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Violetta R. Beklemisheva Polina L. Perelman Natalya A. Lemskaya Anastasia I. Kulemzina Anastasia A. Proskuryakova Vladimir N. Burkanov Alexander S. Graphodatsky 《PloS one》2016,11(1)
Karyotype evolution in Carnivora is thoroughly studied by classical and molecular cytogenetics and supplemented by reconstructions of Ancestral Carnivora Karyotype (ACK). However chromosome painting information from two pinniped families (Odobenidae and Otariidae) is noticeably missing. We report on the construction of the comparative chromosome map for species from each of the three pinniped families: the walrus (Odobenus rosmarus, Odobenidae–monotypic family), near threatened Steller sea lion (Eumetopias jubatus, Otariidae) and the endemic Baikal seal (Pusa sibirica, Phocidae) using combination of human, domestic dog and stone marten whole-chromosome painting probes. The earliest karyological studies of Pinnipedia showed that pinnipeds were characterized by a pronounced karyological conservatism that is confirmed here with species from Phocidae, Otariidae and Odobenidae sharing same low number of conserved human autosomal segments (32). Chromosome painting in Pinnipedia and comparison with non-pinniped carnivore karyotypes provide strong support for refined structure of ACK with 2n = 38. Constructed comparative chromosome maps show that pinniped karyotype evolution was characterized by few tandem fusions, seemingly absent inversions and slow rate of genome rearrangements (less then one rearrangement per 10 million years). Integrative comparative analyses with published chromosome painting of Phoca vitulina revealed common cytogenetic signature for Phoca/Pusa branch and supports Phocidae and Otaroidea (Otariidae/Odobenidae) as sister groups. We revealed rearrangements specific for walrus karyotype and found the chromosomal signature linking together families Otariidae and Odobenidae. The Steller sea lion karyotype is the most conserved among three studied species and differs from the ACK by single fusion. The study underlined the strikingly slow karyotype evolution of the Pinnipedia in general and the Otariidae in particular. 相似文献
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Kartavtseva I. V. Vasilieva T. V. Sheremetyeva I. N. Lemskaya N. A. Moroldoev I. V. Golenishchev F. N. 《Russian Journal of Genetics》2019,55(8):978-992
Russian Journal of Genetics - In the present study, on the basis of cytogenetic and molecular genetic (mtDNA control region) analysis, genetic variability of the Muya valley vole, Alexandromys... 相似文献
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Romanenko S. A. Lemskaya N. A. Beklemisheva V. P. Perelman P. L. Serdukova N. A. Graphodatsky A. S. 《Russian Journal of Genetics》2010,46(9):1138-1142
Here, we present analysis of data on comparative chromosome painting produced using various chromosome-specific libraries
for members of different Glires groups. Based on the results of comparative cytogenetic and molecular studies, the modern
rodents can be conventionally classified into two groups with sharply differing directions and tempos of karyotypic evolution.
One group (suborders Sciuromorpha, Castorimorpha, and Anomaluromorpha) preserved conserved genomes, which are probably close
in structure to the genome of the ancestor of all mammals. The genomes of the other group (suborder Myomorpha) underwent “catastrophic
evolution,” which resulted in numerous breaks and fusions of the ancient chromosomes. The current data do not allow unambiguously
assigning the order Hystricomorpha to any of these groups. 相似文献
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Fragile X syndrome is one of the most common reasons for human hereditary mental retardation. It is associated with the expansion of CGG repeats in the 5'-untranslated region of the FMR1 gene, which results in the suppression of its expression and the development of the disease. At present, methods based on PCR and Southern blot analysis are used for diagnostics of the fragile X syndrome. The presence of a fragile site FRAXA on the X chromosome is typical for patients with this pathology. We developed a method of visualizing this site in cell cultures obtained from patients using the fluorescent in situ hybridization (FISH) and the combination of two probes. The method allows one to detect five types of signals on the X chromosome, three of which are normal, while two are associated with the emergence of fragile site FRAXA. An analysis of the distribution of all signal types in cell lines from healthy individuals and patients with fragile X syndrome demonstrated that the method allows one to determine differences between lines with a high statistical significance and that it is applicable to detecting cells that are carriers of the syndrome. 相似文献
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Biltueva L. S. Perelman P. L. Proskuryakova A. A. Lemskaya N. A. Serdyukova N. A. Grafodatsky A. S. 《Cell and Tissue Biology》2020,14(6):407-412
Cell and Tissue Biology - The chromosomes of the Indian muntjac (Muntiacus muntjak, 2n = 6 in females and 2n = 7 in males) are described using the methods of G-, C-, CDAG-, and AgNOR-staining, and... 相似文献
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