |
2023年 | 31篇 |
2022年 | 79篇 |
2021年 | 119篇 |
2020年 | 93篇 |
2019年 | 114篇 |
2018年 | 124篇 |
2017年 | 112篇 |
2016年 | 154篇 |
2015年 | 217篇 |
2014年 | 222篇 |
2013年 | 271篇 |
2012年 | 310篇 |
2011年 | 289篇 |
2010年 | 189篇 |
2009年 | 119篇 |
2008年 | 220篇 |
2007年 | 164篇 |
2006年 | 180篇 |
2005年 | 135篇 |
2004年 | 125篇 |
2003年 | 105篇 |
2002年 | 90篇 |
2001年 | 33篇 |
2000年 | 33篇 |
1999年 | 40篇 |
1998年 | 22篇 |
1997年 | 20篇 |
1996年 | 19篇 |
1995年 | 17篇 |
1994年 | 18篇 |
1993年 | 15篇 |
1992年 | 21篇 |
1991年 | 26篇 |
1990年 | 18篇 |
1989年 | 16篇 |
1988年 | 14篇 |
1987年 | 19篇 |
1986年 | 10篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1983年 | 12篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1979年 | 7篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1975年 | 7篇 |
1972年 | 8篇 |
1970年 | 6篇 |
排序方式: 共有3913条查询结果,搜索用时 15 毫秒
1.
2.
Biogeographical analyses are applied to skipper (Hesperioidea) presence/absence data from the Western Mediterranean mainland
and the three largest islands (Sardinia, Corsica and Sicily) in order to identify potential conservation issues. The analyses
performed on species, both collectively and individually, indicate that regional species richness and occurrence in the Mediterranean
zone are largely predicted by latitude and area but that islands have impoverished faunas. Several species, predicted to be
present on these islands from logistic regression of their continental distributions, are actually absent. The number of species
predicted to be present from logistic regression analyses for each island, closely matched the number of species predicted
to occur in regional-focused multiple regression analysis. This suggests that missing species have been identified. When compared
with species that occur in Sicily and Corsica, the missing species are shown to differ for ecological traits, mainly those
linked to altitudinal tolerance. No ecological distinctions were disclosed for Sardinian skippers suggesting a mainly stochastic
colonisation. These results, and those from an analogous study carried out on Papilionoidea, point to Hesperioidea having
(i) overall more impoverished faunas on islands and (ii) being subject to stochastic or historical colonisation events more
than Papilionoidea. Species not predicted to occur on islands based on their mainland distributions and ecological traits,
are foci for conservation attention. However, as many species becoming extinct on the islands may be irreplaceable, all species,
in particular the Sardinian ones, deserve to be conserved. 相似文献
3.
Serum proteins [molecular weight (MW) > 10,000] are essential for increased insulin-stimulated glucose transport after in vitro muscle contractions. We investigated the role of the kallikrein-kininogen system, including bradykinin, which is derived from kallikrein (MW > 10,000)-catalyzed degradation of serum protein kininogen (MW > 10,000), on this contraction effect. In vitro electrical stimulation of rat epitrochlearis muscles was performed in 1) rat serum +/- kallikrein inhibitors; 2) human plasma (normal or kallikrein-deficient); 3) rat serum +/- bradykinin receptor-2 inhibitors; or 4) serum-free buffer +/- bradykinin. 3-O-methylglucose transport (3-MGT) was measured 3.5 h later. Serum +/- kallikrein inhibitors tended (P = 0.08) to diminish postcontraction insulin-stimulated 3-MGT. Contractions in normal plasma enhanced insulin-stimulated 3-MGT vs. controls, but contractions in kallikrein-deficient plasma did not. Supplementing rat serum with bradykinin receptor antagonist HOE-140 during contraction did not alter insulin-stimulated 3-MGT. Muscles stimulated to contract in serum-free buffer plus bradykinin did not have enhanced insulin-stimulated 3-MGT. Bradykinin was insufficient for postcontraction-enhanced insulin sensitivity. However, results with kallikrein inhibitors and kallikrein-deficient plasma suggest kallikrein plays a role in this improved insulin action. 相似文献
4.
5.
6.
7.
The systemic injection of kainic acid (KA) has been shown to destroy neurons in the hippocampus and to induce limbic-type seizure activity. However, little is known on the neurochemical events that are associated with this convulsant effect. In the present work we studied the spontaneous and the K+-stimulated release of labeled -aminobutyric acid (GABA), glutamate, serotonin and dopamine, in hippocampal slices of KA-treated rats, at the moment of clinical seizures (2 h) and 72 h later. At the onset of convulsions we found a 40–45% decrease in the K+-stimulated release of GABA. The release of the other neurotransmitters was not significantly affected by KA treatment. After 72 h GABA release was still reduced by 30–40%. It is concluded that the epileptogenic effect of KA in the hippocampus is probably related to a diminished inhibitory GABAergic neurotransmission. 相似文献
8.
The amino-terminal half of rotavirus SA114fM VP4 protein contains a hemagglutination domain and primes for neutralizing antibodies to the virus. 总被引:11,自引:9,他引:2 下载免费PDF全文
We have previously reported the synthesis in Escherichia coli of polypeptide MS2-VP8', which contains the amino-terminal half of the SA114fM VP4 protein fused to MS2 bacteriophage polymerase sequences (C. F. Arias, M. Lizano, and S. López, J. Gen. Virol. 68:633-642, 1987). In this work we have synthesized the carboxy-terminal half of the VP4 protein also fused to the MS2 polymerase. This protein, designated MS2-VP5', was recognized by sera to the complete virion and was able to induce antibodies to the virus when administered to mice; however, these antibodies had no neutralizing activity. The two chimeric polypeptides were tested for their ability to agglutinate erythrocytes and to prime the immune system of mice. Bacterial lysates enriched for the MS2-VP8' hybrid polypeptide, but not those enriched for the MS2-VP5' protein or those containing proteins from the host E. coli strain, had hemagglutinating activity. This hemagglutination was inhibited by sera to SA114fM rotavirus. In addition, a single dose of the MS2-VP8' polypeptide was able to prime the immune system of mice for an augmented neutralizing antibody response when the animals were subsequently immunized with purified SA114fM virus. 相似文献
9.
Distinctions between the multiple cationic forms of rat liver glutathione S-transferase 总被引:3,自引:0,他引:3
Three cationic glutathione S-transferase forms isolated from rat liver were characterized as dimers that originated from different combinations of two subunit types, Ya and Yc. The cationic forms were purified using lysyl glutathione affinity matrices and were chromatographically resolved from anionic glutathione S-transferases that contain Yb subunits. The three classes of cationic transferase exhibited similar specific activities with 1-chloro-2,4-dinitrobenzene as a substrate, all forms cross-reacted with antibodies to glutathione S-transferase B, and all had comparable secondary structures and tryptophan fluorescence properties. In spite of those similarities, the Yc-containing forms were clearly distinguishable from Ya forms on the basis of characteristic differences in circular dichroic patterns associated with their aromatic side chains. All cationic transferases bound bilirubin with stoichiometric ratios of 1 mol/dimeric protein molecule, but discrete differences in mode of binding were ascribed to forms containing Ya subunits as compared to Yc dimers. Binding to Yc forms was of lower affinity and may be associated with the catalytic region of the protein since glutathione effectively displaced bilirubin from the Yc component. 相似文献
10.
The sinusoidal domain of the plasma membrane of rat hepatocytes contains an amiloride-sensitive Na+/H+ antiport 总被引:4,自引:0,他引:4
ATP-dependent trapping of [14C]methylamine was demonstrated in vesicles selectively derived from the sinusoidal plasma membrane of rat hepatocytes; activity was lacking in vesicles prepared from the canalicular domain of the plasma membrane of rat hepatocytes. The proton movement was inhibited by carbonyl cyanide p-trifluoromethoxyphenylhydrazone, strophanthidin, vanadate, amiloride, and absence of sodium. 22Na efflux from sinusoidal membrane vesicles increased inversely to extravesicular pH. The results indicate that the sinusoidal plasma membrane of rat hepatocytes contains a Na+/H+ antiport. 相似文献